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Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis

Patients surviving sepsis demonstrate sustained inflammation, which has been associated with long-term complications. One of the main mechanisms behind sustained inflammation is a metabolic switch in parenchymal and immune cells, thus understanding metabolic alterations after sepsis may provide impo...

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Autores principales: Bakalov, Veli, Amathieu, Roland, Triba, Mohamed N., Clément, Marie-Jeanne, Reyes Uribe, Laura, Le Moyec, Laurence, Kaynar, Ata Murat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192453/
https://www.ncbi.nlm.nih.gov/pubmed/28009836
http://dx.doi.org/10.3390/metabo6040047
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author Bakalov, Veli
Amathieu, Roland
Triba, Mohamed N.
Clément, Marie-Jeanne
Reyes Uribe, Laura
Le Moyec, Laurence
Kaynar, Ata Murat
author_facet Bakalov, Veli
Amathieu, Roland
Triba, Mohamed N.
Clément, Marie-Jeanne
Reyes Uribe, Laura
Le Moyec, Laurence
Kaynar, Ata Murat
author_sort Bakalov, Veli
collection PubMed
description Patients surviving sepsis demonstrate sustained inflammation, which has been associated with long-term complications. One of the main mechanisms behind sustained inflammation is a metabolic switch in parenchymal and immune cells, thus understanding metabolic alterations after sepsis may provide important insights to the pathophysiology of sepsis recovery. In this study, we explored metabolomics in a novel Drosophila melanogaster model of surviving sepsis using Nuclear Magnetic Resonance (NMR), to determine metabolite profiles. We used a model of percutaneous infection in Drosophila melanogaster to mimic sepsis. We had three experimental groups: sepsis survivors (infected with Staphylococcus aureus and treated with oral linezolid), sham (pricked with an aseptic needle), and unmanipulated (positive control). We performed metabolic measurements seven days after sepsis. We then implemented metabolites detected in NMR spectra into the MetExplore web server in order to identify the metabolic pathway alterations in sepsis surviving Drosophila. Our NMR metabolomic approach in a Drosophila model of recovery from sepsis clearly distinguished between all three groups and showed two different metabolomic signatures of inflammation. Sham flies had decreased levels of maltose, alanine, and glutamine, while their level of choline was increased. Sepsis survivors had a metabolic signature characterized by decreased glucose, maltose, tyrosine, beta-alanine, acetate, glutamine, and succinate.
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spelling pubmed-51924532017-01-03 Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis Bakalov, Veli Amathieu, Roland Triba, Mohamed N. Clément, Marie-Jeanne Reyes Uribe, Laura Le Moyec, Laurence Kaynar, Ata Murat Metabolites Article Patients surviving sepsis demonstrate sustained inflammation, which has been associated with long-term complications. One of the main mechanisms behind sustained inflammation is a metabolic switch in parenchymal and immune cells, thus understanding metabolic alterations after sepsis may provide important insights to the pathophysiology of sepsis recovery. In this study, we explored metabolomics in a novel Drosophila melanogaster model of surviving sepsis using Nuclear Magnetic Resonance (NMR), to determine metabolite profiles. We used a model of percutaneous infection in Drosophila melanogaster to mimic sepsis. We had three experimental groups: sepsis survivors (infected with Staphylococcus aureus and treated with oral linezolid), sham (pricked with an aseptic needle), and unmanipulated (positive control). We performed metabolic measurements seven days after sepsis. We then implemented metabolites detected in NMR spectra into the MetExplore web server in order to identify the metabolic pathway alterations in sepsis surviving Drosophila. Our NMR metabolomic approach in a Drosophila model of recovery from sepsis clearly distinguished between all three groups and showed two different metabolomic signatures of inflammation. Sham flies had decreased levels of maltose, alanine, and glutamine, while their level of choline was increased. Sepsis survivors had a metabolic signature characterized by decreased glucose, maltose, tyrosine, beta-alanine, acetate, glutamine, and succinate. MDPI 2016-12-21 /pmc/articles/PMC5192453/ /pubmed/28009836 http://dx.doi.org/10.3390/metabo6040047 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bakalov, Veli
Amathieu, Roland
Triba, Mohamed N.
Clément, Marie-Jeanne
Reyes Uribe, Laura
Le Moyec, Laurence
Kaynar, Ata Murat
Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis
title Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis
title_full Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis
title_fullStr Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis
title_full_unstemmed Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis
title_short Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis
title_sort metabolomics with nuclear magnetic resonance spectroscopy in a drosophila melanogaster model of surviving sepsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192453/
https://www.ncbi.nlm.nih.gov/pubmed/28009836
http://dx.doi.org/10.3390/metabo6040047
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