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11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet

The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) interconverts active glucocorticoids and their intrinsically inert 11-keto forms. The type 1 isozyme, 11β-HSD1, predominantly reactivates glucocorticoids in vivo and can also metabolise bile acids. 11β-HSD1-deficient mice show altered inflammator...

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Autores principales: Johnson, Jethro S, Opiyo, Monica N, Thomson, Marian, Gharbi, Karim, Seckl, Jonathan R, Heger, Andreas, Chapman, Karen E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5184774/
https://www.ncbi.nlm.nih.gov/pubmed/27885053
http://dx.doi.org/10.1530/JOE-16-0578
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author Johnson, Jethro S
Opiyo, Monica N
Thomson, Marian
Gharbi, Karim
Seckl, Jonathan R
Heger, Andreas
Chapman, Karen E
author_facet Johnson, Jethro S
Opiyo, Monica N
Thomson, Marian
Gharbi, Karim
Seckl, Jonathan R
Heger, Andreas
Chapman, Karen E
author_sort Johnson, Jethro S
collection PubMed
description The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) interconverts active glucocorticoids and their intrinsically inert 11-keto forms. The type 1 isozyme, 11β-HSD1, predominantly reactivates glucocorticoids in vivo and can also metabolise bile acids. 11β-HSD1-deficient mice show altered inflammatory responses and are protected against the adverse metabolic effects of a high-fat diet. However, the impact of 11β-HSD1 on the composition of the gut microbiome has not previously been investigated. We used high-throughput 16S rDNA amplicon sequencing to characterise the gut microbiome of 11β-HSD1-deficient and C57Bl/6 control mice, fed either a standard chow diet or a cholesterol- and fat-enriched ‘Western’ diet. 11β-HSD1 deficiency significantly altered the composition of the gut microbiome, and did so in a diet-specific manner. On a Western diet, 11β-HSD1 deficiency increased the relative abundance of the family Bacteroidaceae, and on a chow diet, it altered relative abundance of the family Prevotellaceae. Our results demonstrate that (i) genetic effects on host–microbiome interactions can depend upon diet and (ii) that alterations in the composition of the gut microbiome may contribute to the aspects of the metabolic and/or inflammatory phenotype observed with 11β-HSD1 deficiency.
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spelling pubmed-51847742017-01-04 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet Johnson, Jethro S Opiyo, Monica N Thomson, Marian Gharbi, Karim Seckl, Jonathan R Heger, Andreas Chapman, Karen E J Endocrinol Research The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) interconverts active glucocorticoids and their intrinsically inert 11-keto forms. The type 1 isozyme, 11β-HSD1, predominantly reactivates glucocorticoids in vivo and can also metabolise bile acids. 11β-HSD1-deficient mice show altered inflammatory responses and are protected against the adverse metabolic effects of a high-fat diet. However, the impact of 11β-HSD1 on the composition of the gut microbiome has not previously been investigated. We used high-throughput 16S rDNA amplicon sequencing to characterise the gut microbiome of 11β-HSD1-deficient and C57Bl/6 control mice, fed either a standard chow diet or a cholesterol- and fat-enriched ‘Western’ diet. 11β-HSD1 deficiency significantly altered the composition of the gut microbiome, and did so in a diet-specific manner. On a Western diet, 11β-HSD1 deficiency increased the relative abundance of the family Bacteroidaceae, and on a chow diet, it altered relative abundance of the family Prevotellaceae. Our results demonstrate that (i) genetic effects on host–microbiome interactions can depend upon diet and (ii) that alterations in the composition of the gut microbiome may contribute to the aspects of the metabolic and/or inflammatory phenotype observed with 11β-HSD1 deficiency. Bioscientifica Ltd 2016-12-16 /pmc/articles/PMC5184774/ /pubmed/27885053 http://dx.doi.org/10.1530/JOE-16-0578 Text en © 2017 The authors http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Research
Johnson, Jethro S
Opiyo, Monica N
Thomson, Marian
Gharbi, Karim
Seckl, Jonathan R
Heger, Andreas
Chapman, Karen E
11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet
title 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet
title_full 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet
title_fullStr 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet
title_full_unstemmed 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet
title_short 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet
title_sort 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to western diet
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5184774/
https://www.ncbi.nlm.nih.gov/pubmed/27885053
http://dx.doi.org/10.1530/JOE-16-0578
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