Cargando…

Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil

Nitrous oxide (N(2)O) is a potent greenhouse gas that is produced during microbial nitrogen transformation processes such as nitrification and denitrification. Soils represent the largest sources of N(2)O emissions with nitrogen fertilizer application being the main driver of rising atmospheric N(2)...

Descripción completa

Detalles Bibliográficos
Autores principales: Harter, Johannes, Guzman-Bustamante, Ivan, Kuehfuss, Stefanie, Ruser, Reiner, Well, Reinhard, Spott, Oliver, Kappler, Andreas, Behrens, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5180216/
https://www.ncbi.nlm.nih.gov/pubmed/28008997
http://dx.doi.org/10.1038/srep39574
_version_ 1782485486613299200
author Harter, Johannes
Guzman-Bustamante, Ivan
Kuehfuss, Stefanie
Ruser, Reiner
Well, Reinhard
Spott, Oliver
Kappler, Andreas
Behrens, Sebastian
author_facet Harter, Johannes
Guzman-Bustamante, Ivan
Kuehfuss, Stefanie
Ruser, Reiner
Well, Reinhard
Spott, Oliver
Kappler, Andreas
Behrens, Sebastian
author_sort Harter, Johannes
collection PubMed
description Nitrous oxide (N(2)O) is a potent greenhouse gas that is produced during microbial nitrogen transformation processes such as nitrification and denitrification. Soils represent the largest sources of N(2)O emissions with nitrogen fertilizer application being the main driver of rising atmospheric N(2)O concentrations. Soil biochar amendment has been proposed as a promising tool to mitigate N(2)O emissions from soils. However, the underlying processes that cause N(2)O emission suppression in biochar-amended soils are still poorly understood. We set up microcosm experiments with fertilized, wet soil in which we used (15)N tracing techniques and quantitative polymerase chain reaction (qPCR) to investigate the impact of biochar on mineral and gaseous nitrogen dynamics and denitrification-specific functional marker gene abundance and expression. In accordance with previous studies our results showed that biochar addition can lead to a significant decrease in N(2)O emissions. Furthermore, we determined significantly higher quantities of soil-entrapped N(2)O and N(2) in biochar microcosms and a biochar-induced increase in typical and atypical nosZ transcript copy numbers. Our findings suggest that biochar-induced N(2)O emission mitigation is based on the entrapment of N(2)O in water-saturated pores of the soil matrix and concurrent stimulation of microbial N(2)O reduction resulting in an overall decrease of the N(2)O/(N(2)O + N(2)) ratio.
format Online
Article
Text
id pubmed-5180216
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-51802162016-12-29 Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil Harter, Johannes Guzman-Bustamante, Ivan Kuehfuss, Stefanie Ruser, Reiner Well, Reinhard Spott, Oliver Kappler, Andreas Behrens, Sebastian Sci Rep Article Nitrous oxide (N(2)O) is a potent greenhouse gas that is produced during microbial nitrogen transformation processes such as nitrification and denitrification. Soils represent the largest sources of N(2)O emissions with nitrogen fertilizer application being the main driver of rising atmospheric N(2)O concentrations. Soil biochar amendment has been proposed as a promising tool to mitigate N(2)O emissions from soils. However, the underlying processes that cause N(2)O emission suppression in biochar-amended soils are still poorly understood. We set up microcosm experiments with fertilized, wet soil in which we used (15)N tracing techniques and quantitative polymerase chain reaction (qPCR) to investigate the impact of biochar on mineral and gaseous nitrogen dynamics and denitrification-specific functional marker gene abundance and expression. In accordance with previous studies our results showed that biochar addition can lead to a significant decrease in N(2)O emissions. Furthermore, we determined significantly higher quantities of soil-entrapped N(2)O and N(2) in biochar microcosms and a biochar-induced increase in typical and atypical nosZ transcript copy numbers. Our findings suggest that biochar-induced N(2)O emission mitigation is based on the entrapment of N(2)O in water-saturated pores of the soil matrix and concurrent stimulation of microbial N(2)O reduction resulting in an overall decrease of the N(2)O/(N(2)O + N(2)) ratio. Nature Publishing Group 2016-12-23 /pmc/articles/PMC5180216/ /pubmed/28008997 http://dx.doi.org/10.1038/srep39574 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Harter, Johannes
Guzman-Bustamante, Ivan
Kuehfuss, Stefanie
Ruser, Reiner
Well, Reinhard
Spott, Oliver
Kappler, Andreas
Behrens, Sebastian
Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil
title Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil
title_full Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil
title_fullStr Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil
title_full_unstemmed Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil
title_short Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil
title_sort gas entrapment and microbial n(2)o reduction reduce n(2)o emissions from a biochar-amended sandy clay loam soil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5180216/
https://www.ncbi.nlm.nih.gov/pubmed/28008997
http://dx.doi.org/10.1038/srep39574
work_keys_str_mv AT harterjohannes gasentrapmentandmicrobialn2oreductionreducen2oemissionsfromabiocharamendedsandyclayloamsoil
AT guzmanbustamanteivan gasentrapmentandmicrobialn2oreductionreducen2oemissionsfromabiocharamendedsandyclayloamsoil
AT kuehfussstefanie gasentrapmentandmicrobialn2oreductionreducen2oemissionsfromabiocharamendedsandyclayloamsoil
AT ruserreiner gasentrapmentandmicrobialn2oreductionreducen2oemissionsfromabiocharamendedsandyclayloamsoil
AT wellreinhard gasentrapmentandmicrobialn2oreductionreducen2oemissionsfromabiocharamendedsandyclayloamsoil
AT spottoliver gasentrapmentandmicrobialn2oreductionreducen2oemissionsfromabiocharamendedsandyclayloamsoil
AT kapplerandreas gasentrapmentandmicrobialn2oreductionreducen2oemissionsfromabiocharamendedsandyclayloamsoil
AT behrenssebastian gasentrapmentandmicrobialn2oreductionreducen2oemissionsfromabiocharamendedsandyclayloamsoil