Cargando…
Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep
Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic a...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5204024/ https://www.ncbi.nlm.nih.gov/pubmed/27297422 http://dx.doi.org/10.4142/jvs.2016.17.4.467 |
_version_ | 1782489827991617536 |
---|---|
author | Ferreira, Marina G. Duarte, Clara G. Oliveira, Maira S. Castro, Karen L. P. Teixeira, Maílson S. Reis, Lílian P. G. Zambrano, José A. Kalapothakis, Evanguedes Michel, Ana Flávia R. M. Soto-Blanco, Benito Chávez-Olórtegui, Carlos Melo, Marília M. |
author_facet | Ferreira, Marina G. Duarte, Clara G. Oliveira, Maira S. Castro, Karen L. P. Teixeira, Maílson S. Reis, Lílian P. G. Zambrano, José A. Kalapothakis, Evanguedes Michel, Ana Flávia R. M. Soto-Blanco, Benito Chávez-Olórtegui, Carlos Melo, Marília M. |
author_sort | Ferreira, Marina G. |
collection | PubMed |
description | Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic activities of crude and detoxified Tityus serrulatus (Ts) venom in sheep during the production of anti-scorpionic anti-venom. Sheep were categorized into three groups: G1, control, immunized with buffer only; G2, immunized with crude Ts venom; and G3, immunized with glutaraldehyde-detoxified Ts venom. All animals were subjected to clinical exams and supplementary tests. G2 sheep showed mild clinical changes, but the other groups tolerated the immunization program well. Specific antibodies generated in animals immunized with either Ts crude venom or glutaraldehyde-detoxified Ts venom recognized the crude Ts venom in both assays. To evaluate the lethality neutralization potential of the produced sera, individual serum samples were pre-incubated with Ts crude venom, then subcutaneously injected into mice. Efficient immune protection of 56.3% and 43.8% against Ts crude venom was observed in G2 and G3, respectively. Overall, the results of this study support the use of sheep and glutaraldehyde-detoxified Ts venom for alternative production of specific anti-venom. |
format | Online Article Text |
id | pubmed-5204024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-52040242017-01-04 Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep Ferreira, Marina G. Duarte, Clara G. Oliveira, Maira S. Castro, Karen L. P. Teixeira, Maílson S. Reis, Lílian P. G. Zambrano, José A. Kalapothakis, Evanguedes Michel, Ana Flávia R. M. Soto-Blanco, Benito Chávez-Olórtegui, Carlos Melo, Marília M. J Vet Sci Original Article Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic activities of crude and detoxified Tityus serrulatus (Ts) venom in sheep during the production of anti-scorpionic anti-venom. Sheep were categorized into three groups: G1, control, immunized with buffer only; G2, immunized with crude Ts venom; and G3, immunized with glutaraldehyde-detoxified Ts venom. All animals were subjected to clinical exams and supplementary tests. G2 sheep showed mild clinical changes, but the other groups tolerated the immunization program well. Specific antibodies generated in animals immunized with either Ts crude venom or glutaraldehyde-detoxified Ts venom recognized the crude Ts venom in both assays. To evaluate the lethality neutralization potential of the produced sera, individual serum samples were pre-incubated with Ts crude venom, then subcutaneously injected into mice. Efficient immune protection of 56.3% and 43.8% against Ts crude venom was observed in G2 and G3, respectively. Overall, the results of this study support the use of sheep and glutaraldehyde-detoxified Ts venom for alternative production of specific anti-venom. The Korean Society of Veterinary Science 2016-12 2016-12-20 /pmc/articles/PMC5204024/ /pubmed/27297422 http://dx.doi.org/10.4142/jvs.2016.17.4.467 Text en © 2016 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ferreira, Marina G. Duarte, Clara G. Oliveira, Maira S. Castro, Karen L. P. Teixeira, Maílson S. Reis, Lílian P. G. Zambrano, José A. Kalapothakis, Evanguedes Michel, Ana Flávia R. M. Soto-Blanco, Benito Chávez-Olórtegui, Carlos Melo, Marília M. Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep |
title | Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep |
title_full | Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep |
title_fullStr | Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep |
title_full_unstemmed | Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep |
title_short | Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep |
title_sort | toxicity of crude and detoxified tityus serrulatus venom in anti-venom-producing sheep |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5204024/ https://www.ncbi.nlm.nih.gov/pubmed/27297422 http://dx.doi.org/10.4142/jvs.2016.17.4.467 |
work_keys_str_mv | AT ferreiramarinag toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT duarteclarag toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT oliveiramairas toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT castrokarenlp toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT teixeiramailsons toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT reislilianpg toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT zambranojosea toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT kalapothakisevanguedes toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT michelanaflaviarm toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT sotoblancobenito toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT chavezolorteguicarlos toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep AT melomariliam toxicityofcrudeanddetoxifiedtityusserrulatusvenominantivenomproducingsheep |