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In Silico Characterization of miRNA and Long Non-Coding RNA Interplay in Multiple Myeloma
The identification of deregulated microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in multiple myeloma (MM) has progressively added a further level of complexity to MM biology. In addition, the cross-regulation between lncRNAs and miRNAs has begun to emerge, and theoretical and experimental stu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192483/ https://www.ncbi.nlm.nih.gov/pubmed/27916857 http://dx.doi.org/10.3390/genes7120107 |
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author | Ronchetti, Domenica Manzoni, Martina Todoerti, Katia Neri, Antonino Agnelli, Luca |
author_facet | Ronchetti, Domenica Manzoni, Martina Todoerti, Katia Neri, Antonino Agnelli, Luca |
author_sort | Ronchetti, Domenica |
collection | PubMed |
description | The identification of deregulated microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in multiple myeloma (MM) has progressively added a further level of complexity to MM biology. In addition, the cross-regulation between lncRNAs and miRNAs has begun to emerge, and theoretical and experimental studies have demonstrated the competing endogenous RNA (ceRNA) activity of lncRNAs as natural miRNA decoys in pathophysiological conditions, including cancer. Currently, information concerning lncRNA and miRNA interplay in MM is virtually absent. Herein, we investigated in silico the lncRNA and miRNA relationship in a representative datasets encompassing 95 MM and 30 plasma cell leukemia patients at diagnosis and in four normal controls, whose expression profiles were generated by a custom annotation pipeline to detect specific lncRNAs. We applied target prediction analysis based on miRanda and RNA22 algorithms to 235 lncRNAs and 459 miRNAs selected with a potential pivotal role in the pathology of MM. Among pairs that showed a significant correlation between lncRNA and miRNA expression levels, we identified 11 lncRNA–miRNA relationships suggestive of a novel ceRNA network with relevance in MM. |
format | Online Article Text |
id | pubmed-5192483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-51924832016-12-30 In Silico Characterization of miRNA and Long Non-Coding RNA Interplay in Multiple Myeloma Ronchetti, Domenica Manzoni, Martina Todoerti, Katia Neri, Antonino Agnelli, Luca Genes (Basel) Article The identification of deregulated microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in multiple myeloma (MM) has progressively added a further level of complexity to MM biology. In addition, the cross-regulation between lncRNAs and miRNAs has begun to emerge, and theoretical and experimental studies have demonstrated the competing endogenous RNA (ceRNA) activity of lncRNAs as natural miRNA decoys in pathophysiological conditions, including cancer. Currently, information concerning lncRNA and miRNA interplay in MM is virtually absent. Herein, we investigated in silico the lncRNA and miRNA relationship in a representative datasets encompassing 95 MM and 30 plasma cell leukemia patients at diagnosis and in four normal controls, whose expression profiles were generated by a custom annotation pipeline to detect specific lncRNAs. We applied target prediction analysis based on miRanda and RNA22 algorithms to 235 lncRNAs and 459 miRNAs selected with a potential pivotal role in the pathology of MM. Among pairs that showed a significant correlation between lncRNA and miRNA expression levels, we identified 11 lncRNA–miRNA relationships suggestive of a novel ceRNA network with relevance in MM. MDPI 2016-11-29 /pmc/articles/PMC5192483/ /pubmed/27916857 http://dx.doi.org/10.3390/genes7120107 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ronchetti, Domenica Manzoni, Martina Todoerti, Katia Neri, Antonino Agnelli, Luca In Silico Characterization of miRNA and Long Non-Coding RNA Interplay in Multiple Myeloma |
title | In Silico Characterization of miRNA and Long Non-Coding RNA Interplay in Multiple Myeloma |
title_full | In Silico Characterization of miRNA and Long Non-Coding RNA Interplay in Multiple Myeloma |
title_fullStr | In Silico Characterization of miRNA and Long Non-Coding RNA Interplay in Multiple Myeloma |
title_full_unstemmed | In Silico Characterization of miRNA and Long Non-Coding RNA Interplay in Multiple Myeloma |
title_short | In Silico Characterization of miRNA and Long Non-Coding RNA Interplay in Multiple Myeloma |
title_sort | in silico characterization of mirna and long non-coding rna interplay in multiple myeloma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192483/ https://www.ncbi.nlm.nih.gov/pubmed/27916857 http://dx.doi.org/10.3390/genes7120107 |
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