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Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants

Archaea, like bacteria, use type IV pili to facilitate surface adhesion. Moreover, archaeal flagella—structures required for motility—share a common ancestry with type IV pili. While the characterization of archaeal homologs of bacterial type IV pilus biosynthesis components has revealed important a...

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Autores principales: Legerme, Georgio, Yang, Evan, Esquivel, Rianne N., Kiljunen, Saija, Savilahti, Harri, Pohlschroder, Mechthild
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5198076/
https://www.ncbi.nlm.nih.gov/pubmed/27898036
http://dx.doi.org/10.3390/life6040041
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author Legerme, Georgio
Yang, Evan
Esquivel, Rianne N.
Kiljunen, Saija
Savilahti, Harri
Pohlschroder, Mechthild
author_facet Legerme, Georgio
Yang, Evan
Esquivel, Rianne N.
Kiljunen, Saija
Savilahti, Harri
Pohlschroder, Mechthild
author_sort Legerme, Georgio
collection PubMed
description Archaea, like bacteria, use type IV pili to facilitate surface adhesion. Moreover, archaeal flagella—structures required for motility—share a common ancestry with type IV pili. While the characterization of archaeal homologs of bacterial type IV pilus biosynthesis components has revealed important aspects of flagellum and pilus biosynthesis and the mechanisms regulating motility and adhesion in archaea, many questions remain. Therefore, we screened a Haloferax volcanii transposon insertion library for motility mutants using motility plates and adhesion mutants, using an adapted air–liquid interface assay. Here, we identify 20 genes, previously unknown to affect motility or adhesion. These genes include potential novel regulatory genes that will help to unravel the mechanisms underpinning these processes. Both screens also identified distinct insertions within the genomic region lying between two chemotaxis genes, suggesting that chemotaxis not only plays a role in archaeal motility, but also in adhesion. Studying these genes, as well as hypothetical genes hvo_2512 and hvo_2876—also critical for both motility and adhesion—will likely elucidate how these two systems interact. Furthermore, this study underscores the usefulness of the transposon library to screen other archaeal cellular processes for specific phenotypic defects.
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spelling pubmed-51980762017-01-04 Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants Legerme, Georgio Yang, Evan Esquivel, Rianne N. Kiljunen, Saija Savilahti, Harri Pohlschroder, Mechthild Life (Basel) Article Archaea, like bacteria, use type IV pili to facilitate surface adhesion. Moreover, archaeal flagella—structures required for motility—share a common ancestry with type IV pili. While the characterization of archaeal homologs of bacterial type IV pilus biosynthesis components has revealed important aspects of flagellum and pilus biosynthesis and the mechanisms regulating motility and adhesion in archaea, many questions remain. Therefore, we screened a Haloferax volcanii transposon insertion library for motility mutants using motility plates and adhesion mutants, using an adapted air–liquid interface assay. Here, we identify 20 genes, previously unknown to affect motility or adhesion. These genes include potential novel regulatory genes that will help to unravel the mechanisms underpinning these processes. Both screens also identified distinct insertions within the genomic region lying between two chemotaxis genes, suggesting that chemotaxis not only plays a role in archaeal motility, but also in adhesion. Studying these genes, as well as hypothetical genes hvo_2512 and hvo_2876—also critical for both motility and adhesion—will likely elucidate how these two systems interact. Furthermore, this study underscores the usefulness of the transposon library to screen other archaeal cellular processes for specific phenotypic defects. MDPI 2016-11-26 /pmc/articles/PMC5198076/ /pubmed/27898036 http://dx.doi.org/10.3390/life6040041 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Legerme, Georgio
Yang, Evan
Esquivel, Rianne N.
Kiljunen, Saija
Savilahti, Harri
Pohlschroder, Mechthild
Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants
title Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants
title_full Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants
title_fullStr Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants
title_full_unstemmed Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants
title_short Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants
title_sort screening of a haloferax volcanii transposon library reveals novel motility and adhesion mutants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5198076/
https://www.ncbi.nlm.nih.gov/pubmed/27898036
http://dx.doi.org/10.3390/life6040041
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