Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential

Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes we...

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Autores principales: Lisov, Alexander V., Belova, Oksana V., Lisova, Zoya A., Vinokurova, Nataliy G., Nagel, Alexey S., Andreeva-Kovalevskaya, Zhanna I., Budarina, Zhanna I., Nagornykh, Maxim O., Zakharova, Marina V., Shadrin, Andrey M., Solonin, Alexander S., Leontievsky, Alexey A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209306/
https://www.ncbi.nlm.nih.gov/pubmed/28050845
http://dx.doi.org/10.1186/s13568-016-0308-7
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author Lisov, Alexander V.
Belova, Oksana V.
Lisova, Zoya A.
Vinokurova, Nataliy G.
Nagel, Alexey S.
Andreeva-Kovalevskaya, Zhanna I.
Budarina, Zhanna I.
Nagornykh, Maxim O.
Zakharova, Marina V.
Shadrin, Andrey M.
Solonin, Alexander S.
Leontievsky, Alexey A.
author_facet Lisov, Alexander V.
Belova, Oksana V.
Lisova, Zoya A.
Vinokurova, Nataliy G.
Nagel, Alexey S.
Andreeva-Kovalevskaya, Zhanna I.
Budarina, Zhanna I.
Nagornykh, Maxim O.
Zakharova, Marina V.
Shadrin, Andrey M.
Solonin, Alexander S.
Leontievsky, Alexey A.
author_sort Lisov, Alexander V.
collection PubMed
description Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes were more stable at alkaline pH values. CFXyl1 and CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, and xylose, which is typical for GH10. CFXyl3 (GH11) and CFXyl4 (GH10) formed the same xylooligosaccharides, but xylose was formed in small amounts. The xylanases made efficient saccharification of rye, wheat and oat, common components of animal feed, which indicates their high biotechnological potential.
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spelling pubmed-52093062017-01-18 Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential Lisov, Alexander V. Belova, Oksana V. Lisova, Zoya A. Vinokurova, Nataliy G. Nagel, Alexey S. Andreeva-Kovalevskaya, Zhanna I. Budarina, Zhanna I. Nagornykh, Maxim O. Zakharova, Marina V. Shadrin, Andrey M. Solonin, Alexander S. Leontievsky, Alexey A. AMB Express Original Article Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes were more stable at alkaline pH values. CFXyl1 and CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, and xylose, which is typical for GH10. CFXyl3 (GH11) and CFXyl4 (GH10) formed the same xylooligosaccharides, but xylose was formed in small amounts. The xylanases made efficient saccharification of rye, wheat and oat, common components of animal feed, which indicates their high biotechnological potential. Springer Berlin Heidelberg 2017-01-03 /pmc/articles/PMC5209306/ /pubmed/28050845 http://dx.doi.org/10.1186/s13568-016-0308-7 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Lisov, Alexander V.
Belova, Oksana V.
Lisova, Zoya A.
Vinokurova, Nataliy G.
Nagel, Alexey S.
Andreeva-Kovalevskaya, Zhanna I.
Budarina, Zhanna I.
Nagornykh, Maxim O.
Zakharova, Marina V.
Shadrin, Andrey M.
Solonin, Alexander S.
Leontievsky, Alexey A.
Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential
title Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential
title_full Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential
title_fullStr Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential
title_full_unstemmed Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential
title_short Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential
title_sort xylanases of cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209306/
https://www.ncbi.nlm.nih.gov/pubmed/28050845
http://dx.doi.org/10.1186/s13568-016-0308-7
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