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Tumour Heterogeneity: The Key Advantages of Single-Cell Analysis

Tumour heterogeneity refers to the fact that different tumour cells can show distinct morphological and phenotypic profiles, including cellular morphology, gene expression, metabolism, motility, proliferation and metastatic potential. This phenomenon occurs both between tumours (inter-tumour heterog...

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Autores principales: Tellez-Gabriel, Marta, Ory, Benjamin, Lamoureux, Francois, Heymann, Marie-Francoise, Heymann, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187942/
https://www.ncbi.nlm.nih.gov/pubmed/27999407
http://dx.doi.org/10.3390/ijms17122142
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author Tellez-Gabriel, Marta
Ory, Benjamin
Lamoureux, Francois
Heymann, Marie-Francoise
Heymann, Dominique
author_facet Tellez-Gabriel, Marta
Ory, Benjamin
Lamoureux, Francois
Heymann, Marie-Francoise
Heymann, Dominique
author_sort Tellez-Gabriel, Marta
collection PubMed
description Tumour heterogeneity refers to the fact that different tumour cells can show distinct morphological and phenotypic profiles, including cellular morphology, gene expression, metabolism, motility, proliferation and metastatic potential. This phenomenon occurs both between tumours (inter-tumour heterogeneity) and within tumours (intra-tumour heterogeneity), and it is caused by genetic and non-genetic factors. The heterogeneity of cancer cells introduces significant challenges in using molecular prognostic markers as well as for classifying patients that might benefit from specific therapies. Thus, research efforts for characterizing heterogeneity would be useful for a better understanding of the causes and progression of disease. It has been suggested that the study of heterogeneity within Circulating Tumour Cells (CTCs) could also reflect the full spectrum of mutations of the disease more accurately than a single biopsy of a primary or metastatic tumour. In previous years, many high throughput methodologies have raised for the study of heterogeneity at different levels (i.e., RNA, DNA, protein and epigenetic events). The aim of the current review is to stress clinical implications of tumour heterogeneity, as well as current available methodologies for their study, paying specific attention to those able to assess heterogeneity at the single cell level.
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spelling pubmed-51879422016-12-30 Tumour Heterogeneity: The Key Advantages of Single-Cell Analysis Tellez-Gabriel, Marta Ory, Benjamin Lamoureux, Francois Heymann, Marie-Francoise Heymann, Dominique Int J Mol Sci Review Tumour heterogeneity refers to the fact that different tumour cells can show distinct morphological and phenotypic profiles, including cellular morphology, gene expression, metabolism, motility, proliferation and metastatic potential. This phenomenon occurs both between tumours (inter-tumour heterogeneity) and within tumours (intra-tumour heterogeneity), and it is caused by genetic and non-genetic factors. The heterogeneity of cancer cells introduces significant challenges in using molecular prognostic markers as well as for classifying patients that might benefit from specific therapies. Thus, research efforts for characterizing heterogeneity would be useful for a better understanding of the causes and progression of disease. It has been suggested that the study of heterogeneity within Circulating Tumour Cells (CTCs) could also reflect the full spectrum of mutations of the disease more accurately than a single biopsy of a primary or metastatic tumour. In previous years, many high throughput methodologies have raised for the study of heterogeneity at different levels (i.e., RNA, DNA, protein and epigenetic events). The aim of the current review is to stress clinical implications of tumour heterogeneity, as well as current available methodologies for their study, paying specific attention to those able to assess heterogeneity at the single cell level. MDPI 2016-12-20 /pmc/articles/PMC5187942/ /pubmed/27999407 http://dx.doi.org/10.3390/ijms17122142 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Tellez-Gabriel, Marta
Ory, Benjamin
Lamoureux, Francois
Heymann, Marie-Francoise
Heymann, Dominique
Tumour Heterogeneity: The Key Advantages of Single-Cell Analysis
title Tumour Heterogeneity: The Key Advantages of Single-Cell Analysis
title_full Tumour Heterogeneity: The Key Advantages of Single-Cell Analysis
title_fullStr Tumour Heterogeneity: The Key Advantages of Single-Cell Analysis
title_full_unstemmed Tumour Heterogeneity: The Key Advantages of Single-Cell Analysis
title_short Tumour Heterogeneity: The Key Advantages of Single-Cell Analysis
title_sort tumour heterogeneity: the key advantages of single-cell analysis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187942/
https://www.ncbi.nlm.nih.gov/pubmed/27999407
http://dx.doi.org/10.3390/ijms17122142
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