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Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis

Small RNAs are central players in RNA silencing, yet their cytoplasmic compartmentalization and the effects it may have on their activities have not been studied at the genomic scale. Here we report that Arabidopsis microRNAs (miRNAs) and small interfering RNAs (siRNAs) are distinctly partitioned be...

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Autores principales: Li, Shengben, Le, Brandon, Ma, Xuan, Li, Shaofang, You, Chenjiang, Yu, Yu, Zhang, Bailong, Liu, Lin, Gao, Lei, Shi, Ting, Zhao, Yonghui, Mo, Beixin, Cao, Xiaofeng, Chen, Xuemei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5207768/
https://www.ncbi.nlm.nih.gov/pubmed/27938667
http://dx.doi.org/10.7554/eLife.22750
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author Li, Shengben
Le, Brandon
Ma, Xuan
Li, Shaofang
You, Chenjiang
Yu, Yu
Zhang, Bailong
Liu, Lin
Gao, Lei
Shi, Ting
Zhao, Yonghui
Mo, Beixin
Cao, Xiaofeng
Chen, Xuemei
author_facet Li, Shengben
Le, Brandon
Ma, Xuan
Li, Shaofang
You, Chenjiang
Yu, Yu
Zhang, Bailong
Liu, Lin
Gao, Lei
Shi, Ting
Zhao, Yonghui
Mo, Beixin
Cao, Xiaofeng
Chen, Xuemei
author_sort Li, Shengben
collection PubMed
description Small RNAs are central players in RNA silencing, yet their cytoplasmic compartmentalization and the effects it may have on their activities have not been studied at the genomic scale. Here we report that Arabidopsis microRNAs (miRNAs) and small interfering RNAs (siRNAs) are distinctly partitioned between the endoplasmic reticulum (ER) and cytosol. All miRNAs are associated with membrane-bound polysomes (MBPs) as opposed to polysomes in general. The MBP association is functionally linked to a deeply conserved and tightly regulated activity of miRNAs – production of phased siRNAs (phasiRNAs) from select target RNAs. The phasiRNA precursor RNAs, thought to be noncoding, are on MBPs and are occupied by ribosomes in a manner that supports miRNA-triggered phasiRNA production, suggesting that ribosomes on the rough ER impact siRNA biogenesis. This study reveals global patterns of cytoplasmic partitioning of small RNAs and expands the known functions of ribosomes and ER. DOI: http://dx.doi.org/10.7554/eLife.22750.001
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spelling pubmed-52077682017-01-05 Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis Li, Shengben Le, Brandon Ma, Xuan Li, Shaofang You, Chenjiang Yu, Yu Zhang, Bailong Liu, Lin Gao, Lei Shi, Ting Zhao, Yonghui Mo, Beixin Cao, Xiaofeng Chen, Xuemei eLife Genes and Chromosomes Small RNAs are central players in RNA silencing, yet their cytoplasmic compartmentalization and the effects it may have on their activities have not been studied at the genomic scale. Here we report that Arabidopsis microRNAs (miRNAs) and small interfering RNAs (siRNAs) are distinctly partitioned between the endoplasmic reticulum (ER) and cytosol. All miRNAs are associated with membrane-bound polysomes (MBPs) as opposed to polysomes in general. The MBP association is functionally linked to a deeply conserved and tightly regulated activity of miRNAs – production of phased siRNAs (phasiRNAs) from select target RNAs. The phasiRNA precursor RNAs, thought to be noncoding, are on MBPs and are occupied by ribosomes in a manner that supports miRNA-triggered phasiRNA production, suggesting that ribosomes on the rough ER impact siRNA biogenesis. This study reveals global patterns of cytoplasmic partitioning of small RNAs and expands the known functions of ribosomes and ER. DOI: http://dx.doi.org/10.7554/eLife.22750.001 eLife Sciences Publications, Ltd 2016-12-12 /pmc/articles/PMC5207768/ /pubmed/27938667 http://dx.doi.org/10.7554/eLife.22750 Text en © 2016, Li et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Genes and Chromosomes
Li, Shengben
Le, Brandon
Ma, Xuan
Li, Shaofang
You, Chenjiang
Yu, Yu
Zhang, Bailong
Liu, Lin
Gao, Lei
Shi, Ting
Zhao, Yonghui
Mo, Beixin
Cao, Xiaofeng
Chen, Xuemei
Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis
title Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis
title_full Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis
title_fullStr Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis
title_full_unstemmed Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis
title_short Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis
title_sort biogenesis of phased sirnas on membrane-bound polysomes in arabidopsis
topic Genes and Chromosomes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5207768/
https://www.ncbi.nlm.nih.gov/pubmed/27938667
http://dx.doi.org/10.7554/eLife.22750
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