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Effects of Propidium Monoazide (PMA) Treatment on Mycobiome and Bacteriome Analysis of Cystic Fibrosis Airways during Exacerbation

INTRODUCTION AND PURPOSE: Propidium monoazide (PMA)-pretreatment has increasingly been applied to remove the bias from dead or damaged cell artefacts, which could impact the microbiota analysis by high-throughput sequencing. Our study aimed to determine whether a PMA-pretreatment coupled with high-t...

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Detalles Bibliográficos
Autores principales: Nguyen, Linh Do Ngoc, Deschaght, Pieter, Merlin, Sophie, Loywick, Alexandre, Audebert, Christophe, Van Daele, Sabine, Viscogliosi, Eric, Vaneechoutte, Mario, Delhaes, Laurence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5193350/
https://www.ncbi.nlm.nih.gov/pubmed/28030619
http://dx.doi.org/10.1371/journal.pone.0168860