Cargando…

Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury

Fermented Chinese formula Shuan-Tong-Ling (STL), composed of fourteen medicinal herbs, was an experiential formula by Dr. Zhigang Mei for treating vascular encephalopathy, but the underlying mechanisms remained unknown. In this study, we aimed to investigate the protective effects of fermented STL o...

Descripción completa

Detalles Bibliográficos
Autores principales: Tan, Lingjing, Zhang, Xiang, Mei, Zhigang, Wang, Jinfeng, Li, Xiaoli, Huang, Weifeng, Yang, Songbai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209619/
https://www.ncbi.nlm.nih.gov/pubmed/28096886
http://dx.doi.org/10.1155/2016/5154290
_version_ 1782490762263396352
author Tan, Lingjing
Zhang, Xiang
Mei, Zhigang
Wang, Jinfeng
Li, Xiaoli
Huang, Weifeng
Yang, Songbai
author_facet Tan, Lingjing
Zhang, Xiang
Mei, Zhigang
Wang, Jinfeng
Li, Xiaoli
Huang, Weifeng
Yang, Songbai
author_sort Tan, Lingjing
collection PubMed
description Fermented Chinese formula Shuan-Tong-Ling (STL), composed of fourteen medicinal herbs, was an experiential formula by Dr. Zhigang Mei for treating vascular encephalopathy, but the underlying mechanisms remained unknown. In this study, we aimed to investigate the protective effects of fermented STL on hydrogen peroxide- (H(2)O(2)-) induced injury in rat brain microvascular endothelial cells (BMECs) and the possible mechanisms. Cultured BMECs were treated with H(2)O(2), STL, or nicotinamide (NAM, a SIRT1 inhibitor). Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was employed to detect cell proliferation and senescence-associated beta-galactosidase (SA-β-gal) was used to examine cell senescence. Cell nuclei were observed by 4′,6-diamidino-2-phenylindole. Additionally, changes in reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione (GSH) levels were measured. Expression of SIRT1, p21, and PGC-1α was determined by western blot. Cell proliferation significantly increased with STL treatment in a dose-dependent manner. H(2)O(2) treatment could intensify cell senescence and nuclei splitting or pyknosis. With STL treatment, the reduced ROS level was accompanied by increased SOD and GSH activity. Further assays showed upregulation of SIRT1 and PGC-1α and downregulation of p21 after STL treatment. The results revealed that STL could protect BMECs against oxidative stress injury at least partially through the SIRT1 pathway.
format Online
Article
Text
id pubmed-5209619
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-52096192017-01-17 Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury Tan, Lingjing Zhang, Xiang Mei, Zhigang Wang, Jinfeng Li, Xiaoli Huang, Weifeng Yang, Songbai Evid Based Complement Alternat Med Research Article Fermented Chinese formula Shuan-Tong-Ling (STL), composed of fourteen medicinal herbs, was an experiential formula by Dr. Zhigang Mei for treating vascular encephalopathy, but the underlying mechanisms remained unknown. In this study, we aimed to investigate the protective effects of fermented STL on hydrogen peroxide- (H(2)O(2)-) induced injury in rat brain microvascular endothelial cells (BMECs) and the possible mechanisms. Cultured BMECs were treated with H(2)O(2), STL, or nicotinamide (NAM, a SIRT1 inhibitor). Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was employed to detect cell proliferation and senescence-associated beta-galactosidase (SA-β-gal) was used to examine cell senescence. Cell nuclei were observed by 4′,6-diamidino-2-phenylindole. Additionally, changes in reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione (GSH) levels were measured. Expression of SIRT1, p21, and PGC-1α was determined by western blot. Cell proliferation significantly increased with STL treatment in a dose-dependent manner. H(2)O(2) treatment could intensify cell senescence and nuclei splitting or pyknosis. With STL treatment, the reduced ROS level was accompanied by increased SOD and GSH activity. Further assays showed upregulation of SIRT1 and PGC-1α and downregulation of p21 after STL treatment. The results revealed that STL could protect BMECs against oxidative stress injury at least partially through the SIRT1 pathway. Hindawi Publishing Corporation 2016 2016-12-21 /pmc/articles/PMC5209619/ /pubmed/28096886 http://dx.doi.org/10.1155/2016/5154290 Text en Copyright © 2016 Lingjing Tan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tan, Lingjing
Zhang, Xiang
Mei, Zhigang
Wang, Jinfeng
Li, Xiaoli
Huang, Weifeng
Yang, Songbai
Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury
title Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury
title_full Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury
title_fullStr Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury
title_full_unstemmed Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury
title_short Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury
title_sort fermented chinese formula shuan-tong-ling protects brain microvascular endothelial cells against oxidative stress injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209619/
https://www.ncbi.nlm.nih.gov/pubmed/28096886
http://dx.doi.org/10.1155/2016/5154290
work_keys_str_mv AT tanlingjing fermentedchineseformulashuantonglingprotectsbrainmicrovascularendothelialcellsagainstoxidativestressinjury
AT zhangxiang fermentedchineseformulashuantonglingprotectsbrainmicrovascularendothelialcellsagainstoxidativestressinjury
AT meizhigang fermentedchineseformulashuantonglingprotectsbrainmicrovascularendothelialcellsagainstoxidativestressinjury
AT wangjinfeng fermentedchineseformulashuantonglingprotectsbrainmicrovascularendothelialcellsagainstoxidativestressinjury
AT lixiaoli fermentedchineseformulashuantonglingprotectsbrainmicrovascularendothelialcellsagainstoxidativestressinjury
AT huangweifeng fermentedchineseformulashuantonglingprotectsbrainmicrovascularendothelialcellsagainstoxidativestressinjury
AT yangsongbai fermentedchineseformulashuantonglingprotectsbrainmicrovascularendothelialcellsagainstoxidativestressinjury