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Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control

The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS...

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Autores principales: Glytsou, Christina, Calvo, Enrique, Cogliati, Sara, Mehrotra, Arpit, Anastasia, Irene, Rigoni, Giovanni, Raimondi, Andrea, Shintani, Norihito, Loureiro, Marta, Vazquez, Jesùs, Pellegrini, Luca, Enriquez, Jose Antonio, Scorrano, Luca, Soriano, Maria Eugenia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5186903/
https://www.ncbi.nlm.nih.gov/pubmed/27974214
http://dx.doi.org/10.1016/j.celrep.2016.11.049
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author Glytsou, Christina
Calvo, Enrique
Cogliati, Sara
Mehrotra, Arpit
Anastasia, Irene
Rigoni, Giovanni
Raimondi, Andrea
Shintani, Norihito
Loureiro, Marta
Vazquez, Jesùs
Pellegrini, Luca
Enriquez, Jose Antonio
Scorrano, Luca
Soriano, Maria Eugenia
author_facet Glytsou, Christina
Calvo, Enrique
Cogliati, Sara
Mehrotra, Arpit
Anastasia, Irene
Rigoni, Giovanni
Raimondi, Andrea
Shintani, Norihito
Loureiro, Marta
Vazquez, Jesùs
Pellegrini, Luca
Enriquez, Jose Antonio
Scorrano, Luca
Soriano, Maria Eugenia
author_sort Glytsou, Christina
collection PubMed
description The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS.
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spelling pubmed-51869032017-01-03 Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control Glytsou, Christina Calvo, Enrique Cogliati, Sara Mehrotra, Arpit Anastasia, Irene Rigoni, Giovanni Raimondi, Andrea Shintani, Norihito Loureiro, Marta Vazquez, Jesùs Pellegrini, Luca Enriquez, Jose Antonio Scorrano, Luca Soriano, Maria Eugenia Cell Rep Article The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS. Cell Press 2016-12-13 /pmc/articles/PMC5186903/ /pubmed/27974214 http://dx.doi.org/10.1016/j.celrep.2016.11.049 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Glytsou, Christina
Calvo, Enrique
Cogliati, Sara
Mehrotra, Arpit
Anastasia, Irene
Rigoni, Giovanni
Raimondi, Andrea
Shintani, Norihito
Loureiro, Marta
Vazquez, Jesùs
Pellegrini, Luca
Enriquez, Jose Antonio
Scorrano, Luca
Soriano, Maria Eugenia
Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title_full Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title_fullStr Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title_full_unstemmed Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title_short Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title_sort optic atrophy 1 is epistatic to the core micos component mic60 in mitochondrial cristae shape control
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5186903/
https://www.ncbi.nlm.nih.gov/pubmed/27974214
http://dx.doi.org/10.1016/j.celrep.2016.11.049
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