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Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection
Human papillomavirus (HPV) infection is an important etiologic factor in cervical carcinogenesis. Various HPV DNA detection methods have been evaluated for clinicopathological level. For the specimens with normal cytological finding, discrepancies among the detection methods were frequently found an...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209590/ https://www.ncbi.nlm.nih.gov/pubmed/28097135 http://dx.doi.org/10.1155/2016/5170419 |
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author | Kim, Taek Soo Lim, Mi Suk Hong, Yun Ji Hwang, Sang Mee Park, Kyoung Un Song, Junghan Kim, Eui-Chong |
author_facet | Kim, Taek Soo Lim, Mi Suk Hong, Yun Ji Hwang, Sang Mee Park, Kyoung Un Song, Junghan Kim, Eui-Chong |
author_sort | Kim, Taek Soo |
collection | PubMed |
description | Human papillomavirus (HPV) infection is an important etiologic factor in cervical carcinogenesis. Various HPV DNA detection methods have been evaluated for clinicopathological level. For the specimens with normal cytological finding, discrepancies among the detection methods were frequently found and adequate interpretation can be difficult. 6,322 clinical specimens were submitted and evaluated for real-time PCR and Hybrid Capture 2 (HC2). 573 positive or “Not Detected but Amplified” (NDBA) specimens by real-time PCR were additionally tested using genetic analyzer. For the reliability of real-time PCR, 325 retests were performed. Optimal cut-off cycle threshold (C (T)) value was evaluated also. 78.7% of submitted specimens showed normal or nonspecific cytological finding. The distributions of HPV types by real-time PCR were not different between positive and NDBA cases. For positive cases by fragment analysis, concordance rates with real-time PCR and HC2 were 94.2% and 84.2%. In NDBA cases, fragment analysis and real-time PCR showed identical results in 77.0% and HC2 revealed 27.6% of concordance with fragment analysis. Optimal cut-off C (T) value was different for HPV types. NDBA results in real-time PCR should be regarded as equivocal, not negative. The adjustment of cut-off C (T) value for HPV types will be helpful for the appropriate result interpretation. |
format | Online Article Text |
id | pubmed-5209590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-52095902017-01-17 Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection Kim, Taek Soo Lim, Mi Suk Hong, Yun Ji Hwang, Sang Mee Park, Kyoung Un Song, Junghan Kim, Eui-Chong Biomed Res Int Research Article Human papillomavirus (HPV) infection is an important etiologic factor in cervical carcinogenesis. Various HPV DNA detection methods have been evaluated for clinicopathological level. For the specimens with normal cytological finding, discrepancies among the detection methods were frequently found and adequate interpretation can be difficult. 6,322 clinical specimens were submitted and evaluated for real-time PCR and Hybrid Capture 2 (HC2). 573 positive or “Not Detected but Amplified” (NDBA) specimens by real-time PCR were additionally tested using genetic analyzer. For the reliability of real-time PCR, 325 retests were performed. Optimal cut-off cycle threshold (C (T)) value was evaluated also. 78.7% of submitted specimens showed normal or nonspecific cytological finding. The distributions of HPV types by real-time PCR were not different between positive and NDBA cases. For positive cases by fragment analysis, concordance rates with real-time PCR and HC2 were 94.2% and 84.2%. In NDBA cases, fragment analysis and real-time PCR showed identical results in 77.0% and HC2 revealed 27.6% of concordance with fragment analysis. Optimal cut-off C (T) value was different for HPV types. NDBA results in real-time PCR should be regarded as equivocal, not negative. The adjustment of cut-off C (T) value for HPV types will be helpful for the appropriate result interpretation. Hindawi Publishing Corporation 2016 2016-12-21 /pmc/articles/PMC5209590/ /pubmed/28097135 http://dx.doi.org/10.1155/2016/5170419 Text en |
spellingShingle | Research Article Kim, Taek Soo Lim, Mi Suk Hong, Yun Ji Hwang, Sang Mee Park, Kyoung Un Song, Junghan Kim, Eui-Chong Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection |
title | Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection |
title_full | Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection |
title_fullStr | Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection |
title_full_unstemmed | Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection |
title_short | Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection |
title_sort | significance of “not detected but amplified” results by real-time pcr method for hpv dna detection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209590/ https://www.ncbi.nlm.nih.gov/pubmed/28097135 http://dx.doi.org/10.1155/2016/5170419 |
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