Quantifying western blots: none more black

Western blotting is among the most common techniques used in molecular biology and a simple way of assessing the presence or absence of a protein. It is also commonly used to compare protein levels in different conditions or in different tissues. This article illustrates some of the easy ways to arr...

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Detalles Bibliográficos
Autor principal: Bell, Graham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Comment
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5198504/
https://www.ncbi.nlm.nih.gov/pubmed/28031029
http://dx.doi.org/10.1186/s12915-016-0339-1
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author Bell, Graham
author_facet Bell, Graham
author_sort Bell, Graham
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description Western blotting is among the most common techniques used in molecular biology and a simple way of assessing the presence or absence of a protein. It is also commonly used to compare protein levels in different conditions or in different tissues. This article illustrates some of the easy ways to arrive at a false conclusion when trying to quantify protein levels from western blots.
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spelling pubmed-51985042016-12-30 Quantifying western blots: none more black Bell, Graham BMC Biol Comment Western blotting is among the most common techniques used in molecular biology and a simple way of assessing the presence or absence of a protein. It is also commonly used to compare protein levels in different conditions or in different tissues. This article illustrates some of the easy ways to arrive at a false conclusion when trying to quantify protein levels from western blots. BioMed Central 2016-12-28 /pmc/articles/PMC5198504/ /pubmed/28031029 http://dx.doi.org/10.1186/s12915-016-0339-1 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Comment
Bell, Graham
Quantifying western blots: none more black
title Quantifying western blots: none more black
title_full Quantifying western blots: none more black
title_fullStr Quantifying western blots: none more black
title_full_unstemmed Quantifying western blots: none more black
title_short Quantifying western blots: none more black
title_sort quantifying western blots: none more black
topic Comment
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5198504/
https://www.ncbi.nlm.nih.gov/pubmed/28031029
http://dx.doi.org/10.1186/s12915-016-0339-1
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