Cargando…
Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs
Group I self-splicing intron constitutes an important class of functional RNA molecules that can promote chemical transformation. Although the fundamental mechanism of the auto-excision from its precursor RNA has been established, convenient assay systems for its splicing activity are still useful f...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192423/ https://www.ncbi.nlm.nih.gov/pubmed/27869660 http://dx.doi.org/10.3390/biology5040043 |
_version_ | 1782487773195796480 |
---|---|
author | Furukawa, Airi Tanaka, Takahiro Furuta, Hiroyuki Matsumura, Shigeyoshi Ikawa, Yoshiya |
author_facet | Furukawa, Airi Tanaka, Takahiro Furuta, Hiroyuki Matsumura, Shigeyoshi Ikawa, Yoshiya |
author_sort | Furukawa, Airi |
collection | PubMed |
description | Group I self-splicing intron constitutes an important class of functional RNA molecules that can promote chemical transformation. Although the fundamental mechanism of the auto-excision from its precursor RNA has been established, convenient assay systems for its splicing activity are still useful for a further understanding of its detailed mechanism and of its application. Because some host RNA sequences, to which group I introns inserted form stable three-dimensional (3D) structures, the effects of the 3D structures of exonic elements on the splicing efficiency of group I introns are important but not a fully investigated issue. We developed an assay system for group I intron self-splicing by employing a fluorescent aptamer RNA (spinach RNA) as a model exonic sequence inserted by the Tetrahymena group I intron. We investigated self-splicing of the intron from spinach RNA, serving as a model exonic sequence with a 3D structure. |
format | Online Article Text |
id | pubmed-5192423 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-51924232017-01-03 Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs Furukawa, Airi Tanaka, Takahiro Furuta, Hiroyuki Matsumura, Shigeyoshi Ikawa, Yoshiya Biology (Basel) Article Group I self-splicing intron constitutes an important class of functional RNA molecules that can promote chemical transformation. Although the fundamental mechanism of the auto-excision from its precursor RNA has been established, convenient assay systems for its splicing activity are still useful for a further understanding of its detailed mechanism and of its application. Because some host RNA sequences, to which group I introns inserted form stable three-dimensional (3D) structures, the effects of the 3D structures of exonic elements on the splicing efficiency of group I introns are important but not a fully investigated issue. We developed an assay system for group I intron self-splicing by employing a fluorescent aptamer RNA (spinach RNA) as a model exonic sequence inserted by the Tetrahymena group I intron. We investigated self-splicing of the intron from spinach RNA, serving as a model exonic sequence with a 3D structure. MDPI 2016-11-17 /pmc/articles/PMC5192423/ /pubmed/27869660 http://dx.doi.org/10.3390/biology5040043 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Furukawa, Airi Tanaka, Takahiro Furuta, Hiroyuki Matsumura, Shigeyoshi Ikawa, Yoshiya Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
title | Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
title_full | Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
title_fullStr | Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
title_full_unstemmed | Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
title_short | Use of a Fluorescent Aptamer RNA as an Exonic Sequence to Analyze Self-Splicing Ability of a Group I Intron from Structured RNAs |
title_sort | use of a fluorescent aptamer rna as an exonic sequence to analyze self-splicing ability of a group i intron from structured rnas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192423/ https://www.ncbi.nlm.nih.gov/pubmed/27869660 http://dx.doi.org/10.3390/biology5040043 |
work_keys_str_mv | AT furukawaairi useofafluorescentaptamerrnaasanexonicsequencetoanalyzeselfsplicingabilityofagroupiintronfromstructuredrnas AT tanakatakahiro useofafluorescentaptamerrnaasanexonicsequencetoanalyzeselfsplicingabilityofagroupiintronfromstructuredrnas AT furutahiroyuki useofafluorescentaptamerrnaasanexonicsequencetoanalyzeselfsplicingabilityofagroupiintronfromstructuredrnas AT matsumurashigeyoshi useofafluorescentaptamerrnaasanexonicsequencetoanalyzeselfsplicingabilityofagroupiintronfromstructuredrnas AT ikawayoshiya useofafluorescentaptamerrnaasanexonicsequencetoanalyzeselfsplicingabilityofagroupiintronfromstructuredrnas |