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The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma
TRPM2, one member of the transient receptor potential (TRP) protein super-family, is a Ca(2+)-permeable channel that is activated by oxidative stress and confers susceptibility to cell death. In the human tongue specimens of carcinoma and the tongue carcinoma SCC cell lines, we observed the enhanced...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5180100/ https://www.ncbi.nlm.nih.gov/pubmed/28008929 http://dx.doi.org/10.1038/srep38471 |
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author | Zhao, Ling-Yan Xu, Wan-Lin Xu, Zeng-Qi Qi, Cui Li, Yang Cheng, Jie Liu, Lai-Kui Wu, Yu-Nong Gao, Jun Ye, Jin-Hai |
author_facet | Zhao, Ling-Yan Xu, Wan-Lin Xu, Zeng-Qi Qi, Cui Li, Yang Cheng, Jie Liu, Lai-Kui Wu, Yu-Nong Gao, Jun Ye, Jin-Hai |
author_sort | Zhao, Ling-Yan |
collection | PubMed |
description | TRPM2, one member of the transient receptor potential (TRP) protein super-family, is a Ca(2+)-permeable channel that is activated by oxidative stress and confers susceptibility to cell death. In the human tongue specimens of carcinoma and the tongue carcinoma SCC cell lines, we observed the enhanced expression of TRPM2. By means of the whole-cell electrophysiological recording, the ADPR-induced currents mediated by TRPM2 were recorded in cultured SCC9 cells. Moreover, after H(2)O(2) treatment for 24 hours, the apoptotic number of SCC9 cells was significantly increased. However, the selectively knocked-down TRPM2 with the small interfering RNA technique inhibited the survival and migration of the SCC9 cancer cells, which was independent of the p53-p21 pathway, since the expression of p21 was enhanced after TRPM2 knockdown. Furthermore, the sub-cellular localization of TRPM2 was remarkably different between cancerous and non-cancerous cells. A significant amount of the TRPM2 proteins were located in the nuclei in cancer cells. All these data suggest that TRPM2 is essential for the survival and migration of SCC cancer cells and may be a potential target for the selective treatment of tongue cancer. |
format | Online Article Text |
id | pubmed-5180100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51801002016-12-29 The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma Zhao, Ling-Yan Xu, Wan-Lin Xu, Zeng-Qi Qi, Cui Li, Yang Cheng, Jie Liu, Lai-Kui Wu, Yu-Nong Gao, Jun Ye, Jin-Hai Sci Rep Article TRPM2, one member of the transient receptor potential (TRP) protein super-family, is a Ca(2+)-permeable channel that is activated by oxidative stress and confers susceptibility to cell death. In the human tongue specimens of carcinoma and the tongue carcinoma SCC cell lines, we observed the enhanced expression of TRPM2. By means of the whole-cell electrophysiological recording, the ADPR-induced currents mediated by TRPM2 were recorded in cultured SCC9 cells. Moreover, after H(2)O(2) treatment for 24 hours, the apoptotic number of SCC9 cells was significantly increased. However, the selectively knocked-down TRPM2 with the small interfering RNA technique inhibited the survival and migration of the SCC9 cancer cells, which was independent of the p53-p21 pathway, since the expression of p21 was enhanced after TRPM2 knockdown. Furthermore, the sub-cellular localization of TRPM2 was remarkably different between cancerous and non-cancerous cells. A significant amount of the TRPM2 proteins were located in the nuclei in cancer cells. All these data suggest that TRPM2 is essential for the survival and migration of SCC cancer cells and may be a potential target for the selective treatment of tongue cancer. Nature Publishing Group 2016-12-23 /pmc/articles/PMC5180100/ /pubmed/28008929 http://dx.doi.org/10.1038/srep38471 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhao, Ling-Yan Xu, Wan-Lin Xu, Zeng-Qi Qi, Cui Li, Yang Cheng, Jie Liu, Lai-Kui Wu, Yu-Nong Gao, Jun Ye, Jin-Hai The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma |
title | The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma |
title_full | The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma |
title_fullStr | The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma |
title_full_unstemmed | The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma |
title_short | The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma |
title_sort | overexpressed functional transient receptor potential channel trpm2 in oral squamous cell carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5180100/ https://www.ncbi.nlm.nih.gov/pubmed/28008929 http://dx.doi.org/10.1038/srep38471 |
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