Cargando…

Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations

BACKGROUND: Small RNAs present in bovine ejaculate can be linked to sperm abnormalities and fertility disorders. At present, quality parameters routinely used in semen evaluation are not fully reliable to predict bull fertility. In order to provide additional quality measurements for cryopreserved s...

Descripción completa

Detalles Bibliográficos
Autores principales: Capra, E., Turri, F., Lazzari, B., Cremonesi, P., Gliozzi, T. M., Fojadelli, I., Stella, A., Pizzi, F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209821/
https://www.ncbi.nlm.nih.gov/pubmed/28052756
http://dx.doi.org/10.1186/s12864-016-3394-7
_version_ 1782490799478407168
author Capra, E.
Turri, F.
Lazzari, B.
Cremonesi, P.
Gliozzi, T. M.
Fojadelli, I.
Stella, A.
Pizzi, F.
author_facet Capra, E.
Turri, F.
Lazzari, B.
Cremonesi, P.
Gliozzi, T. M.
Fojadelli, I.
Stella, A.
Pizzi, F.
author_sort Capra, E.
collection PubMed
description BACKGROUND: Small RNAs present in bovine ejaculate can be linked to sperm abnormalities and fertility disorders. At present, quality parameters routinely used in semen evaluation are not fully reliable to predict bull fertility. In order to provide additional quality measurements for cryopreserved semen used for breeding, a method based on deep sequencing of sperm microRNA (miRNA) and Piwi-interacting RNA (piRNA) from individual bulls was developed. To validate our method, two populations of spermatozoa isolated from high and low motile fractions separated by Percoll were sequenced, and their small RNAs content characterized. RESULTS: Sperm cells from frozen thawed semen samples of 4 bulls were successfully separated in two fractions. We identified 83 miRNAs and 79 putative piRNAs clusters that were differentially expressed in both fractions. Gene pathways targeted by 40 known differentially expressed miRNAs were related to apoptosis. Dysregulation of miR-17-5p, miR-26a-5p, miR-486-5p, miR-122-5p, miR-184 and miR-20a-5p was found to target three pathways (PTEN, PI3K/AKT and STAT). CONCLUSIONS: Small RNAs sequencing data obtained from single bulls are consistent with previous findings. Specific miRNAs are differentially represented in low versus high motile sperm, suggesting an alteration of cell functions and increased germ cell apoptosis in the low motile fraction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-3394-7) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5209821
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-52098212017-01-04 Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations Capra, E. Turri, F. Lazzari, B. Cremonesi, P. Gliozzi, T. M. Fojadelli, I. Stella, A. Pizzi, F. BMC Genomics Research Article BACKGROUND: Small RNAs present in bovine ejaculate can be linked to sperm abnormalities and fertility disorders. At present, quality parameters routinely used in semen evaluation are not fully reliable to predict bull fertility. In order to provide additional quality measurements for cryopreserved semen used for breeding, a method based on deep sequencing of sperm microRNA (miRNA) and Piwi-interacting RNA (piRNA) from individual bulls was developed. To validate our method, two populations of spermatozoa isolated from high and low motile fractions separated by Percoll were sequenced, and their small RNAs content characterized. RESULTS: Sperm cells from frozen thawed semen samples of 4 bulls were successfully separated in two fractions. We identified 83 miRNAs and 79 putative piRNAs clusters that were differentially expressed in both fractions. Gene pathways targeted by 40 known differentially expressed miRNAs were related to apoptosis. Dysregulation of miR-17-5p, miR-26a-5p, miR-486-5p, miR-122-5p, miR-184 and miR-20a-5p was found to target three pathways (PTEN, PI3K/AKT and STAT). CONCLUSIONS: Small RNAs sequencing data obtained from single bulls are consistent with previous findings. Specific miRNAs are differentially represented in low versus high motile sperm, suggesting an alteration of cell functions and increased germ cell apoptosis in the low motile fraction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-3394-7) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-04 /pmc/articles/PMC5209821/ /pubmed/28052756 http://dx.doi.org/10.1186/s12864-016-3394-7 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Capra, E.
Turri, F.
Lazzari, B.
Cremonesi, P.
Gliozzi, T. M.
Fojadelli, I.
Stella, A.
Pizzi, F.
Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations
title Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations
title_full Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations
title_fullStr Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations
title_full_unstemmed Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations
title_short Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations
title_sort small rna sequencing of cryopreserved semen from single bull revealed altered mirnas and pirnas expression between high- and low-motile sperm populations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209821/
https://www.ncbi.nlm.nih.gov/pubmed/28052756
http://dx.doi.org/10.1186/s12864-016-3394-7
work_keys_str_mv AT caprae smallrnasequencingofcryopreservedsemenfromsinglebullrevealedalteredmirnasandpirnasexpressionbetweenhighandlowmotilespermpopulations
AT turrif smallrnasequencingofcryopreservedsemenfromsinglebullrevealedalteredmirnasandpirnasexpressionbetweenhighandlowmotilespermpopulations
AT lazzarib smallrnasequencingofcryopreservedsemenfromsinglebullrevealedalteredmirnasandpirnasexpressionbetweenhighandlowmotilespermpopulations
AT cremonesip smallrnasequencingofcryopreservedsemenfromsinglebullrevealedalteredmirnasandpirnasexpressionbetweenhighandlowmotilespermpopulations
AT gliozzitm smallrnasequencingofcryopreservedsemenfromsinglebullrevealedalteredmirnasandpirnasexpressionbetweenhighandlowmotilespermpopulations
AT fojadellii smallrnasequencingofcryopreservedsemenfromsinglebullrevealedalteredmirnasandpirnasexpressionbetweenhighandlowmotilespermpopulations
AT stellaa smallrnasequencingofcryopreservedsemenfromsinglebullrevealedalteredmirnasandpirnasexpressionbetweenhighandlowmotilespermpopulations
AT pizzif smallrnasequencingofcryopreservedsemenfromsinglebullrevealedalteredmirnasandpirnasexpressionbetweenhighandlowmotilespermpopulations