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RNA-Guided Cas9-Induced Mutagenesis in Tobacco Followed by Efficient Genetic Fixation in Doubled Haploid Plants
Customizable endonucleases are providing an effective tool for genome engineering. The resulting primary transgenic individuals (T(0)) are typically heterozygous and/or chimeric with respect to any mutations induced. To generate genetically fixed mutants, they are conventionally allowed to self-poll...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209389/ https://www.ncbi.nlm.nih.gov/pubmed/28101094 http://dx.doi.org/10.3389/fpls.2016.01995 |