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Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells

Manipulation and injection of single nanosensors with high cell viability is an emerging field in cell analysis. We propose a new method using fluorescence nanosensors with a glass nanoprobe and optical control of the zeta potential. The nanosensor is fabricated by encapsulating a fluorescence polys...

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Autores principales: Hashim, Hairulazwan, Maruyama, Hisataka, Masuda, Taisuke, Arai, Fumihito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191022/
https://www.ncbi.nlm.nih.gov/pubmed/27916931
http://dx.doi.org/10.3390/s16122041
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author Hashim, Hairulazwan
Maruyama, Hisataka
Masuda, Taisuke
Arai, Fumihito
author_facet Hashim, Hairulazwan
Maruyama, Hisataka
Masuda, Taisuke
Arai, Fumihito
author_sort Hashim, Hairulazwan
collection PubMed
description Manipulation and injection of single nanosensors with high cell viability is an emerging field in cell analysis. We propose a new method using fluorescence nanosensors with a glass nanoprobe and optical control of the zeta potential. The nanosensor is fabricated by encapsulating a fluorescence polystyrene nanobead into a lipid layer with 1,3,3-trimethylindolino-6′-nitrobenzopyrylospiran (SP), which is a photochromic material. The nanobead contains iron oxide nanoparticles and a temperature-sensitive fluorescent dye, Rhodamine B. The zeta potential of the nanosensor switches between negative and positive by photo-isomerization of SP with ultraviolet irradiation. The positively-charged nanosensor easily adheres to a negatively-charged glass nanoprobe, is transported to a target cell, and then adheres to the negatively-charged cell membrane. The nanosensor is then injected into the cytoplasm by heating with a near-infrared (NIR) laser. As a demonstration, a single 750 nm nanosensor was picked-up using a glass nanoprobe with optical control of the zeta potential. Then, the nanosensor was transported and immobilized onto a target cell membrane. Finally, it was injected into the cytoplasm using a NIR laser. The success rates of pick-up and cell immobilization of the nanosensor were 75% and 64%, respectively. Cell injection and cell survival rates were 80% and 100%, respectively.
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spelling pubmed-51910222017-01-03 Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells Hashim, Hairulazwan Maruyama, Hisataka Masuda, Taisuke Arai, Fumihito Sensors (Basel) Article Manipulation and injection of single nanosensors with high cell viability is an emerging field in cell analysis. We propose a new method using fluorescence nanosensors with a glass nanoprobe and optical control of the zeta potential. The nanosensor is fabricated by encapsulating a fluorescence polystyrene nanobead into a lipid layer with 1,3,3-trimethylindolino-6′-nitrobenzopyrylospiran (SP), which is a photochromic material. The nanobead contains iron oxide nanoparticles and a temperature-sensitive fluorescent dye, Rhodamine B. The zeta potential of the nanosensor switches between negative and positive by photo-isomerization of SP with ultraviolet irradiation. The positively-charged nanosensor easily adheres to a negatively-charged glass nanoprobe, is transported to a target cell, and then adheres to the negatively-charged cell membrane. The nanosensor is then injected into the cytoplasm by heating with a near-infrared (NIR) laser. As a demonstration, a single 750 nm nanosensor was picked-up using a glass nanoprobe with optical control of the zeta potential. Then, the nanosensor was transported and immobilized onto a target cell membrane. Finally, it was injected into the cytoplasm using a NIR laser. The success rates of pick-up and cell immobilization of the nanosensor were 75% and 64%, respectively. Cell injection and cell survival rates were 80% and 100%, respectively. MDPI 2016-12-01 /pmc/articles/PMC5191022/ /pubmed/27916931 http://dx.doi.org/10.3390/s16122041 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hashim, Hairulazwan
Maruyama, Hisataka
Masuda, Taisuke
Arai, Fumihito
Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells
title Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells
title_full Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells
title_fullStr Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells
title_full_unstemmed Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells
title_short Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells
title_sort manipulation and immobilization of a single fluorescence nanosensor for selective injection into cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191022/
https://www.ncbi.nlm.nih.gov/pubmed/27916931
http://dx.doi.org/10.3390/s16122041
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