Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France

Using samples from oysters clearly implicated in human disease, we quantified norovirus levels by using digital PCR. Concentrations varied from 43 to 1,170 RNA copies/oyster. The analysis of frozen samples from the production area showed the presence of norovirus 2 weeks before consumption.

Detalles Bibliográficos
Autores principales: Polo, David, Schaeffer, Julien, Fournet, Nelly, Le Saux, Jean-Claude, Parnaudeau, Sylvain, McLeod, Catherine, Le Guyader, Françoise S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2016
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5189157/
https://www.ncbi.nlm.nih.gov/pubmed/27869597
http://dx.doi.org/10.3201/eid2212.160841
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author Polo, David
Schaeffer, Julien
Fournet, Nelly
Le Saux, Jean-Claude
Parnaudeau, Sylvain
McLeod, Catherine
Le Guyader, Françoise S.
author_facet Polo, David
Schaeffer, Julien
Fournet, Nelly
Le Saux, Jean-Claude
Parnaudeau, Sylvain
McLeod, Catherine
Le Guyader, Françoise S.
author_sort Polo, David
collection PubMed
description Using samples from oysters clearly implicated in human disease, we quantified norovirus levels by using digital PCR. Concentrations varied from 43 to 1,170 RNA copies/oyster. The analysis of frozen samples from the production area showed the presence of norovirus 2 weeks before consumption.
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spelling pubmed-51891572016-12-29 Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France Polo, David Schaeffer, Julien Fournet, Nelly Le Saux, Jean-Claude Parnaudeau, Sylvain McLeod, Catherine Le Guyader, Françoise S. Emerg Infect Dis Dispatch Using samples from oysters clearly implicated in human disease, we quantified norovirus levels by using digital PCR. Concentrations varied from 43 to 1,170 RNA copies/oyster. The analysis of frozen samples from the production area showed the presence of norovirus 2 weeks before consumption. Centers for Disease Control and Prevention 2016-12 /pmc/articles/PMC5189157/ /pubmed/27869597 http://dx.doi.org/10.3201/eid2212.160841 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Polo, David
Schaeffer, Julien
Fournet, Nelly
Le Saux, Jean-Claude
Parnaudeau, Sylvain
McLeod, Catherine
Le Guyader, Françoise S.
Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France
title Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France
title_full Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France
title_fullStr Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France
title_full_unstemmed Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France
title_short Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France
title_sort digital pcr for quantifying norovirus in oysters implicated in outbreaks, france
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5189157/
https://www.ncbi.nlm.nih.gov/pubmed/27869597
http://dx.doi.org/10.3201/eid2212.160841
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