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Oral delivery of dsRNA by microbes: Beyond pest control

RNA interference (RNAi) by oral delivery of dsRNA in insects has great potential as a tool for integrated pest management (IPM), especially with respect to addressing the need to reduce off-target effect and slow down resistance development to chemical insecticides. Employing the natural association...

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Detalles Bibliográficos
Autores principales: Abrieux, Antoine, Chiu, Joanna C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5193050/
https://www.ncbi.nlm.nih.gov/pubmed/28042376
http://dx.doi.org/10.1080/19420889.2016.1236163
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author Abrieux, Antoine
Chiu, Joanna C.
author_facet Abrieux, Antoine
Chiu, Joanna C.
author_sort Abrieux, Antoine
collection PubMed
description RNA interference (RNAi) by oral delivery of dsRNA in insects has great potential as a tool for integrated pest management (IPM), especially with respect to addressing the need to reduce off-target effect and slow down resistance development to chemical insecticides. Employing the natural association existing between insect and yeast, we developed a novel method to enable the knock down of vital genes in the pest insect Drosophila suzukii through oral delivery of species-specific dsRNA using genetically modified Saccharomyces cerevisae. D. suzukii that were fed with our “yeast biopesticide” showed a significant decrease in fitness. In this perspective article, we postulate that this approach could be adapted to a large number of species, given the great diversity of symbiotic interactions involving microorganisms and host species. Furthermore, we speculate that beyond its application as biopesticide, dsRNA delivery by genetically modified microbes can also serve to facilitate reverse genetic applications, specifically in non-model organisms.
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spelling pubmed-51930502016-12-30 Oral delivery of dsRNA by microbes: Beyond pest control Abrieux, Antoine Chiu, Joanna C. Commun Integr Biol Article Addendum RNA interference (RNAi) by oral delivery of dsRNA in insects has great potential as a tool for integrated pest management (IPM), especially with respect to addressing the need to reduce off-target effect and slow down resistance development to chemical insecticides. Employing the natural association existing between insect and yeast, we developed a novel method to enable the knock down of vital genes in the pest insect Drosophila suzukii through oral delivery of species-specific dsRNA using genetically modified Saccharomyces cerevisae. D. suzukii that were fed with our “yeast biopesticide” showed a significant decrease in fitness. In this perspective article, we postulate that this approach could be adapted to a large number of species, given the great diversity of symbiotic interactions involving microorganisms and host species. Furthermore, we speculate that beyond its application as biopesticide, dsRNA delivery by genetically modified microbes can also serve to facilitate reverse genetic applications, specifically in non-model organisms. Taylor & Francis 2016-11-04 /pmc/articles/PMC5193050/ /pubmed/28042376 http://dx.doi.org/10.1080/19420889.2016.1236163 Text en © 2016 The Author(s). Published with license by Taylor & Francis. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Article Addendum
Abrieux, Antoine
Chiu, Joanna C.
Oral delivery of dsRNA by microbes: Beyond pest control
title Oral delivery of dsRNA by microbes: Beyond pest control
title_full Oral delivery of dsRNA by microbes: Beyond pest control
title_fullStr Oral delivery of dsRNA by microbes: Beyond pest control
title_full_unstemmed Oral delivery of dsRNA by microbes: Beyond pest control
title_short Oral delivery of dsRNA by microbes: Beyond pest control
title_sort oral delivery of dsrna by microbes: beyond pest control
topic Article Addendum
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5193050/
https://www.ncbi.nlm.nih.gov/pubmed/28042376
http://dx.doi.org/10.1080/19420889.2016.1236163
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