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Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil
Nitrous oxide (N(2)O) is a potent greenhouse gas that is produced during microbial nitrogen transformation processes such as nitrification and denitrification. Soils represent the largest sources of N(2)O emissions with nitrogen fertilizer application being the main driver of rising atmospheric N(2)...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5180216/ https://www.ncbi.nlm.nih.gov/pubmed/28008997 http://dx.doi.org/10.1038/srep39574 |
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author | Harter, Johannes Guzman-Bustamante, Ivan Kuehfuss, Stefanie Ruser, Reiner Well, Reinhard Spott, Oliver Kappler, Andreas Behrens, Sebastian |
author_facet | Harter, Johannes Guzman-Bustamante, Ivan Kuehfuss, Stefanie Ruser, Reiner Well, Reinhard Spott, Oliver Kappler, Andreas Behrens, Sebastian |
author_sort | Harter, Johannes |
collection | PubMed |
description | Nitrous oxide (N(2)O) is a potent greenhouse gas that is produced during microbial nitrogen transformation processes such as nitrification and denitrification. Soils represent the largest sources of N(2)O emissions with nitrogen fertilizer application being the main driver of rising atmospheric N(2)O concentrations. Soil biochar amendment has been proposed as a promising tool to mitigate N(2)O emissions from soils. However, the underlying processes that cause N(2)O emission suppression in biochar-amended soils are still poorly understood. We set up microcosm experiments with fertilized, wet soil in which we used (15)N tracing techniques and quantitative polymerase chain reaction (qPCR) to investigate the impact of biochar on mineral and gaseous nitrogen dynamics and denitrification-specific functional marker gene abundance and expression. In accordance with previous studies our results showed that biochar addition can lead to a significant decrease in N(2)O emissions. Furthermore, we determined significantly higher quantities of soil-entrapped N(2)O and N(2) in biochar microcosms and a biochar-induced increase in typical and atypical nosZ transcript copy numbers. Our findings suggest that biochar-induced N(2)O emission mitigation is based on the entrapment of N(2)O in water-saturated pores of the soil matrix and concurrent stimulation of microbial N(2)O reduction resulting in an overall decrease of the N(2)O/(N(2)O + N(2)) ratio. |
format | Online Article Text |
id | pubmed-5180216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51802162016-12-29 Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil Harter, Johannes Guzman-Bustamante, Ivan Kuehfuss, Stefanie Ruser, Reiner Well, Reinhard Spott, Oliver Kappler, Andreas Behrens, Sebastian Sci Rep Article Nitrous oxide (N(2)O) is a potent greenhouse gas that is produced during microbial nitrogen transformation processes such as nitrification and denitrification. Soils represent the largest sources of N(2)O emissions with nitrogen fertilizer application being the main driver of rising atmospheric N(2)O concentrations. Soil biochar amendment has been proposed as a promising tool to mitigate N(2)O emissions from soils. However, the underlying processes that cause N(2)O emission suppression in biochar-amended soils are still poorly understood. We set up microcosm experiments with fertilized, wet soil in which we used (15)N tracing techniques and quantitative polymerase chain reaction (qPCR) to investigate the impact of biochar on mineral and gaseous nitrogen dynamics and denitrification-specific functional marker gene abundance and expression. In accordance with previous studies our results showed that biochar addition can lead to a significant decrease in N(2)O emissions. Furthermore, we determined significantly higher quantities of soil-entrapped N(2)O and N(2) in biochar microcosms and a biochar-induced increase in typical and atypical nosZ transcript copy numbers. Our findings suggest that biochar-induced N(2)O emission mitigation is based on the entrapment of N(2)O in water-saturated pores of the soil matrix and concurrent stimulation of microbial N(2)O reduction resulting in an overall decrease of the N(2)O/(N(2)O + N(2)) ratio. Nature Publishing Group 2016-12-23 /pmc/articles/PMC5180216/ /pubmed/28008997 http://dx.doi.org/10.1038/srep39574 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Harter, Johannes Guzman-Bustamante, Ivan Kuehfuss, Stefanie Ruser, Reiner Well, Reinhard Spott, Oliver Kappler, Andreas Behrens, Sebastian Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil |
title | Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil |
title_full | Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil |
title_fullStr | Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil |
title_full_unstemmed | Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil |
title_short | Gas entrapment and microbial N(2)O reduction reduce N(2)O emissions from a biochar-amended sandy clay loam soil |
title_sort | gas entrapment and microbial n(2)o reduction reduce n(2)o emissions from a biochar-amended sandy clay loam soil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5180216/ https://www.ncbi.nlm.nih.gov/pubmed/28008997 http://dx.doi.org/10.1038/srep39574 |
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