Stem-Loop RT-qPCR as an Efficient Tool for the Detection and Quantification of Small RNAs in Giardia lamblia

Stem-loop quantitative reverse transcription PCR (RT-qPCR) is a molecular technique used for identification and quantification of individual small RNAs in cells. In this work, we used a Universal ProbeLibrary (UPL)-based design to detect—in a rapid, sensitive, specific, and reproducible way—the smal...

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Detalles Bibliográficos
Autores principales: Marcial-Quino, Jaime, Gómez-Manzo, Saúl, Fierro, Francisco, Vanoye-Carlo, America, Rufino-González, Yadira, Sierra-Palacios, Edgar, Castillo-Villanueva, Adriana, Castillo-Rodríguez, Rosa Angélica, Rodríguez-Bustamante, Eduardo, Arreguin-Espinosa, Roberto, Reyes-Vivas, Horacio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192507/
https://www.ncbi.nlm.nih.gov/pubmed/27999395
http://dx.doi.org/10.3390/genes7120131

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