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No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population

OBJECTIVES: High-risk human papillomavirus (hrHPV) is known to be a representative cancer-causing agent in the genital and head and neck regions. Many studies have detected hrHPV DNA in nonsmall cell lung carcinoma. However, hrHPV–etiologic correlation in nonsmall cell lung carcinoma remains unclear...

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Autores principales: Lee, Jeong Eun, Lee, Yong-Moon, Seong, In-Ock, Kang, Min-Woong, Lee, Choong Sik, Kim, Kyung-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korea Centers for Disease Control and Prevention 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5194223/
https://www.ncbi.nlm.nih.gov/pubmed/28053840
http://dx.doi.org/10.1016/j.phrp.2016.10.004
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author Lee, Jeong Eun
Lee, Yong-Moon
Seong, In-Ock
Kang, Min-Woong
Lee, Choong Sik
Kim, Kyung-Hee
author_facet Lee, Jeong Eun
Lee, Yong-Moon
Seong, In-Ock
Kang, Min-Woong
Lee, Choong Sik
Kim, Kyung-Hee
author_sort Lee, Jeong Eun
collection PubMed
description OBJECTIVES: High-risk human papillomavirus (hrHPV) is known to be a representative cancer-causing agent in the genital and head and neck regions. Many studies have detected hrHPV DNA in nonsmall cell lung carcinoma. However, hrHPV–etiologic correlation in nonsmall cell lung carcinoma remains unclear. This study is designed to determine the prevalence of episomal or integrated hrHPV DNA in nonsmall cell lung carcinoma among the Korean population. METHODS: Surgically resected nonsmall cell lung carcinoma tissues, including 134 cases of squamous cell carcinoma (SqCC) and 99 cases of adenocarcinoma (ADC), were examined. In situ hybridization (ISH) for detecting episomal or integrated hrHPV DNA was performed using the INFORM HPV III Family 16 Probe (B) in the Ventana-validated assay. Anyplex II HPV28 detection kit based on real-time polymerase chain reaction was used for HPV DNA detection and genotyping. RESULTS: All members of the study population were of Korean ethnicity. Episomal or integrated hrHPV DNA ISH analysis result was negative in all 233 cases. One SqCC of 89 samples (42 SqCCs and 47 ADCs) was positive for an hrHPV genotype by Anyplex II HPV28 detection kit. CONCLUSION: Our finding did not demonstrate hrHPV–etiologic correlation in primary lung SqCC and ADC in the Korean population.
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spelling pubmed-51942232017-01-04 No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population Lee, Jeong Eun Lee, Yong-Moon Seong, In-Ock Kang, Min-Woong Lee, Choong Sik Kim, Kyung-Hee Osong Public Health Res Perspect Original Article OBJECTIVES: High-risk human papillomavirus (hrHPV) is known to be a representative cancer-causing agent in the genital and head and neck regions. Many studies have detected hrHPV DNA in nonsmall cell lung carcinoma. However, hrHPV–etiologic correlation in nonsmall cell lung carcinoma remains unclear. This study is designed to determine the prevalence of episomal or integrated hrHPV DNA in nonsmall cell lung carcinoma among the Korean population. METHODS: Surgically resected nonsmall cell lung carcinoma tissues, including 134 cases of squamous cell carcinoma (SqCC) and 99 cases of adenocarcinoma (ADC), were examined. In situ hybridization (ISH) for detecting episomal or integrated hrHPV DNA was performed using the INFORM HPV III Family 16 Probe (B) in the Ventana-validated assay. Anyplex II HPV28 detection kit based on real-time polymerase chain reaction was used for HPV DNA detection and genotyping. RESULTS: All members of the study population were of Korean ethnicity. Episomal or integrated hrHPV DNA ISH analysis result was negative in all 233 cases. One SqCC of 89 samples (42 SqCCs and 47 ADCs) was positive for an hrHPV genotype by Anyplex II HPV28 detection kit. CONCLUSION: Our finding did not demonstrate hrHPV–etiologic correlation in primary lung SqCC and ADC in the Korean population. Korea Centers for Disease Control and Prevention 2016-12 2016-10-21 /pmc/articles/PMC5194223/ /pubmed/28053840 http://dx.doi.org/10.1016/j.phrp.2016.10.004 Text en Copyright © 2016 Korea Centers for Disease Control and Prevention. Published by Elsevier Korea LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Lee, Jeong Eun
Lee, Yong-Moon
Seong, In-Ock
Kang, Min-Woong
Lee, Choong Sik
Kim, Kyung-Hee
No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population
title No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population
title_full No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population
title_fullStr No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population
title_full_unstemmed No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population
title_short No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population
title_sort no detection of episomal or integrated high-risk human papillomavirus in nonsmall cell lung carcinomas among korean population
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5194223/
https://www.ncbi.nlm.nih.gov/pubmed/28053840
http://dx.doi.org/10.1016/j.phrp.2016.10.004
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