RNA-Guided Cas9-Induced Mutagenesis in Tobacco Followed by Efficient Genetic Fixation in Doubled Haploid Plants

Customizable endonucleases are providing an effective tool for genome engineering. The resulting primary transgenic individuals (T(0)) are typically heterozygous and/or chimeric with respect to any mutations induced. To generate genetically fixed mutants, they are conventionally allowed to self-poll...

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Detalles Bibliográficos
Autores principales: Schedel, Sindy, Pencs, Stefanie, Hensel, Götz, Müller, Andrea, Rutten, Twan, Kumlehn, Jochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209389/
https://www.ncbi.nlm.nih.gov/pubmed/28101094
http://dx.doi.org/10.3389/fpls.2016.01995

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209389/
https://www.ncbi.nlm.nih.gov/pubmed/28101094
http://dx.doi.org/10.3389/fpls.2016.01995

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