Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor

Herein, we report on the fabrication of an extended-gated organic field-effect transistor (OFET)-based immunosensor and its application in the detection of human chromogranin A (hCgA). The fabricated OFET device possesses an extended-gate electrode immobilized with an anti-CgA antibody. The titratio...

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Autores principales: Minamiki, Tsukuru, Minami, Tsuyoshi, Sasaki, Yui, Wakida, Shin-ichi, Kurita, Ryoji, Niwa, Osamu, Tokito, Shizuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191014/
https://www.ncbi.nlm.nih.gov/pubmed/27916899
http://dx.doi.org/10.3390/s16122033
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author Minamiki, Tsukuru
Minami, Tsuyoshi
Sasaki, Yui
Wakida, Shin-ichi
Kurita, Ryoji
Niwa, Osamu
Tokito, Shizuo
author_facet Minamiki, Tsukuru
Minami, Tsuyoshi
Sasaki, Yui
Wakida, Shin-ichi
Kurita, Ryoji
Niwa, Osamu
Tokito, Shizuo
author_sort Minamiki, Tsukuru
collection PubMed
description Herein, we report on the fabrication of an extended-gated organic field-effect transistor (OFET)-based immunosensor and its application in the detection of human chromogranin A (hCgA). The fabricated OFET device possesses an extended-gate electrode immobilized with an anti-CgA antibody. The titration results of hCgA showed that the electrical changes in the OFET characteristics corresponded to the glycoprotein recognition ability of the monoclonal antibody (anti-CgA). The observed sensitivity (detection limit: 0.11 µg/mL) and selectivity indicate that the OFET-based immunosensor can be potentially applied to the rapid detection of the glycoprotein concentration without any labeling.
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spelling pubmed-51910142017-01-03 Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor Minamiki, Tsukuru Minami, Tsuyoshi Sasaki, Yui Wakida, Shin-ichi Kurita, Ryoji Niwa, Osamu Tokito, Shizuo Sensors (Basel) Communication Herein, we report on the fabrication of an extended-gated organic field-effect transistor (OFET)-based immunosensor and its application in the detection of human chromogranin A (hCgA). The fabricated OFET device possesses an extended-gate electrode immobilized with an anti-CgA antibody. The titration results of hCgA showed that the electrical changes in the OFET characteristics corresponded to the glycoprotein recognition ability of the monoclonal antibody (anti-CgA). The observed sensitivity (detection limit: 0.11 µg/mL) and selectivity indicate that the OFET-based immunosensor can be potentially applied to the rapid detection of the glycoprotein concentration without any labeling. MDPI 2016-11-30 /pmc/articles/PMC5191014/ /pubmed/27916899 http://dx.doi.org/10.3390/s16122033 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Minamiki, Tsukuru
Minami, Tsuyoshi
Sasaki, Yui
Wakida, Shin-ichi
Kurita, Ryoji
Niwa, Osamu
Tokito, Shizuo
Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor
title Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor
title_full Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor
title_fullStr Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor
title_full_unstemmed Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor
title_short Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor
title_sort label-free detection of human glycoprotein (cga) using an extended-gated organic transistor-based immunosensor
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191014/
https://www.ncbi.nlm.nih.gov/pubmed/27916899
http://dx.doi.org/10.3390/s16122033
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