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The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance
Campylobacter jejuni is the leading cause of bacterial foodborne diarrhoeal disease worldwide. Despite the microaerophilic nature of the bacterium, C. jejuni can survive the atmospheric oxygen conditions in the environment. Bacteria that can survive either within a host or in the environment like C....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5183652/ https://www.ncbi.nlm.nih.gov/pubmed/28082970 http://dx.doi.org/10.3389/fmicb.2016.02117 |
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author | Gundogdu, Ozan da Silva, Daiani T. Mohammad, Banaz Elmi, Abdi Wren, Brendan W. van Vliet, Arnoud H. M. Dorrell, Nick |
author_facet | Gundogdu, Ozan da Silva, Daiani T. Mohammad, Banaz Elmi, Abdi Wren, Brendan W. van Vliet, Arnoud H. M. Dorrell, Nick |
author_sort | Gundogdu, Ozan |
collection | PubMed |
description | Campylobacter jejuni is the leading cause of bacterial foodborne diarrhoeal disease worldwide. Despite the microaerophilic nature of the bacterium, C. jejuni can survive the atmospheric oxygen conditions in the environment. Bacteria that can survive either within a host or in the environment like C. jejuni require variable responses to survive the stresses associated with exposure to different levels of reactive oxygen species. The MarR-type transcriptional regulators RrpA and RrpB have recently been shown to play a role in controlling both the C. jejuni oxidative and aerobic stress responses. Analysis of 3,746 C. jejuni and 486 C. coli genome sequences showed that whilst rrpA is present in over 99% of C. jejuni strains, the presence of rrpB is restricted and appears to correlate with specific MLST clonal complexes (predominantly ST-21 and ST-61). C. coli strains in contrast lack both rrpA and rrpB. In C. jejuni rrpB(+) strains, the rrpB gene is located within a variable genomic region containing the IF subtype of the type I Restriction-Modification (hsd) system, whilst this variable genomic region in C. jejuni rrpB(-) strains contains the IAB subtype hsd system and not the rrpB gene. C. jejuni rrpB(-) strains exhibit greater resistance to peroxide and aerobic stress than C. jejuni rrpB(+) strains. Inactivation of rrpA resulted in increased sensitivity to peroxide stress in rrpB(+) strains, but not in rrpB(-) strains. Mutation of rrpA resulted in reduced killing of Galleria mellonella larvae and enhanced biofilm formation independent of rrpB status. The oxidative and aerobic stress responses of rrpB(-) and rrpB(+) strains suggest adaptation of C. jejuni within different hosts and niches that can be linked to specific MLST clonal complexes. |
format | Online Article Text |
id | pubmed-5183652 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51836522017-01-12 The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance Gundogdu, Ozan da Silva, Daiani T. Mohammad, Banaz Elmi, Abdi Wren, Brendan W. van Vliet, Arnoud H. M. Dorrell, Nick Front Microbiol Microbiology Campylobacter jejuni is the leading cause of bacterial foodborne diarrhoeal disease worldwide. Despite the microaerophilic nature of the bacterium, C. jejuni can survive the atmospheric oxygen conditions in the environment. Bacteria that can survive either within a host or in the environment like C. jejuni require variable responses to survive the stresses associated with exposure to different levels of reactive oxygen species. The MarR-type transcriptional regulators RrpA and RrpB have recently been shown to play a role in controlling both the C. jejuni oxidative and aerobic stress responses. Analysis of 3,746 C. jejuni and 486 C. coli genome sequences showed that whilst rrpA is present in over 99% of C. jejuni strains, the presence of rrpB is restricted and appears to correlate with specific MLST clonal complexes (predominantly ST-21 and ST-61). C. coli strains in contrast lack both rrpA and rrpB. In C. jejuni rrpB(+) strains, the rrpB gene is located within a variable genomic region containing the IF subtype of the type I Restriction-Modification (hsd) system, whilst this variable genomic region in C. jejuni rrpB(-) strains contains the IAB subtype hsd system and not the rrpB gene. C. jejuni rrpB(-) strains exhibit greater resistance to peroxide and aerobic stress than C. jejuni rrpB(+) strains. Inactivation of rrpA resulted in increased sensitivity to peroxide stress in rrpB(+) strains, but not in rrpB(-) strains. Mutation of rrpA resulted in reduced killing of Galleria mellonella larvae and enhanced biofilm formation independent of rrpB status. The oxidative and aerobic stress responses of rrpB(-) and rrpB(+) strains suggest adaptation of C. jejuni within different hosts and niches that can be linked to specific MLST clonal complexes. Frontiers Media S.A. 2016-12-26 /pmc/articles/PMC5183652/ /pubmed/28082970 http://dx.doi.org/10.3389/fmicb.2016.02117 Text en Copyright © 2016 Gundogdu, da Silva, Mohammad, Elmi, Wren, van Vliet and Dorrell. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Gundogdu, Ozan da Silva, Daiani T. Mohammad, Banaz Elmi, Abdi Wren, Brendan W. van Vliet, Arnoud H. M. Dorrell, Nick The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance |
title | The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance |
title_full | The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance |
title_fullStr | The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance |
title_full_unstemmed | The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance |
title_short | The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance |
title_sort | campylobacter jejuni oxidative stress regulator rrpb is associated with a genomic hypervariable region and altered oxidative stress resistance |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5183652/ https://www.ncbi.nlm.nih.gov/pubmed/28082970 http://dx.doi.org/10.3389/fmicb.2016.02117 |
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