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U24 from Roseolovirus interacts strongly with Nedd4 WW Domains

U24 is a protein found in both roseoloviruses Human Herpes Virus type 6 and 7 (HHV-6 and HHV-7), with an N-terminus that is rich in prolines (PY motif in both HHV-6A and 7; PxxP motif in HHV-6A). Previous work has shown that the interaction between U24 and WW domains is important for endocytic recyc...

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Autores principales: Sang, Yurou, Zhang, Rui, Scott, Walter R. P., Creagh, A. Louise, Haynes, Charles A., Straus, Suzana K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209733/
https://www.ncbi.nlm.nih.gov/pubmed/28051106
http://dx.doi.org/10.1038/srep39776
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author Sang, Yurou
Zhang, Rui
Scott, Walter R. P.
Creagh, A. Louise
Haynes, Charles A.
Straus, Suzana K.
author_facet Sang, Yurou
Zhang, Rui
Scott, Walter R. P.
Creagh, A. Louise
Haynes, Charles A.
Straus, Suzana K.
author_sort Sang, Yurou
collection PubMed
description U24 is a protein found in both roseoloviruses Human Herpes Virus type 6 and 7 (HHV-6 and HHV-7), with an N-terminus that is rich in prolines (PY motif in both HHV-6A and 7; PxxP motif in HHV-6A). Previous work has shown that the interaction between U24 and WW domains is important for endocytic recycling of T-cell receptors, but a cognate ligand was never identified. In this contribution, data was obtained from pull-downs, ITC, NMR and molecular dynamics simulations to show that a specific interaction exists between U24 and Nedd4 WW domains. ITC experiments were also carried out for U24 from HHV-6A phosphorylated at Thr6 (pU24-6A) and a peptide containing the PY motif from Nogo-A, a protein implicated in both the initial inflammatory and the neurodegenerative phases of multiple sclerosis (MS). The results suggest that phosphorylation of U24 from HHV-6A may be crucial for its potential role in MS.
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spelling pubmed-52097332017-01-05 U24 from Roseolovirus interacts strongly with Nedd4 WW Domains Sang, Yurou Zhang, Rui Scott, Walter R. P. Creagh, A. Louise Haynes, Charles A. Straus, Suzana K. Sci Rep Article U24 is a protein found in both roseoloviruses Human Herpes Virus type 6 and 7 (HHV-6 and HHV-7), with an N-terminus that is rich in prolines (PY motif in both HHV-6A and 7; PxxP motif in HHV-6A). Previous work has shown that the interaction between U24 and WW domains is important for endocytic recycling of T-cell receptors, but a cognate ligand was never identified. In this contribution, data was obtained from pull-downs, ITC, NMR and molecular dynamics simulations to show that a specific interaction exists between U24 and Nedd4 WW domains. ITC experiments were also carried out for U24 from HHV-6A phosphorylated at Thr6 (pU24-6A) and a peptide containing the PY motif from Nogo-A, a protein implicated in both the initial inflammatory and the neurodegenerative phases of multiple sclerosis (MS). The results suggest that phosphorylation of U24 from HHV-6A may be crucial for its potential role in MS. Nature Publishing Group 2017-01-04 /pmc/articles/PMC5209733/ /pubmed/28051106 http://dx.doi.org/10.1038/srep39776 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sang, Yurou
Zhang, Rui
Scott, Walter R. P.
Creagh, A. Louise
Haynes, Charles A.
Straus, Suzana K.
U24 from Roseolovirus interacts strongly with Nedd4 WW Domains
title U24 from Roseolovirus interacts strongly with Nedd4 WW Domains
title_full U24 from Roseolovirus interacts strongly with Nedd4 WW Domains
title_fullStr U24 from Roseolovirus interacts strongly with Nedd4 WW Domains
title_full_unstemmed U24 from Roseolovirus interacts strongly with Nedd4 WW Domains
title_short U24 from Roseolovirus interacts strongly with Nedd4 WW Domains
title_sort u24 from roseolovirus interacts strongly with nedd4 ww domains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209733/
https://www.ncbi.nlm.nih.gov/pubmed/28051106
http://dx.doi.org/10.1038/srep39776
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