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De novo transcriptome assembly of shrimp Palaemon serratus

The shrimp Palaemon serratus is a coastal decapod crustacean with a high commercial value. It is harvested for human consumption. In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of P. serratus. RNA was isolated from muscle of ad...

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Autores principales: Perina, Alejandra, González-Tizón, Ana M, Meilán, Iago F., Martínez-Lage, Andrés
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5200869/
https://www.ncbi.nlm.nih.gov/pubmed/28066712
http://dx.doi.org/10.1016/j.gdata.2016.12.009
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author Perina, Alejandra
González-Tizón, Ana M
Meilán, Iago F.
Martínez-Lage, Andrés
author_facet Perina, Alejandra
González-Tizón, Ana M
Meilán, Iago F.
Martínez-Lage, Andrés
author_sort Perina, Alejandra
collection PubMed
description The shrimp Palaemon serratus is a coastal decapod crustacean with a high commercial value. It is harvested for human consumption. In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of P. serratus. RNA was isolated from muscle of adults individuals and, from a pool of larvae. A total number of 4 cDNA libraries were constructed, using the TruSeq RNA Sample Preparation Kit v2. The raw data in this study was deposited in NCBI SRA database with study accession number of SRP090769. The obtained data were subjected to de novo transcriptome assembly using Trinity software, and coding regions were predicted by TransDecoder. We used Blastp and Sma3s to annotate the identified proteins. The transcriptome data could provide some insight into the understanding of genes involved in the larval development and metamorphosis. SPECIFICATIONS:
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spelling pubmed-52008692017-01-06 De novo transcriptome assembly of shrimp Palaemon serratus Perina, Alejandra González-Tizón, Ana M Meilán, Iago F. Martínez-Lage, Andrés Genom Data Data in Brief Article The shrimp Palaemon serratus is a coastal decapod crustacean with a high commercial value. It is harvested for human consumption. In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of P. serratus. RNA was isolated from muscle of adults individuals and, from a pool of larvae. A total number of 4 cDNA libraries were constructed, using the TruSeq RNA Sample Preparation Kit v2. The raw data in this study was deposited in NCBI SRA database with study accession number of SRP090769. The obtained data were subjected to de novo transcriptome assembly using Trinity software, and coding regions were predicted by TransDecoder. We used Blastp and Sma3s to annotate the identified proteins. The transcriptome data could provide some insight into the understanding of genes involved in the larval development and metamorphosis. SPECIFICATIONS: Elsevier 2016-12-23 /pmc/articles/PMC5200869/ /pubmed/28066712 http://dx.doi.org/10.1016/j.gdata.2016.12.009 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data in Brief Article
Perina, Alejandra
González-Tizón, Ana M
Meilán, Iago F.
Martínez-Lage, Andrés
De novo transcriptome assembly of shrimp Palaemon serratus
title De novo transcriptome assembly of shrimp Palaemon serratus
title_full De novo transcriptome assembly of shrimp Palaemon serratus
title_fullStr De novo transcriptome assembly of shrimp Palaemon serratus
title_full_unstemmed De novo transcriptome assembly of shrimp Palaemon serratus
title_short De novo transcriptome assembly of shrimp Palaemon serratus
title_sort de novo transcriptome assembly of shrimp palaemon serratus
topic Data in Brief Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5200869/
https://www.ncbi.nlm.nih.gov/pubmed/28066712
http://dx.doi.org/10.1016/j.gdata.2016.12.009
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