Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France

Using samples from oysters clearly implicated in human disease, we quantified norovirus levels by using digital PCR. Concentrations varied from 43 to 1,170 RNA copies/oyster. The analysis of frozen samples from the production area showed the presence of norovirus 2 weeks before consumption.

Detalles Bibliográficos
Autores principales: Polo, David, Schaeffer, Julien, Fournet, Nelly, Le Saux, Jean-Claude, Parnaudeau, Sylvain, McLeod, Catherine, Le Guyader, Françoise S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2016
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5189157/
https://www.ncbi.nlm.nih.gov/pubmed/27869597
http://dx.doi.org/10.3201/eid2212.160841
Descripción
Sumario:Using samples from oysters clearly implicated in human disease, we quantified norovirus levels by using digital PCR. Concentrations varied from 43 to 1,170 RNA copies/oyster. The analysis of frozen samples from the production area showed the presence of norovirus 2 weeks before consumption.

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