Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer

In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells...

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Detalles Bibliográficos
Autores principales: Shramova, E.I., Proshkina, G.M., Chumakov, S.P., Khodarovich, Yu.M., Deyev, S.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5199213/
https://www.ncbi.nlm.nih.gov/pubmed/28050273
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author Shramova, E.I.
Proshkina, G.M.
Chumakov, S.P.
Khodarovich, Yu.M.
Deyev, S.M.
author_facet Shramova, E.I.
Proshkina, G.M.
Chumakov, S.P.
Khodarovich, Yu.M.
Deyev, S.M.
author_sort Shramova, E.I.
collection PubMed
description In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the presence of its substrate, furimazine. Upon such condition, miniSOG possesses photoinduced cytotoxicity and causes a 48% cell death level in a stably transfected cell line.
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spelling pubmed-51992132017-01-03 Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer Shramova, E.I. Proshkina, G.M. Chumakov, S.P. Khodarovich, Yu.M. Deyev, S.M. Acta Naturae Research Article In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the presence of its substrate, furimazine. Upon such condition, miniSOG possesses photoinduced cytotoxicity and causes a 48% cell death level in a stably transfected cell line. A.I. Gordeyev 2016 /pmc/articles/PMC5199213/ /pubmed/28050273 Text en Copyright ® 2016 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shramova, E.I.
Proshkina, G.M.
Chumakov, S.P.
Khodarovich, Yu.M.
Deyev, S.M.
Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer
title Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer
title_full Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer
title_fullStr Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer
title_full_unstemmed Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer
title_short Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer
title_sort flavoprotein minisog cytotoxisity can be induced by bioluminescence resonance energy transfer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5199213/
https://www.ncbi.nlm.nih.gov/pubmed/28050273
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AT khodarovichyum flavoproteinminisogcytotoxisitycanbeinducedbybioluminescenceresonanceenergytransfer
AT deyevsm flavoproteinminisogcytotoxisitycanbeinducedbybioluminescenceresonanceenergytransfer

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