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Rab27a/Slp2-a complex is involved in Schwann cell myelination
Myelination of Schwann cells in the peripheral nervous system is an intricate process involving myelin protein trafficking. Recently, the role and mechanism of the endosomal/lysosomal system in myelin formation were emphasized. Our previous results demonstrated that a small GTPase Rab27a regulates l...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5204241/ https://www.ncbi.nlm.nih.gov/pubmed/28123429 http://dx.doi.org/10.4103/1673-5374.194755 |
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author | Su, Wen-feng Gu, Yun Wei, Zhong-ya Shen, Yun-tian Jin, Zi-han Yuan, Ying Gu, Xiao-song Chen, Gang |
author_facet | Su, Wen-feng Gu, Yun Wei, Zhong-ya Shen, Yun-tian Jin, Zi-han Yuan, Ying Gu, Xiao-song Chen, Gang |
author_sort | Su, Wen-feng |
collection | PubMed |
description | Myelination of Schwann cells in the peripheral nervous system is an intricate process involving myelin protein trafficking. Recently, the role and mechanism of the endosomal/lysosomal system in myelin formation were emphasized. Our previous results demonstrated that a small GTPase Rab27a regulates lysosomal exocytosis and myelin protein trafficking in Schwann cells. In this present study, we established a dorsal root ganglion (DRG) neuron and Schwann cell co-culture model to identify the signals associated with Rab27a during myelination. First, Slp2-a, as the Rab27a effector, was endogenously expressed in Schwann cells. Second, Rab27a expression significantly increased during Schwann cell myelination. Finally, Rab27a and Slp2-a silencing in Schwann cells not only reduced myelin protein expression, but also impaired formation of myelin-like membranes in DRG neuron and Schwann cell co-cultures. Our findings suggest that the Rab27a/Slp2-a complex affects Schwann cell myelination in vitro. |
format | Online Article Text |
id | pubmed-5204241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-52042412017-01-25 Rab27a/Slp2-a complex is involved in Schwann cell myelination Su, Wen-feng Gu, Yun Wei, Zhong-ya Shen, Yun-tian Jin, Zi-han Yuan, Ying Gu, Xiao-song Chen, Gang Neural Regen Res Research Article Myelination of Schwann cells in the peripheral nervous system is an intricate process involving myelin protein trafficking. Recently, the role and mechanism of the endosomal/lysosomal system in myelin formation were emphasized. Our previous results demonstrated that a small GTPase Rab27a regulates lysosomal exocytosis and myelin protein trafficking in Schwann cells. In this present study, we established a dorsal root ganglion (DRG) neuron and Schwann cell co-culture model to identify the signals associated with Rab27a during myelination. First, Slp2-a, as the Rab27a effector, was endogenously expressed in Schwann cells. Second, Rab27a expression significantly increased during Schwann cell myelination. Finally, Rab27a and Slp2-a silencing in Schwann cells not only reduced myelin protein expression, but also impaired formation of myelin-like membranes in DRG neuron and Schwann cell co-cultures. Our findings suggest that the Rab27a/Slp2-a complex affects Schwann cell myelination in vitro. Medknow Publications & Media Pvt Ltd 2016-11 /pmc/articles/PMC5204241/ /pubmed/28123429 http://dx.doi.org/10.4103/1673-5374.194755 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Research Article Su, Wen-feng Gu, Yun Wei, Zhong-ya Shen, Yun-tian Jin, Zi-han Yuan, Ying Gu, Xiao-song Chen, Gang Rab27a/Slp2-a complex is involved in Schwann cell myelination |
title | Rab27a/Slp2-a complex is involved in Schwann cell myelination |
title_full | Rab27a/Slp2-a complex is involved in Schwann cell myelination |
title_fullStr | Rab27a/Slp2-a complex is involved in Schwann cell myelination |
title_full_unstemmed | Rab27a/Slp2-a complex is involved in Schwann cell myelination |
title_short | Rab27a/Slp2-a complex is involved in Schwann cell myelination |
title_sort | rab27a/slp2-a complex is involved in schwann cell myelination |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5204241/ https://www.ncbi.nlm.nih.gov/pubmed/28123429 http://dx.doi.org/10.4103/1673-5374.194755 |
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