Cargando…

Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep

Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic a...

Descripción completa

Detalles Bibliográficos
Autores principales: Ferreira, Marina G., Duarte, Clara G., Oliveira, Maira S., Castro, Karen L. P., Teixeira, Maílson S., Reis, Lílian P. G., Zambrano, José A., Kalapothakis, Evanguedes, Michel, Ana Flávia R. M., Soto-Blanco, Benito, Chávez-Olórtegui, Carlos, Melo, Marília M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5204024/
https://www.ncbi.nlm.nih.gov/pubmed/27297422
http://dx.doi.org/10.4142/jvs.2016.17.4.467
_version_ 1782489827991617536
author Ferreira, Marina G.
Duarte, Clara G.
Oliveira, Maira S.
Castro, Karen L. P.
Teixeira, Maílson S.
Reis, Lílian P. G.
Zambrano, José A.
Kalapothakis, Evanguedes
Michel, Ana Flávia R. M.
Soto-Blanco, Benito
Chávez-Olórtegui, Carlos
Melo, Marília M.
author_facet Ferreira, Marina G.
Duarte, Clara G.
Oliveira, Maira S.
Castro, Karen L. P.
Teixeira, Maílson S.
Reis, Lílian P. G.
Zambrano, José A.
Kalapothakis, Evanguedes
Michel, Ana Flávia R. M.
Soto-Blanco, Benito
Chávez-Olórtegui, Carlos
Melo, Marília M.
author_sort Ferreira, Marina G.
collection PubMed
description Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic activities of crude and detoxified Tityus serrulatus (Ts) venom in sheep during the production of anti-scorpionic anti-venom. Sheep were categorized into three groups: G1, control, immunized with buffer only; G2, immunized with crude Ts venom; and G3, immunized with glutaraldehyde-detoxified Ts venom. All animals were subjected to clinical exams and supplementary tests. G2 sheep showed mild clinical changes, but the other groups tolerated the immunization program well. Specific antibodies generated in animals immunized with either Ts crude venom or glutaraldehyde-detoxified Ts venom recognized the crude Ts venom in both assays. To evaluate the lethality neutralization potential of the produced sera, individual serum samples were pre-incubated with Ts crude venom, then subcutaneously injected into mice. Efficient immune protection of 56.3% and 43.8% against Ts crude venom was observed in G2 and G3, respectively. Overall, the results of this study support the use of sheep and glutaraldehyde-detoxified Ts venom for alternative production of specific anti-venom.
format Online
Article
Text
id pubmed-5204024
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher The Korean Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-52040242017-01-04 Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep Ferreira, Marina G. Duarte, Clara G. Oliveira, Maira S. Castro, Karen L. P. Teixeira, Maílson S. Reis, Lílian P. G. Zambrano, José A. Kalapothakis, Evanguedes Michel, Ana Flávia R. M. Soto-Blanco, Benito Chávez-Olórtegui, Carlos Melo, Marília M. J Vet Sci Original Article Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic activities of crude and detoxified Tityus serrulatus (Ts) venom in sheep during the production of anti-scorpionic anti-venom. Sheep were categorized into three groups: G1, control, immunized with buffer only; G2, immunized with crude Ts venom; and G3, immunized with glutaraldehyde-detoxified Ts venom. All animals were subjected to clinical exams and supplementary tests. G2 sheep showed mild clinical changes, but the other groups tolerated the immunization program well. Specific antibodies generated in animals immunized with either Ts crude venom or glutaraldehyde-detoxified Ts venom recognized the crude Ts venom in both assays. To evaluate the lethality neutralization potential of the produced sera, individual serum samples were pre-incubated with Ts crude venom, then subcutaneously injected into mice. Efficient immune protection of 56.3% and 43.8% against Ts crude venom was observed in G2 and G3, respectively. Overall, the results of this study support the use of sheep and glutaraldehyde-detoxified Ts venom for alternative production of specific anti-venom. The Korean Society of Veterinary Science 2016-12 2016-12-20 /pmc/articles/PMC5204024/ /pubmed/27297422 http://dx.doi.org/10.4142/jvs.2016.17.4.467 Text en © 2016 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ferreira, Marina G.
Duarte, Clara G.
Oliveira, Maira S.
Castro, Karen L. P.
Teixeira, Maílson S.
Reis, Lílian P. G.
Zambrano, José A.
Kalapothakis, Evanguedes
Michel, Ana Flávia R. M.
Soto-Blanco, Benito
Chávez-Olórtegui, Carlos
Melo, Marília M.
Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep
title Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep
title_full Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep
title_fullStr Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep
title_full_unstemmed Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep
title_short Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep
title_sort toxicity of crude and detoxified tityus serrulatus venom in anti-venom-producing sheep
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5204024/
https://www.ncbi.nlm.nih.gov/pubmed/27297422
http://dx.doi.org/10.4142/jvs.2016.17.4.467
work_keys_str_mv AT ferreiramarinag toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT duarteclarag toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT oliveiramairas toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT castrokarenlp toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT teixeiramailsons toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT reislilianpg toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT zambranojosea toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT kalapothakisevanguedes toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT michelanaflaviarm toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT sotoblancobenito toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT chavezolorteguicarlos toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep
AT melomariliam toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep