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Maize Cytolines Unmask Key Nuclear Genes That Are under the Control of Retrograde Signaling Pathways in Plants

The genomes of the two plant organelles encode for a relatively small number of proteins. Thus, nuclear genes encode the vast majority of their proteome. Organelle-to-nucleus communication takes place through retrograde signaling (RS) pathways. Signals relayed through RS pathways have an impact on n...

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Autores principales: Miclaus, Mihai, Balacescu, Ovidiu, Has, Ioan, Balacescu, Loredana, Has, Voichita, Suteu, Dana, Neuenschwander, Samuel, Keller, Irene, Bruggmann, Rémy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5203784/
https://www.ncbi.nlm.nih.gov/pubmed/27702813
http://dx.doi.org/10.1093/gbe/evw245
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author Miclaus, Mihai
Balacescu, Ovidiu
Has, Ioan
Balacescu, Loredana
Has, Voichita
Suteu, Dana
Neuenschwander, Samuel
Keller, Irene
Bruggmann, Rémy
author_facet Miclaus, Mihai
Balacescu, Ovidiu
Has, Ioan
Balacescu, Loredana
Has, Voichita
Suteu, Dana
Neuenschwander, Samuel
Keller, Irene
Bruggmann, Rémy
author_sort Miclaus, Mihai
collection PubMed
description The genomes of the two plant organelles encode for a relatively small number of proteins. Thus, nuclear genes encode the vast majority of their proteome. Organelle-to-nucleus communication takes place through retrograde signaling (RS) pathways. Signals relayed through RS pathways have an impact on nuclear gene expression but their target-genes remain elusive in a normal state of the cell (considering that only mutants and stress have been used so far). Here, we use maize cytolines as an alternative. The nucleus of a donor line was transferred into two other cytoplasmic environments through at least nine back-crosses, in a time-span of > 10 years. The transcriptomes of the resulting cytolines were sequenced and compared. There are 96 differentially regulated nuclear genes in two cytoplasm-donor lines when compared with their nucleus-donor. They are expressed throughout plant development, in various tissues and organs. One-third of the 96 proteins have a human homolog, stressing their potential role in mitochondrial RS. We also identified syntenic orthologous genes in four other grasses and homologous genes in Arabidopsis thaliana. These findings contribute to the paradigm we use to describe the RS in plants. The 96 nuclear genes identified here are not differentially regulated as a result of mutation, or any kind of stress. They are rather key players of the organelle-to-nucleus communication in a normal state of the cell.
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spelling pubmed-52037842017-01-06 Maize Cytolines Unmask Key Nuclear Genes That Are under the Control of Retrograde Signaling Pathways in Plants Miclaus, Mihai Balacescu, Ovidiu Has, Ioan Balacescu, Loredana Has, Voichita Suteu, Dana Neuenschwander, Samuel Keller, Irene Bruggmann, Rémy Genome Biol Evol Research Article The genomes of the two plant organelles encode for a relatively small number of proteins. Thus, nuclear genes encode the vast majority of their proteome. Organelle-to-nucleus communication takes place through retrograde signaling (RS) pathways. Signals relayed through RS pathways have an impact on nuclear gene expression but their target-genes remain elusive in a normal state of the cell (considering that only mutants and stress have been used so far). Here, we use maize cytolines as an alternative. The nucleus of a donor line was transferred into two other cytoplasmic environments through at least nine back-crosses, in a time-span of > 10 years. The transcriptomes of the resulting cytolines were sequenced and compared. There are 96 differentially regulated nuclear genes in two cytoplasm-donor lines when compared with their nucleus-donor. They are expressed throughout plant development, in various tissues and organs. One-third of the 96 proteins have a human homolog, stressing their potential role in mitochondrial RS. We also identified syntenic orthologous genes in four other grasses and homologous genes in Arabidopsis thaliana. These findings contribute to the paradigm we use to describe the RS in plants. The 96 nuclear genes identified here are not differentially regulated as a result of mutation, or any kind of stress. They are rather key players of the organelle-to-nucleus communication in a normal state of the cell. Oxford University Press 2016-10-03 /pmc/articles/PMC5203784/ /pubmed/27702813 http://dx.doi.org/10.1093/gbe/evw245 Text en © The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Miclaus, Mihai
Balacescu, Ovidiu
Has, Ioan
Balacescu, Loredana
Has, Voichita
Suteu, Dana
Neuenschwander, Samuel
Keller, Irene
Bruggmann, Rémy
Maize Cytolines Unmask Key Nuclear Genes That Are under the Control of Retrograde Signaling Pathways in Plants
title Maize Cytolines Unmask Key Nuclear Genes That Are under the Control of Retrograde Signaling Pathways in Plants
title_full Maize Cytolines Unmask Key Nuclear Genes That Are under the Control of Retrograde Signaling Pathways in Plants
title_fullStr Maize Cytolines Unmask Key Nuclear Genes That Are under the Control of Retrograde Signaling Pathways in Plants
title_full_unstemmed Maize Cytolines Unmask Key Nuclear Genes That Are under the Control of Retrograde Signaling Pathways in Plants
title_short Maize Cytolines Unmask Key Nuclear Genes That Are under the Control of Retrograde Signaling Pathways in Plants
title_sort maize cytolines unmask key nuclear genes that are under the control of retrograde signaling pathways in plants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5203784/
https://www.ncbi.nlm.nih.gov/pubmed/27702813
http://dx.doi.org/10.1093/gbe/evw245
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