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The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance

Campylobacter jejuni is the leading cause of bacterial foodborne diarrhoeal disease worldwide. Despite the microaerophilic nature of the bacterium, C. jejuni can survive the atmospheric oxygen conditions in the environment. Bacteria that can survive either within a host or in the environment like C....

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Autores principales: Gundogdu, Ozan, da Silva, Daiani T., Mohammad, Banaz, Elmi, Abdi, Wren, Brendan W., van Vliet, Arnoud H. M., Dorrell, Nick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5183652/
https://www.ncbi.nlm.nih.gov/pubmed/28082970
http://dx.doi.org/10.3389/fmicb.2016.02117
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author Gundogdu, Ozan
da Silva, Daiani T.
Mohammad, Banaz
Elmi, Abdi
Wren, Brendan W.
van Vliet, Arnoud H. M.
Dorrell, Nick
author_facet Gundogdu, Ozan
da Silva, Daiani T.
Mohammad, Banaz
Elmi, Abdi
Wren, Brendan W.
van Vliet, Arnoud H. M.
Dorrell, Nick
author_sort Gundogdu, Ozan
collection PubMed
description Campylobacter jejuni is the leading cause of bacterial foodborne diarrhoeal disease worldwide. Despite the microaerophilic nature of the bacterium, C. jejuni can survive the atmospheric oxygen conditions in the environment. Bacteria that can survive either within a host or in the environment like C. jejuni require variable responses to survive the stresses associated with exposure to different levels of reactive oxygen species. The MarR-type transcriptional regulators RrpA and RrpB have recently been shown to play a role in controlling both the C. jejuni oxidative and aerobic stress responses. Analysis of 3,746 C. jejuni and 486 C. coli genome sequences showed that whilst rrpA is present in over 99% of C. jejuni strains, the presence of rrpB is restricted and appears to correlate with specific MLST clonal complexes (predominantly ST-21 and ST-61). C. coli strains in contrast lack both rrpA and rrpB. In C. jejuni rrpB(+) strains, the rrpB gene is located within a variable genomic region containing the IF subtype of the type I Restriction-Modification (hsd) system, whilst this variable genomic region in C. jejuni rrpB(-) strains contains the IAB subtype hsd system and not the rrpB gene. C. jejuni rrpB(-) strains exhibit greater resistance to peroxide and aerobic stress than C. jejuni rrpB(+) strains. Inactivation of rrpA resulted in increased sensitivity to peroxide stress in rrpB(+) strains, but not in rrpB(-) strains. Mutation of rrpA resulted in reduced killing of Galleria mellonella larvae and enhanced biofilm formation independent of rrpB status. The oxidative and aerobic stress responses of rrpB(-) and rrpB(+) strains suggest adaptation of C. jejuni within different hosts and niches that can be linked to specific MLST clonal complexes.
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spelling pubmed-51836522017-01-12 The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance Gundogdu, Ozan da Silva, Daiani T. Mohammad, Banaz Elmi, Abdi Wren, Brendan W. van Vliet, Arnoud H. M. Dorrell, Nick Front Microbiol Microbiology Campylobacter jejuni is the leading cause of bacterial foodborne diarrhoeal disease worldwide. Despite the microaerophilic nature of the bacterium, C. jejuni can survive the atmospheric oxygen conditions in the environment. Bacteria that can survive either within a host or in the environment like C. jejuni require variable responses to survive the stresses associated with exposure to different levels of reactive oxygen species. The MarR-type transcriptional regulators RrpA and RrpB have recently been shown to play a role in controlling both the C. jejuni oxidative and aerobic stress responses. Analysis of 3,746 C. jejuni and 486 C. coli genome sequences showed that whilst rrpA is present in over 99% of C. jejuni strains, the presence of rrpB is restricted and appears to correlate with specific MLST clonal complexes (predominantly ST-21 and ST-61). C. coli strains in contrast lack both rrpA and rrpB. In C. jejuni rrpB(+) strains, the rrpB gene is located within a variable genomic region containing the IF subtype of the type I Restriction-Modification (hsd) system, whilst this variable genomic region in C. jejuni rrpB(-) strains contains the IAB subtype hsd system and not the rrpB gene. C. jejuni rrpB(-) strains exhibit greater resistance to peroxide and aerobic stress than C. jejuni rrpB(+) strains. Inactivation of rrpA resulted in increased sensitivity to peroxide stress in rrpB(+) strains, but not in rrpB(-) strains. Mutation of rrpA resulted in reduced killing of Galleria mellonella larvae and enhanced biofilm formation independent of rrpB status. The oxidative and aerobic stress responses of rrpB(-) and rrpB(+) strains suggest adaptation of C. jejuni within different hosts and niches that can be linked to specific MLST clonal complexes. Frontiers Media S.A. 2016-12-26 /pmc/articles/PMC5183652/ /pubmed/28082970 http://dx.doi.org/10.3389/fmicb.2016.02117 Text en Copyright © 2016 Gundogdu, da Silva, Mohammad, Elmi, Wren, van Vliet and Dorrell. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gundogdu, Ozan
da Silva, Daiani T.
Mohammad, Banaz
Elmi, Abdi
Wren, Brendan W.
van Vliet, Arnoud H. M.
Dorrell, Nick
The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance
title The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance
title_full The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance
title_fullStr The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance
title_full_unstemmed The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance
title_short The Campylobacter jejuni Oxidative Stress Regulator RrpB Is Associated with a Genomic Hypervariable Region and Altered Oxidative Stress Resistance
title_sort campylobacter jejuni oxidative stress regulator rrpb is associated with a genomic hypervariable region and altered oxidative stress resistance
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5183652/
https://www.ncbi.nlm.nih.gov/pubmed/28082970
http://dx.doi.org/10.3389/fmicb.2016.02117
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