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Comparison of Model Predictions and Laboratory Observations of Transgene Frequencies in Continuously-Breeding Mosquito Populations

The persistence of transgenes in the environment is a consideration in risk assessments of transgenic organisms. Combining mathematical models that predict the frequency of transgenes and experimental demonstrations can validate the model predictions, or can detect significant biological deviations...

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Detalles Bibliográficos
Autores principales: Valerio, Laura, North, Ace, Collins, C. Matilda, Mumford, John D., Facchinelli, Luca, Spaccapelo, Roberta, Benedict, Mark Q.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5198195/
https://www.ncbi.nlm.nih.gov/pubmed/27669312
http://dx.doi.org/10.3390/insects7040047
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author Valerio, Laura
North, Ace
Collins, C. Matilda
Mumford, John D.
Facchinelli, Luca
Spaccapelo, Roberta
Benedict, Mark Q.
author_facet Valerio, Laura
North, Ace
Collins, C. Matilda
Mumford, John D.
Facchinelli, Luca
Spaccapelo, Roberta
Benedict, Mark Q.
author_sort Valerio, Laura
collection PubMed
description The persistence of transgenes in the environment is a consideration in risk assessments of transgenic organisms. Combining mathematical models that predict the frequency of transgenes and experimental demonstrations can validate the model predictions, or can detect significant biological deviations that were neither apparent nor included as model parameters. In order to assess the correlation between predictions and observations, models were constructed to estimate the frequency of a transgene causing male sexual sterility in simulated populations of a malaria mosquito Anopheles gambiae that were seeded with transgenic females at various proportions. Concurrently, overlapping-generation laboratory populations similar to those being modeled were initialized with various starting transgene proportions, and the subsequent proportions of transgenic individuals in populations were determined weekly until the transgene disappeared. The specific transgene being tested contained a homing endonuclease gene expressed in testes, I-PpoI, that cleaves the ribosomal DNA and results in complete male sexual sterility with no effect on female fertility. The transgene was observed to disappear more rapidly than the model predicted in all cases. The period before ovipositions that contained no transgenic progeny ranged from as little as three weeks after cage initiation to as long as 11 weeks.
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spelling pubmed-51981952017-01-04 Comparison of Model Predictions and Laboratory Observations of Transgene Frequencies in Continuously-Breeding Mosquito Populations Valerio, Laura North, Ace Collins, C. Matilda Mumford, John D. Facchinelli, Luca Spaccapelo, Roberta Benedict, Mark Q. Insects Article The persistence of transgenes in the environment is a consideration in risk assessments of transgenic organisms. Combining mathematical models that predict the frequency of transgenes and experimental demonstrations can validate the model predictions, or can detect significant biological deviations that were neither apparent nor included as model parameters. In order to assess the correlation between predictions and observations, models were constructed to estimate the frequency of a transgene causing male sexual sterility in simulated populations of a malaria mosquito Anopheles gambiae that were seeded with transgenic females at various proportions. Concurrently, overlapping-generation laboratory populations similar to those being modeled were initialized with various starting transgene proportions, and the subsequent proportions of transgenic individuals in populations were determined weekly until the transgene disappeared. The specific transgene being tested contained a homing endonuclease gene expressed in testes, I-PpoI, that cleaves the ribosomal DNA and results in complete male sexual sterility with no effect on female fertility. The transgene was observed to disappear more rapidly than the model predicted in all cases. The period before ovipositions that contained no transgenic progeny ranged from as little as three weeks after cage initiation to as long as 11 weeks. MDPI 2016-09-22 /pmc/articles/PMC5198195/ /pubmed/27669312 http://dx.doi.org/10.3390/insects7040047 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Valerio, Laura
North, Ace
Collins, C. Matilda
Mumford, John D.
Facchinelli, Luca
Spaccapelo, Roberta
Benedict, Mark Q.
Comparison of Model Predictions and Laboratory Observations of Transgene Frequencies in Continuously-Breeding Mosquito Populations
title Comparison of Model Predictions and Laboratory Observations of Transgene Frequencies in Continuously-Breeding Mosquito Populations
title_full Comparison of Model Predictions and Laboratory Observations of Transgene Frequencies in Continuously-Breeding Mosquito Populations
title_fullStr Comparison of Model Predictions and Laboratory Observations of Transgene Frequencies in Continuously-Breeding Mosquito Populations
title_full_unstemmed Comparison of Model Predictions and Laboratory Observations of Transgene Frequencies in Continuously-Breeding Mosquito Populations
title_short Comparison of Model Predictions and Laboratory Observations of Transgene Frequencies in Continuously-Breeding Mosquito Populations
title_sort comparison of model predictions and laboratory observations of transgene frequencies in continuously-breeding mosquito populations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5198195/
https://www.ncbi.nlm.nih.gov/pubmed/27669312
http://dx.doi.org/10.3390/insects7040047
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