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Elimination of a Free Cysteine by Creation of a Disulfide Bond Increases the Activity and Stability of Candida boidinii Formate Dehydrogenase

NAD(+)-dependent formate dehydrogenase (FDH; EC 1.2.1.2) is an industrial enzyme widely used for NADH regeneration. However, enzyme inactivation caused by the oxidation of cysteine residues is a flaw of native FDH. In this study, we relieved the oxidation of the free cysteine of FDH from Candida boi...

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Detalles Bibliográficos
Autores principales: Zheng, Junxian, Yang, Taowei, Zhou, Junping, Xu, Meijuan, Zhang, Xian, Rao, Zhiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5203636/
https://www.ncbi.nlm.nih.gov/pubmed/27836850
http://dx.doi.org/10.1128/AEM.02624-16

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