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Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli
Learning by building is one of the core ideas of synthetic biology research. Consequently, building synthetic chromosomes is the way to fully understand chromosome characteristics. The last years have seen exciting synthetic chromosome studies. We had previously introduced the synthetic secondary ch...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5179572/ https://www.ncbi.nlm.nih.gov/pubmed/28066763 http://dx.doi.org/10.3389/fbioe.2016.00096 |
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author | Messerschmidt, Sonja J. Schindler, Daniel Zumkeller, Celine M. Kemter, Franziska S. Schallopp, Nadine Waldminghaus, Torsten |
author_facet | Messerschmidt, Sonja J. Schindler, Daniel Zumkeller, Celine M. Kemter, Franziska S. Schallopp, Nadine Waldminghaus, Torsten |
author_sort | Messerschmidt, Sonja J. |
collection | PubMed |
description | Learning by building is one of the core ideas of synthetic biology research. Consequently, building synthetic chromosomes is the way to fully understand chromosome characteristics. The last years have seen exciting synthetic chromosome studies. We had previously introduced the synthetic secondary chromosome synVicII in Escherichia coli. It is based on the replication mechanism of the secondary chromosome in Vibrio cholerae. Here, we present a detailed analysis of its genetic characteristics and a selection approach to optimize replicon stability. We probe the origin diversity of secondary chromosomes from Vibrionaceae by construction of several new respective replicons. Finally, we present a synVicII version 2.0 with several innovations including its full compatibility with the popular modular cloning (MoClo) assembly system. |
format | Online Article Text |
id | pubmed-5179572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51795722017-01-06 Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli Messerschmidt, Sonja J. Schindler, Daniel Zumkeller, Celine M. Kemter, Franziska S. Schallopp, Nadine Waldminghaus, Torsten Front Bioeng Biotechnol Bioengineering and Biotechnology Learning by building is one of the core ideas of synthetic biology research. Consequently, building synthetic chromosomes is the way to fully understand chromosome characteristics. The last years have seen exciting synthetic chromosome studies. We had previously introduced the synthetic secondary chromosome synVicII in Escherichia coli. It is based on the replication mechanism of the secondary chromosome in Vibrio cholerae. Here, we present a detailed analysis of its genetic characteristics and a selection approach to optimize replicon stability. We probe the origin diversity of secondary chromosomes from Vibrionaceae by construction of several new respective replicons. Finally, we present a synVicII version 2.0 with several innovations including its full compatibility with the popular modular cloning (MoClo) assembly system. Frontiers Media S.A. 2016-12-23 /pmc/articles/PMC5179572/ /pubmed/28066763 http://dx.doi.org/10.3389/fbioe.2016.00096 Text en Copyright © 2016 Messerschmidt, Schindler, Zumkeller, Kemter, Schallopp and Waldminghaus. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Messerschmidt, Sonja J. Schindler, Daniel Zumkeller, Celine M. Kemter, Franziska S. Schallopp, Nadine Waldminghaus, Torsten Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli |
title | Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli |
title_full | Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli |
title_fullStr | Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli |
title_full_unstemmed | Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli |
title_short | Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli |
title_sort | optimization and characterization of the synthetic secondary chromosome synvicii in escherichia coli |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5179572/ https://www.ncbi.nlm.nih.gov/pubmed/28066763 http://dx.doi.org/10.3389/fbioe.2016.00096 |
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