Cargando…

High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma

PURPOSE: Emerging studies show that long noncoding RNAs (lncRNAs) have important roles in carcinogenesis. This study investigated the role of lncRNA highly upregulated in liver cancer (HULC) expression in glioma and its clinical significance in glioma patients. MATERIALS AND METHODS: HULC expression...

Descripción completa

Detalles Bibliográficos
Autores principales: Yan, Hong, Tian, Rui, Zhang, Min, Wu, Jing, Ding, Min, He, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5189971/
https://www.ncbi.nlm.nih.gov/pubmed/28053545
http://dx.doi.org/10.2147/OTT.S124614
_version_ 1782487325357375488
author Yan, Hong
Tian, Rui
Zhang, Min
Wu, Jing
Ding, Min
He, Jie
author_facet Yan, Hong
Tian, Rui
Zhang, Min
Wu, Jing
Ding, Min
He, Jie
author_sort Yan, Hong
collection PubMed
description PURPOSE: Emerging studies show that long noncoding RNAs (lncRNAs) have important roles in carcinogenesis. This study investigated the role of lncRNA highly upregulated in liver cancer (HULC) expression in glioma and its clinical significance in glioma patients. MATERIALS AND METHODS: HULC expression was detected in glioma tissues and cell lines by using real-time quantitative reverse transcription polymerase chain reactions. Association between HULC levels and clinicopathological factors and patients prognosis was also analyzed. Expression of HULC was restored and knocked down in glioma cell line U87 by using HULC cDNA and siRNA, respectively. CCK-8 and colony formation assays were used to investigate the role of HULC in the regulation of proliferation of glioma cells. RESULTS: HULC was highly expressed in glioma tissues, being closely related to age and grade of glioma. Univariate survival analysis demonstrated that high HULC levels were significantly associated with overall survival (OS) (hazard ratio [HR], 0.422; 95% confidence interval [CI], 0.220–0.806; P=0.009), and it remained an independent predictor for OS (HR, 0.340; 95% CI, 0.175–0.659; P=0.001) in multivariate Cox regression analysis. Functionally, forced expression of HULC results in increased cell proliferation and colony formation of U87 glioma cell line, whereas knockdown of HULC expression reduced these oncogenic properties of glioma cells. CONCLUSION: These findings suggest that HULC may play an important role in glioma progression and will be further evaluated as a biomarker for predicting the survival of glioma patients.
format Online
Article
Text
id pubmed-5189971
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-51899712017-01-04 High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma Yan, Hong Tian, Rui Zhang, Min Wu, Jing Ding, Min He, Jie Onco Targets Ther Original Research PURPOSE: Emerging studies show that long noncoding RNAs (lncRNAs) have important roles in carcinogenesis. This study investigated the role of lncRNA highly upregulated in liver cancer (HULC) expression in glioma and its clinical significance in glioma patients. MATERIALS AND METHODS: HULC expression was detected in glioma tissues and cell lines by using real-time quantitative reverse transcription polymerase chain reactions. Association between HULC levels and clinicopathological factors and patients prognosis was also analyzed. Expression of HULC was restored and knocked down in glioma cell line U87 by using HULC cDNA and siRNA, respectively. CCK-8 and colony formation assays were used to investigate the role of HULC in the regulation of proliferation of glioma cells. RESULTS: HULC was highly expressed in glioma tissues, being closely related to age and grade of glioma. Univariate survival analysis demonstrated that high HULC levels were significantly associated with overall survival (OS) (hazard ratio [HR], 0.422; 95% confidence interval [CI], 0.220–0.806; P=0.009), and it remained an independent predictor for OS (HR, 0.340; 95% CI, 0.175–0.659; P=0.001) in multivariate Cox regression analysis. Functionally, forced expression of HULC results in increased cell proliferation and colony formation of U87 glioma cell line, whereas knockdown of HULC expression reduced these oncogenic properties of glioma cells. CONCLUSION: These findings suggest that HULC may play an important role in glioma progression and will be further evaluated as a biomarker for predicting the survival of glioma patients. Dove Medical Press 2016-12-21 /pmc/articles/PMC5189971/ /pubmed/28053545 http://dx.doi.org/10.2147/OTT.S124614 Text en © 2017 Yan et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Yan, Hong
Tian, Rui
Zhang, Min
Wu, Jing
Ding, Min
He, Jie
High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma
title High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma
title_full High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma
title_fullStr High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma
title_full_unstemmed High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma
title_short High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma
title_sort high expression of long noncoding rna hulc is a poor predictor of prognosis and regulates cell proliferation in glioma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5189971/
https://www.ncbi.nlm.nih.gov/pubmed/28053545
http://dx.doi.org/10.2147/OTT.S124614
work_keys_str_mv AT yanhong highexpressionoflongnoncodingrnahulcisapoorpredictorofprognosisandregulatescellproliferationinglioma
AT tianrui highexpressionoflongnoncodingrnahulcisapoorpredictorofprognosisandregulatescellproliferationinglioma
AT zhangmin highexpressionoflongnoncodingrnahulcisapoorpredictorofprognosisandregulatescellproliferationinglioma
AT wujing highexpressionoflongnoncodingrnahulcisapoorpredictorofprognosisandregulatescellproliferationinglioma
AT dingmin highexpressionoflongnoncodingrnahulcisapoorpredictorofprognosisandregulatescellproliferationinglioma
AT hejie highexpressionoflongnoncodingrnahulcisapoorpredictorofprognosisandregulatescellproliferationinglioma