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MicroRNA-187 inhibits tumor growth and invasion by directly targeting CD276 in colorectal cancer

Aberrantly expressed microRNAs contribute to the initiation and progression of human cancers. However, the underlying functions of microRNA-187 (miR-187) in colorectal cancer (CRC) remain largely unexplored. Here, we demonstrated that miR-187 was significantly down-regulated in CRC tissues and cell...

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Autores principales: Wang, Zheng-Shi, Zhong, Ming, Bian, Yu-Hai, Mu, Yi-Fei, Qin, Shao-Lan, Yu, Min-Hao, Qin, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5190094/
https://www.ncbi.nlm.nih.gov/pubmed/27329595
http://dx.doi.org/10.18632/oncotarget.10023
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author Wang, Zheng-Shi
Zhong, Ming
Bian, Yu-Hai
Mu, Yi-Fei
Qin, Shao-Lan
Yu, Min-Hao
Qin, Jun
author_facet Wang, Zheng-Shi
Zhong, Ming
Bian, Yu-Hai
Mu, Yi-Fei
Qin, Shao-Lan
Yu, Min-Hao
Qin, Jun
author_sort Wang, Zheng-Shi
collection PubMed
description Aberrantly expressed microRNAs contribute to the initiation and progression of human cancers. However, the underlying functions of microRNA-187 (miR-187) in colorectal cancer (CRC) remain largely unexplored. Here, we demonstrated that miR-187 was significantly down-regulated in CRC tissues and cell lines compared to their normal counterparts. By Kaplan-Meier analysis, we revealed that decreased miR-187 expression was closely associated with shorter overall survival and relapse-free survival of patients with CRC. By gain- and loss-of-function studies, we showed that miR-187 remarkably suppressed CRC cell proliferation, migration, invasion, and promoted cell apoptosis. Furthermore, bioinformatics analysis and luciferase reporter assay identified that CD276 was the direct functional target of miR-187 in CRC. Genetic silencing of CD276 recapitulated similar phenotype as observed in over-expression of miR-187, and restoration of CD276 completely rescued the inhibitory effect of miR-187 in CRC cells. Taken together, our study implied the essential roles of miR-187 in suppressing CRC progression, and a novel link between miR-187 and CD276 in CRC.
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spelling pubmed-51900942017-01-05 MicroRNA-187 inhibits tumor growth and invasion by directly targeting CD276 in colorectal cancer Wang, Zheng-Shi Zhong, Ming Bian, Yu-Hai Mu, Yi-Fei Qin, Shao-Lan Yu, Min-Hao Qin, Jun Oncotarget Research Paper Aberrantly expressed microRNAs contribute to the initiation and progression of human cancers. However, the underlying functions of microRNA-187 (miR-187) in colorectal cancer (CRC) remain largely unexplored. Here, we demonstrated that miR-187 was significantly down-regulated in CRC tissues and cell lines compared to their normal counterparts. By Kaplan-Meier analysis, we revealed that decreased miR-187 expression was closely associated with shorter overall survival and relapse-free survival of patients with CRC. By gain- and loss-of-function studies, we showed that miR-187 remarkably suppressed CRC cell proliferation, migration, invasion, and promoted cell apoptosis. Furthermore, bioinformatics analysis and luciferase reporter assay identified that CD276 was the direct functional target of miR-187 in CRC. Genetic silencing of CD276 recapitulated similar phenotype as observed in over-expression of miR-187, and restoration of CD276 completely rescued the inhibitory effect of miR-187 in CRC cells. Taken together, our study implied the essential roles of miR-187 in suppressing CRC progression, and a novel link between miR-187 and CD276 in CRC. Impact Journals LLC 2016-06-14 /pmc/articles/PMC5190094/ /pubmed/27329595 http://dx.doi.org/10.18632/oncotarget.10023 Text en Copyright: © 2016 Wang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wang, Zheng-Shi
Zhong, Ming
Bian, Yu-Hai
Mu, Yi-Fei
Qin, Shao-Lan
Yu, Min-Hao
Qin, Jun
MicroRNA-187 inhibits tumor growth and invasion by directly targeting CD276 in colorectal cancer
title MicroRNA-187 inhibits tumor growth and invasion by directly targeting CD276 in colorectal cancer
title_full MicroRNA-187 inhibits tumor growth and invasion by directly targeting CD276 in colorectal cancer
title_fullStr MicroRNA-187 inhibits tumor growth and invasion by directly targeting CD276 in colorectal cancer
title_full_unstemmed MicroRNA-187 inhibits tumor growth and invasion by directly targeting CD276 in colorectal cancer
title_short MicroRNA-187 inhibits tumor growth and invasion by directly targeting CD276 in colorectal cancer
title_sort microrna-187 inhibits tumor growth and invasion by directly targeting cd276 in colorectal cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5190094/
https://www.ncbi.nlm.nih.gov/pubmed/27329595
http://dx.doi.org/10.18632/oncotarget.10023
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