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Two clinical isolates of Mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs

Mycoplasma (M.) hyosynoviae is known to colonize and cause disease in growing-finishing pigs. In this study, two clinical isolates of M. hyosynoviae were compared by inoculating cesarean-derived colostrum-deprived and specific-pathogen-free growing pigs. After intranasal or intravenous inoculation,...

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Autores principales: Gomes-Neto, João Carlos, Raymond, Matthew, Bower, Leslie, Ramirez, Alejandro, Madson, Darin M., Strait, Erin L., Rosey, Everett L., Rapp-Gabrielson, Vicki J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5204026/
https://www.ncbi.nlm.nih.gov/pubmed/27297416
http://dx.doi.org/10.4142/jvs.2016.17.4.489
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author Gomes-Neto, João Carlos
Raymond, Matthew
Bower, Leslie
Ramirez, Alejandro
Madson, Darin M.
Strait, Erin L.
Rosey, Everett L.
Rapp-Gabrielson, Vicki J.
author_facet Gomes-Neto, João Carlos
Raymond, Matthew
Bower, Leslie
Ramirez, Alejandro
Madson, Darin M.
Strait, Erin L.
Rosey, Everett L.
Rapp-Gabrielson, Vicki J.
author_sort Gomes-Neto, João Carlos
collection PubMed
description Mycoplasma (M.) hyosynoviae is known to colonize and cause disease in growing-finishing pigs. In this study, two clinical isolates of M. hyosynoviae were compared by inoculating cesarean-derived colostrum-deprived and specific-pathogen-free growing pigs. After intranasal or intravenous inoculation, the proportion and distribution pattern of clinical cases was compared in addition to the severity of lameness. Tonsils were found to be the primary site of colonization, while bacteremia was rarely detected prior to the observation of clinical signs. Regardless of the clinical isolate, route of inoculation, or volume of inocula, histopathological alterations and tissue invasion were detected in multiple joints, indicating an apparent lack of specific joint tropism. Acute disease was primarily observed 7 to 10 days post-inoculation. The variability in the severity of synovial microscopic lesions and pathogen detection in joint cavities suggests that the duration of joint infection may influence the diagnostic accuracy. In summary, these findings demonstrate that diagnosis of M. hyosynoviae-associated arthritis can be influenced by the clinical isolate, and provides a study platform to investigate the colonization and virulence potential of field isolates. This approach can be particularly relevant to auxiliate in surveillance and testing of therapeutic and/or vaccine candidates.
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spelling pubmed-52040262017-01-04 Two clinical isolates of Mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs Gomes-Neto, João Carlos Raymond, Matthew Bower, Leslie Ramirez, Alejandro Madson, Darin M. Strait, Erin L. Rosey, Everett L. Rapp-Gabrielson, Vicki J. J Vet Sci Original Article Mycoplasma (M.) hyosynoviae is known to colonize and cause disease in growing-finishing pigs. In this study, two clinical isolates of M. hyosynoviae were compared by inoculating cesarean-derived colostrum-deprived and specific-pathogen-free growing pigs. After intranasal or intravenous inoculation, the proportion and distribution pattern of clinical cases was compared in addition to the severity of lameness. Tonsils were found to be the primary site of colonization, while bacteremia was rarely detected prior to the observation of clinical signs. Regardless of the clinical isolate, route of inoculation, or volume of inocula, histopathological alterations and tissue invasion were detected in multiple joints, indicating an apparent lack of specific joint tropism. Acute disease was primarily observed 7 to 10 days post-inoculation. The variability in the severity of synovial microscopic lesions and pathogen detection in joint cavities suggests that the duration of joint infection may influence the diagnostic accuracy. In summary, these findings demonstrate that diagnosis of M. hyosynoviae-associated arthritis can be influenced by the clinical isolate, and provides a study platform to investigate the colonization and virulence potential of field isolates. This approach can be particularly relevant to auxiliate in surveillance and testing of therapeutic and/or vaccine candidates. The Korean Society of Veterinary Science 2016-12 2016-12-20 /pmc/articles/PMC5204026/ /pubmed/27297416 http://dx.doi.org/10.4142/jvs.2016.17.4.489 Text en © 2016 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Gomes-Neto, João Carlos
Raymond, Matthew
Bower, Leslie
Ramirez, Alejandro
Madson, Darin M.
Strait, Erin L.
Rosey, Everett L.
Rapp-Gabrielson, Vicki J.
Two clinical isolates of Mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs
title Two clinical isolates of Mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs
title_full Two clinical isolates of Mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs
title_fullStr Two clinical isolates of Mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs
title_full_unstemmed Two clinical isolates of Mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs
title_short Two clinical isolates of Mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs
title_sort two clinical isolates of mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5204026/
https://www.ncbi.nlm.nih.gov/pubmed/27297416
http://dx.doi.org/10.4142/jvs.2016.17.4.489
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