Cargando…
Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5186903/ https://www.ncbi.nlm.nih.gov/pubmed/27974214 http://dx.doi.org/10.1016/j.celrep.2016.11.049 |
_version_ | 1782486731717607424 |
---|---|
author | Glytsou, Christina Calvo, Enrique Cogliati, Sara Mehrotra, Arpit Anastasia, Irene Rigoni, Giovanni Raimondi, Andrea Shintani, Norihito Loureiro, Marta Vazquez, Jesùs Pellegrini, Luca Enriquez, Jose Antonio Scorrano, Luca Soriano, Maria Eugenia |
author_facet | Glytsou, Christina Calvo, Enrique Cogliati, Sara Mehrotra, Arpit Anastasia, Irene Rigoni, Giovanni Raimondi, Andrea Shintani, Norihito Loureiro, Marta Vazquez, Jesùs Pellegrini, Luca Enriquez, Jose Antonio Scorrano, Luca Soriano, Maria Eugenia |
author_sort | Glytsou, Christina |
collection | PubMed |
description | The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS. |
format | Online Article Text |
id | pubmed-5186903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-51869032017-01-03 Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control Glytsou, Christina Calvo, Enrique Cogliati, Sara Mehrotra, Arpit Anastasia, Irene Rigoni, Giovanni Raimondi, Andrea Shintani, Norihito Loureiro, Marta Vazquez, Jesùs Pellegrini, Luca Enriquez, Jose Antonio Scorrano, Luca Soriano, Maria Eugenia Cell Rep Article The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS. Cell Press 2016-12-13 /pmc/articles/PMC5186903/ /pubmed/27974214 http://dx.doi.org/10.1016/j.celrep.2016.11.049 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Glytsou, Christina Calvo, Enrique Cogliati, Sara Mehrotra, Arpit Anastasia, Irene Rigoni, Giovanni Raimondi, Andrea Shintani, Norihito Loureiro, Marta Vazquez, Jesùs Pellegrini, Luca Enriquez, Jose Antonio Scorrano, Luca Soriano, Maria Eugenia Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control |
title | Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control |
title_full | Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control |
title_fullStr | Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control |
title_full_unstemmed | Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control |
title_short | Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control |
title_sort | optic atrophy 1 is epistatic to the core micos component mic60 in mitochondrial cristae shape control |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5186903/ https://www.ncbi.nlm.nih.gov/pubmed/27974214 http://dx.doi.org/10.1016/j.celrep.2016.11.049 |
work_keys_str_mv | AT glytsouchristina opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT calvoenrique opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT cogliatisara opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT mehrotraarpit opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT anastasiairene opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT rigonigiovanni opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT raimondiandrea opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT shintaninorihito opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT loureiromarta opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT vazquezjesus opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT pellegriniluca opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT enriquezjoseantonio opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT scorranoluca opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol AT sorianomariaeugenia opticatrophy1isepistatictothecoremicoscomponentmic60inmitochondrialcristaeshapecontrol |