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Solubilization of lipids and lipid phases by the styrene–maleic acid copolymer

A promising tool in membrane research is the use of the styrene–maleic acid (SMA) copolymer to solubilize membranes in the form of nanodiscs. Since membranes are heterogeneous in composition, it is important to know whether SMA thereby has a preference for solubilization of either specific types of...

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Autores principales: Dominguez Pardo, Juan J., Dörr, Jonas M., Iyer, Aditya, Cox, Ruud C., Scheidelaar, Stefan, Koorengevel, Martijn C., Subramaniam, Vinod, Killian, J. Antoinette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209432/
https://www.ncbi.nlm.nih.gov/pubmed/27815573
http://dx.doi.org/10.1007/s00249-016-1181-7
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author Dominguez Pardo, Juan J.
Dörr, Jonas M.
Iyer, Aditya
Cox, Ruud C.
Scheidelaar, Stefan
Koorengevel, Martijn C.
Subramaniam, Vinod
Killian, J. Antoinette
author_facet Dominguez Pardo, Juan J.
Dörr, Jonas M.
Iyer, Aditya
Cox, Ruud C.
Scheidelaar, Stefan
Koorengevel, Martijn C.
Subramaniam, Vinod
Killian, J. Antoinette
author_sort Dominguez Pardo, Juan J.
collection PubMed
description A promising tool in membrane research is the use of the styrene–maleic acid (SMA) copolymer to solubilize membranes in the form of nanodiscs. Since membranes are heterogeneous in composition, it is important to know whether SMA thereby has a preference for solubilization of either specific types of lipids or specific bilayer phases. Here, we investigated this by performing partial solubilization of model membranes and analyzing the lipid composition of the solubilized fraction. We found that SMA displays no significant lipid preference in homogeneous binary lipid mixtures in the fluid phase, even when using lipids that by themselves show very different solubilization kinetics. By contrast, in heterogeneous phase-separated bilayers, SMA was found to have a strong preference for solubilization of lipids in the fluid phase as compared to those in either a gel phase or a liquid-ordered phase. Together the results suggest that (1) SMA is a reliable tool to characterize native interactions between membrane constituents, (2) any solubilization preference of SMA is not due to properties of individual lipids but rather due to properties of the membrane or membrane domains in which these lipids reside and (3) exploiting SMA resistance rather than detergent resistance may be an attractive approach for the isolation of ordered domains from biological membranes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00249-016-1181-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-52094322017-01-18 Solubilization of lipids and lipid phases by the styrene–maleic acid copolymer Dominguez Pardo, Juan J. Dörr, Jonas M. Iyer, Aditya Cox, Ruud C. Scheidelaar, Stefan Koorengevel, Martijn C. Subramaniam, Vinod Killian, J. Antoinette Eur Biophys J Biophysics Letter A promising tool in membrane research is the use of the styrene–maleic acid (SMA) copolymer to solubilize membranes in the form of nanodiscs. Since membranes are heterogeneous in composition, it is important to know whether SMA thereby has a preference for solubilization of either specific types of lipids or specific bilayer phases. Here, we investigated this by performing partial solubilization of model membranes and analyzing the lipid composition of the solubilized fraction. We found that SMA displays no significant lipid preference in homogeneous binary lipid mixtures in the fluid phase, even when using lipids that by themselves show very different solubilization kinetics. By contrast, in heterogeneous phase-separated bilayers, SMA was found to have a strong preference for solubilization of lipids in the fluid phase as compared to those in either a gel phase or a liquid-ordered phase. Together the results suggest that (1) SMA is a reliable tool to characterize native interactions between membrane constituents, (2) any solubilization preference of SMA is not due to properties of individual lipids but rather due to properties of the membrane or membrane domains in which these lipids reside and (3) exploiting SMA resistance rather than detergent resistance may be an attractive approach for the isolation of ordered domains from biological membranes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00249-016-1181-7) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-11-04 2017 /pmc/articles/PMC5209432/ /pubmed/27815573 http://dx.doi.org/10.1007/s00249-016-1181-7 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Biophysics Letter
Dominguez Pardo, Juan J.
Dörr, Jonas M.
Iyer, Aditya
Cox, Ruud C.
Scheidelaar, Stefan
Koorengevel, Martijn C.
Subramaniam, Vinod
Killian, J. Antoinette
Solubilization of lipids and lipid phases by the styrene–maleic acid copolymer
title Solubilization of lipids and lipid phases by the styrene–maleic acid copolymer
title_full Solubilization of lipids and lipid phases by the styrene–maleic acid copolymer
title_fullStr Solubilization of lipids and lipid phases by the styrene–maleic acid copolymer
title_full_unstemmed Solubilization of lipids and lipid phases by the styrene–maleic acid copolymer
title_short Solubilization of lipids and lipid phases by the styrene–maleic acid copolymer
title_sort solubilization of lipids and lipid phases by the styrene–maleic acid copolymer
topic Biophysics Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209432/
https://www.ncbi.nlm.nih.gov/pubmed/27815573
http://dx.doi.org/10.1007/s00249-016-1181-7
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