Cargando…
De novo transcriptome assembly of shrimp Palaemon serratus
The shrimp Palaemon serratus is a coastal decapod crustacean with a high commercial value. It is harvested for human consumption. In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of P. serratus. RNA was isolated from muscle of ad...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5200869/ https://www.ncbi.nlm.nih.gov/pubmed/28066712 http://dx.doi.org/10.1016/j.gdata.2016.12.009 |
_version_ | 1782489256304836608 |
---|---|
author | Perina, Alejandra González-Tizón, Ana M Meilán, Iago F. Martínez-Lage, Andrés |
author_facet | Perina, Alejandra González-Tizón, Ana M Meilán, Iago F. Martínez-Lage, Andrés |
author_sort | Perina, Alejandra |
collection | PubMed |
description | The shrimp Palaemon serratus is a coastal decapod crustacean with a high commercial value. It is harvested for human consumption. In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of P. serratus. RNA was isolated from muscle of adults individuals and, from a pool of larvae. A total number of 4 cDNA libraries were constructed, using the TruSeq RNA Sample Preparation Kit v2. The raw data in this study was deposited in NCBI SRA database with study accession number of SRP090769. The obtained data were subjected to de novo transcriptome assembly using Trinity software, and coding regions were predicted by TransDecoder. We used Blastp and Sma3s to annotate the identified proteins. The transcriptome data could provide some insight into the understanding of genes involved in the larval development and metamorphosis. SPECIFICATIONS: |
format | Online Article Text |
id | pubmed-5200869 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-52008692017-01-06 De novo transcriptome assembly of shrimp Palaemon serratus Perina, Alejandra González-Tizón, Ana M Meilán, Iago F. Martínez-Lage, Andrés Genom Data Data in Brief Article The shrimp Palaemon serratus is a coastal decapod crustacean with a high commercial value. It is harvested for human consumption. In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of P. serratus. RNA was isolated from muscle of adults individuals and, from a pool of larvae. A total number of 4 cDNA libraries were constructed, using the TruSeq RNA Sample Preparation Kit v2. The raw data in this study was deposited in NCBI SRA database with study accession number of SRP090769. The obtained data were subjected to de novo transcriptome assembly using Trinity software, and coding regions were predicted by TransDecoder. We used Blastp and Sma3s to annotate the identified proteins. The transcriptome data could provide some insight into the understanding of genes involved in the larval development and metamorphosis. SPECIFICATIONS: Elsevier 2016-12-23 /pmc/articles/PMC5200869/ /pubmed/28066712 http://dx.doi.org/10.1016/j.gdata.2016.12.009 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data in Brief Article Perina, Alejandra González-Tizón, Ana M Meilán, Iago F. Martínez-Lage, Andrés De novo transcriptome assembly of shrimp Palaemon serratus |
title | De novo transcriptome assembly of shrimp Palaemon serratus |
title_full | De novo transcriptome assembly of shrimp Palaemon serratus |
title_fullStr | De novo transcriptome assembly of shrimp Palaemon serratus |
title_full_unstemmed | De novo transcriptome assembly of shrimp Palaemon serratus |
title_short | De novo transcriptome assembly of shrimp Palaemon serratus |
title_sort | de novo transcriptome assembly of shrimp palaemon serratus |
topic | Data in Brief Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5200869/ https://www.ncbi.nlm.nih.gov/pubmed/28066712 http://dx.doi.org/10.1016/j.gdata.2016.12.009 |
work_keys_str_mv | AT perinaalejandra denovotranscriptomeassemblyofshrimppalaemonserratus AT gonzaleztizonanam denovotranscriptomeassemblyofshrimppalaemonserratus AT meilaniagof denovotranscriptomeassemblyofshrimppalaemonserratus AT martinezlageandres denovotranscriptomeassemblyofshrimppalaemonserratus |