Cargando…

Non-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors

Rapid development of plants is important for the production of ‘baby-leaf’ lettuce that is harvested when plants reach the four- to eight-leaf stage of growth. However, environmental factors, such as high or low temperature, or elevated concentrations of salt, inhibit lettuce growth. Therefore, non-...

Descripción completa

Detalles Bibliográficos
Autores principales: Simko, Ivan, Hayes, Ryan J., Furbank, Robert T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187177/
https://www.ncbi.nlm.nih.gov/pubmed/28083011
http://dx.doi.org/10.3389/fpls.2016.01985
_version_ 1782486788435083264
author Simko, Ivan
Hayes, Ryan J.
Furbank, Robert T.
author_facet Simko, Ivan
Hayes, Ryan J.
Furbank, Robert T.
author_sort Simko, Ivan
collection PubMed
description Rapid development of plants is important for the production of ‘baby-leaf’ lettuce that is harvested when plants reach the four- to eight-leaf stage of growth. However, environmental factors, such as high or low temperature, or elevated concentrations of salt, inhibit lettuce growth. Therefore, non-destructive evaluations of plants can provide valuable information to breeders and growers. The objective of the present study was to test the feasibility of using non-destructive phenotyping with optical sensors for the evaluations of lettuce plants in early stages of development. We performed the series of experiments to determine if hyperspectral imaging and chlorophyll fluorescence imaging can determine phenotypic changes manifested on lettuce plants subjected to the extreme temperature and salinity stress treatments. Our results indicate that top view optical sensors alone can accurately determine plant size to approximately 7 g fresh weight. Hyperspectral imaging analysis was able to detect changes in the total chlorophyll (RCC) and anthocyanin (RAC) content, while chlorophyll fluorescence imaging revealed photoinhibition and reduction of plant growth caused by the extreme growing temperatures (3 and 39°C) and salinity (100 mM NaCl). Though no significant correlation was found between F(v)/F(m) and decrease in plant growth due to stress when comparisons were made across multiple accessions, our results indicate that lettuce plants have a high adaptability to both low (3°C) and high (39°C) temperatures, with no permanent damage to photosynthetic apparatus and fast recovery of plants after moving them to the optimal (21°C) temperature. We have also detected a strong relationship between visual rating of the green- and red-leaf color intensity and RCC and RAC, respectively. Differences in RAC among accessions suggest that the selection for intense red color may be easier to perform at somewhat lower than the optimal temperature. This study serves as a proof of concept that optical sensors can be successfully used as tools for breeders when evaluating young lettuce plants. Moreover, we were able to identify the locus for light green leaf color (qLG4), and position this locus on the molecular linkage map of lettuce, which shows that these techniques have sufficient resolution to be used in a genetic context in lettuce.
format Online
Article
Text
id pubmed-5187177
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-51871772017-01-12 Non-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors Simko, Ivan Hayes, Ryan J. Furbank, Robert T. Front Plant Sci Plant Science Rapid development of plants is important for the production of ‘baby-leaf’ lettuce that is harvested when plants reach the four- to eight-leaf stage of growth. However, environmental factors, such as high or low temperature, or elevated concentrations of salt, inhibit lettuce growth. Therefore, non-destructive evaluations of plants can provide valuable information to breeders and growers. The objective of the present study was to test the feasibility of using non-destructive phenotyping with optical sensors for the evaluations of lettuce plants in early stages of development. We performed the series of experiments to determine if hyperspectral imaging and chlorophyll fluorescence imaging can determine phenotypic changes manifested on lettuce plants subjected to the extreme temperature and salinity stress treatments. Our results indicate that top view optical sensors alone can accurately determine plant size to approximately 7 g fresh weight. Hyperspectral imaging analysis was able to detect changes in the total chlorophyll (RCC) and anthocyanin (RAC) content, while chlorophyll fluorescence imaging revealed photoinhibition and reduction of plant growth caused by the extreme growing temperatures (3 and 39°C) and salinity (100 mM NaCl). Though no significant correlation was found between F(v)/F(m) and decrease in plant growth due to stress when comparisons were made across multiple accessions, our results indicate that lettuce plants have a high adaptability to both low (3°C) and high (39°C) temperatures, with no permanent damage to photosynthetic apparatus and fast recovery of plants after moving them to the optimal (21°C) temperature. We have also detected a strong relationship between visual rating of the green- and red-leaf color intensity and RCC and RAC, respectively. Differences in RAC among accessions suggest that the selection for intense red color may be easier to perform at somewhat lower than the optimal temperature. This study serves as a proof of concept that optical sensors can be successfully used as tools for breeders when evaluating young lettuce plants. Moreover, we were able to identify the locus for light green leaf color (qLG4), and position this locus on the molecular linkage map of lettuce, which shows that these techniques have sufficient resolution to be used in a genetic context in lettuce. Frontiers Media S.A. 2016-12-27 /pmc/articles/PMC5187177/ /pubmed/28083011 http://dx.doi.org/10.3389/fpls.2016.01985 Text en Copyright © 2016 Simko, Hayes and Furbank. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Simko, Ivan
Hayes, Ryan J.
Furbank, Robert T.
Non-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors
title Non-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors
title_full Non-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors
title_fullStr Non-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors
title_full_unstemmed Non-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors
title_short Non-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors
title_sort non-destructive phenotyping of lettuce plants in early stages of development with optical sensors
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187177/
https://www.ncbi.nlm.nih.gov/pubmed/28083011
http://dx.doi.org/10.3389/fpls.2016.01985
work_keys_str_mv AT simkoivan nondestructivephenotypingoflettuceplantsinearlystagesofdevelopmentwithopticalsensors
AT hayesryanj nondestructivephenotypingoflettuceplantsinearlystagesofdevelopmentwithopticalsensors
AT furbankrobertt nondestructivephenotypingoflettuceplantsinearlystagesofdevelopmentwithopticalsensors