Cargando…
Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers
Promising targeted therapy and personalized medicine are making molecular profiling of tumours a priority. For colorectal cancer (CRC) patients, international guidelines made RAS (KRAS and NRAS) status a prerequisite for the use of anti-epidermal growth factor receptor agents (anti-EGFR). Daily, new...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187917/ https://www.ncbi.nlm.nih.gov/pubmed/27999270 http://dx.doi.org/10.3390/ijms17122117 |
_version_ | 1782486926926807040 |
---|---|
author | Fontanges, Quitterie De Mendonca, Ricardo Salmon, Isabelle Le Mercier, Marie D’Haene, Nicky |
author_facet | Fontanges, Quitterie De Mendonca, Ricardo Salmon, Isabelle Le Mercier, Marie D’Haene, Nicky |
author_sort | Fontanges, Quitterie |
collection | PubMed |
description | Promising targeted therapy and personalized medicine are making molecular profiling of tumours a priority. For colorectal cancer (CRC) patients, international guidelines made RAS (KRAS and NRAS) status a prerequisite for the use of anti-epidermal growth factor receptor agents (anti-EGFR). Daily, new data emerge on the theranostic and prognostic role of molecular biomarkers, which is a strong incentive for a validated, sensitive and broadly available molecular screening test in order to implement and improve multi-modal therapy strategy and clinical trials. Next generation sequencing (NGS) has begun to supplant other technologies for genomic profiling. Targeted NGS is a method that allows parallel sequencing of thousands of short DNA sequences in a single test offering a cost-effective approach for detecting multiple genetic alterations with a minimum amount of DNA. In the present review, we collected data concerning the clinical application of NGS technology in the setting of colorectal cancer. |
format | Online Article Text |
id | pubmed-5187917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-51879172016-12-30 Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers Fontanges, Quitterie De Mendonca, Ricardo Salmon, Isabelle Le Mercier, Marie D’Haene, Nicky Int J Mol Sci Review Promising targeted therapy and personalized medicine are making molecular profiling of tumours a priority. For colorectal cancer (CRC) patients, international guidelines made RAS (KRAS and NRAS) status a prerequisite for the use of anti-epidermal growth factor receptor agents (anti-EGFR). Daily, new data emerge on the theranostic and prognostic role of molecular biomarkers, which is a strong incentive for a validated, sensitive and broadly available molecular screening test in order to implement and improve multi-modal therapy strategy and clinical trials. Next generation sequencing (NGS) has begun to supplant other technologies for genomic profiling. Targeted NGS is a method that allows parallel sequencing of thousands of short DNA sequences in a single test offering a cost-effective approach for detecting multiple genetic alterations with a minimum amount of DNA. In the present review, we collected data concerning the clinical application of NGS technology in the setting of colorectal cancer. MDPI 2016-12-16 /pmc/articles/PMC5187917/ /pubmed/27999270 http://dx.doi.org/10.3390/ijms17122117 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Fontanges, Quitterie De Mendonca, Ricardo Salmon, Isabelle Le Mercier, Marie D’Haene, Nicky Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers |
title | Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers |
title_full | Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers |
title_fullStr | Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers |
title_full_unstemmed | Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers |
title_short | Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers |
title_sort | clinical application of targeted next generation sequencing for colorectal cancers |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187917/ https://www.ncbi.nlm.nih.gov/pubmed/27999270 http://dx.doi.org/10.3390/ijms17122117 |
work_keys_str_mv | AT fontangesquitterie clinicalapplicationoftargetednextgenerationsequencingforcolorectalcancers AT demendoncaricardo clinicalapplicationoftargetednextgenerationsequencingforcolorectalcancers AT salmonisabelle clinicalapplicationoftargetednextgenerationsequencingforcolorectalcancers AT lemerciermarie clinicalapplicationoftargetednextgenerationsequencingforcolorectalcancers AT dhaenenicky clinicalapplicationoftargetednextgenerationsequencingforcolorectalcancers |