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Protective Effects of Hydrolyzed Nucleoproteins from Salmon Milt against Ethanol-Induced Liver Injury in Rats

Dietary nucleotides play a role in maintaining the immune responses of both animals and humans. Oral administration of nucleic acids from salmon milt have physiological functions in the cellular metabolism, proliferation, differentiation, and apoptosis of human small intestinal epithelial cells. In...

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Autores principales: Kojima-Yuasa, Akiko, Goto, Mayu, Yoshikawa, Eri, Morita, Yuri, Sekiguchi, Hirotaka, Sutoh, Keita, Usumi, Koji, Matsui-Yuasa, Isao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192469/
https://www.ncbi.nlm.nih.gov/pubmed/27999369
http://dx.doi.org/10.3390/md14120232
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author Kojima-Yuasa, Akiko
Goto, Mayu
Yoshikawa, Eri
Morita, Yuri
Sekiguchi, Hirotaka
Sutoh, Keita
Usumi, Koji
Matsui-Yuasa, Isao
author_facet Kojima-Yuasa, Akiko
Goto, Mayu
Yoshikawa, Eri
Morita, Yuri
Sekiguchi, Hirotaka
Sutoh, Keita
Usumi, Koji
Matsui-Yuasa, Isao
author_sort Kojima-Yuasa, Akiko
collection PubMed
description Dietary nucleotides play a role in maintaining the immune responses of both animals and humans. Oral administration of nucleic acids from salmon milt have physiological functions in the cellular metabolism, proliferation, differentiation, and apoptosis of human small intestinal epithelial cells. In this study, we examined the effects of DNA-rich nucleic acids prepared from salmon milt (DNSM) on the development of liver fibrosis in an in vivo ethanol-carbon tetrachloride cirrhosis model. Plasma aspartate transaminase and alanine transaminase were significantly less active in the DNSM-treated group than in the ethanol plus carbon tetrachloride (CCl(4))-treated group. Collagen accumulation in the liver and hepatic necrosis were observed histologically in ethanol plus CCl(4)-treated rats; however, DNSM-treatment fully protected rats against ethanol plus CCl(4)-induced liver fibrosis and necrosis. Furthermore, we examined whether DNSM had a preventive effect against alcohol-induced liver injury by regulating the cytochrome p450 2E1 (CYP2E1)-mediated oxidative stress pathway in an in vivo model. In this model, CYP2E1 activity in ethanol plus CCl(4)-treated rats increased significantly, but DNSM-treatment suppressed the enzyme’s activity and reduced intracellular thiobarbituric acid reactive substances (TBARS) levels. Furthermore, the hepatocytes treated with 100 mM ethanol induced an increase in cell death and were not restored to the control levels when treated with DNSM, suggesting that digestive products of DNSM are effective for the prevention of alcohol-induced liver injury. Deoxyadenosine suppressed the ethanol-induced increase in cell death and increased the activity of alcohol dehydrogenase. These results suggest that DNSM treatment represents a novel tool for the prevention of alcohol-induced liver injury.
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spelling pubmed-51924692017-01-03 Protective Effects of Hydrolyzed Nucleoproteins from Salmon Milt against Ethanol-Induced Liver Injury in Rats Kojima-Yuasa, Akiko Goto, Mayu Yoshikawa, Eri Morita, Yuri Sekiguchi, Hirotaka Sutoh, Keita Usumi, Koji Matsui-Yuasa, Isao Mar Drugs Article Dietary nucleotides play a role in maintaining the immune responses of both animals and humans. Oral administration of nucleic acids from salmon milt have physiological functions in the cellular metabolism, proliferation, differentiation, and apoptosis of human small intestinal epithelial cells. In this study, we examined the effects of DNA-rich nucleic acids prepared from salmon milt (DNSM) on the development of liver fibrosis in an in vivo ethanol-carbon tetrachloride cirrhosis model. Plasma aspartate transaminase and alanine transaminase were significantly less active in the DNSM-treated group than in the ethanol plus carbon tetrachloride (CCl(4))-treated group. Collagen accumulation in the liver and hepatic necrosis were observed histologically in ethanol plus CCl(4)-treated rats; however, DNSM-treatment fully protected rats against ethanol plus CCl(4)-induced liver fibrosis and necrosis. Furthermore, we examined whether DNSM had a preventive effect against alcohol-induced liver injury by regulating the cytochrome p450 2E1 (CYP2E1)-mediated oxidative stress pathway in an in vivo model. In this model, CYP2E1 activity in ethanol plus CCl(4)-treated rats increased significantly, but DNSM-treatment suppressed the enzyme’s activity and reduced intracellular thiobarbituric acid reactive substances (TBARS) levels. Furthermore, the hepatocytes treated with 100 mM ethanol induced an increase in cell death and were not restored to the control levels when treated with DNSM, suggesting that digestive products of DNSM are effective for the prevention of alcohol-induced liver injury. Deoxyadenosine suppressed the ethanol-induced increase in cell death and increased the activity of alcohol dehydrogenase. These results suggest that DNSM treatment represents a novel tool for the prevention of alcohol-induced liver injury. MDPI 2016-12-19 /pmc/articles/PMC5192469/ /pubmed/27999369 http://dx.doi.org/10.3390/md14120232 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kojima-Yuasa, Akiko
Goto, Mayu
Yoshikawa, Eri
Morita, Yuri
Sekiguchi, Hirotaka
Sutoh, Keita
Usumi, Koji
Matsui-Yuasa, Isao
Protective Effects of Hydrolyzed Nucleoproteins from Salmon Milt against Ethanol-Induced Liver Injury in Rats
title Protective Effects of Hydrolyzed Nucleoproteins from Salmon Milt against Ethanol-Induced Liver Injury in Rats
title_full Protective Effects of Hydrolyzed Nucleoproteins from Salmon Milt against Ethanol-Induced Liver Injury in Rats
title_fullStr Protective Effects of Hydrolyzed Nucleoproteins from Salmon Milt against Ethanol-Induced Liver Injury in Rats
title_full_unstemmed Protective Effects of Hydrolyzed Nucleoproteins from Salmon Milt against Ethanol-Induced Liver Injury in Rats
title_short Protective Effects of Hydrolyzed Nucleoproteins from Salmon Milt against Ethanol-Induced Liver Injury in Rats
title_sort protective effects of hydrolyzed nucleoproteins from salmon milt against ethanol-induced liver injury in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192469/
https://www.ncbi.nlm.nih.gov/pubmed/27999369
http://dx.doi.org/10.3390/md14120232
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