Quantification of Small Molecule–Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore

[Image: see text] We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Molly M., Peterson, Blake R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2016
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5204206/
https://www.ncbi.nlm.nih.gov/pubmed/28058293
http://dx.doi.org/10.1021/acsomega.6b00356

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5204206/
https://www.ncbi.nlm.nih.gov/pubmed/28058293
http://dx.doi.org/10.1021/acsomega.6b00356

Ejemplares similares