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Mouse mammary tumor-like virus (MMTV) is present in human breast tissue before development of virally associated breast cancer

BACKGROUND: There is substantial evidence that a virus homologous to mouse mammary tumor virus (MMTV) may have a role in human breast cancer. The present study indicates that those who developed breast cancer associated with an MMTV-like virus had this virus in their non-cancerous breast tissues yea...

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Autores principales: Nartey, Teiko, Mazzanti, Chiara M., Melana, Stella, Glenn, Wendy K., Bevilacqua, Generoso, Holland, James F., Whitaker, Noel J., Lawson, James S., Pogo, Beatriz G.T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209856/
https://www.ncbi.nlm.nih.gov/pubmed/28053656
http://dx.doi.org/10.1186/s13027-016-0113-6
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author Nartey, Teiko
Mazzanti, Chiara M.
Melana, Stella
Glenn, Wendy K.
Bevilacqua, Generoso
Holland, James F.
Whitaker, Noel J.
Lawson, James S.
Pogo, Beatriz G.T.
author_facet Nartey, Teiko
Mazzanti, Chiara M.
Melana, Stella
Glenn, Wendy K.
Bevilacqua, Generoso
Holland, James F.
Whitaker, Noel J.
Lawson, James S.
Pogo, Beatriz G.T.
author_sort Nartey, Teiko
collection PubMed
description BACKGROUND: There is substantial evidence that a virus homologous to mouse mammary tumor virus (MMTV) may have a role in human breast cancer. The present study indicates that those who developed breast cancer associated with an MMTV-like virus had this virus in their non-cancerous breast tissues years before the cancer developed. METHODS: Polymerase chain reaction (PCR) techniques and sequencing were used to identify MMTV-like envelope gene sequences (MMTV-like env sequences) in Australian benign breast biopsy specimens from women who several years later developed breast cancer. Murine contamination was excluded by stringent laboratory procedures, and the absence of intracisternal A particle sequences and mitochondrial cyclooxygenase sequences. RESULTS: MMTV-like env sequences (also called HMTV sequences to denote their source) were found in 9 of 25 breast cancer specimens (36%). Among 25 non-cancerous breast biopsies of these same patients taken 1 to 11 years earlier, six contained MMTV-like sequences (24%). Five of the six were among the nine virally-associated breast cancers. In two pairs of specimens, benign and malignant, env sequences were 97% identical. CONCLUSIONS: The identification of MMTV (MMTV-like) sequences in breast tissues prior to the development of MMTV positive breast cancer fulfills a key criterion for a possible causal role for the MMTV-like virus in human breast cancer.
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spelling pubmed-52098562017-01-04 Mouse mammary tumor-like virus (MMTV) is present in human breast tissue before development of virally associated breast cancer Nartey, Teiko Mazzanti, Chiara M. Melana, Stella Glenn, Wendy K. Bevilacqua, Generoso Holland, James F. Whitaker, Noel J. Lawson, James S. Pogo, Beatriz G.T. Infect Agent Cancer Research Article BACKGROUND: There is substantial evidence that a virus homologous to mouse mammary tumor virus (MMTV) may have a role in human breast cancer. The present study indicates that those who developed breast cancer associated with an MMTV-like virus had this virus in their non-cancerous breast tissues years before the cancer developed. METHODS: Polymerase chain reaction (PCR) techniques and sequencing were used to identify MMTV-like envelope gene sequences (MMTV-like env sequences) in Australian benign breast biopsy specimens from women who several years later developed breast cancer. Murine contamination was excluded by stringent laboratory procedures, and the absence of intracisternal A particle sequences and mitochondrial cyclooxygenase sequences. RESULTS: MMTV-like env sequences (also called HMTV sequences to denote their source) were found in 9 of 25 breast cancer specimens (36%). Among 25 non-cancerous breast biopsies of these same patients taken 1 to 11 years earlier, six contained MMTV-like sequences (24%). Five of the six were among the nine virally-associated breast cancers. In two pairs of specimens, benign and malignant, env sequences were 97% identical. CONCLUSIONS: The identification of MMTV (MMTV-like) sequences in breast tissues prior to the development of MMTV positive breast cancer fulfills a key criterion for a possible causal role for the MMTV-like virus in human breast cancer. BioMed Central 2017-01-04 /pmc/articles/PMC5209856/ /pubmed/28053656 http://dx.doi.org/10.1186/s13027-016-0113-6 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Nartey, Teiko
Mazzanti, Chiara M.
Melana, Stella
Glenn, Wendy K.
Bevilacqua, Generoso
Holland, James F.
Whitaker, Noel J.
Lawson, James S.
Pogo, Beatriz G.T.
Mouse mammary tumor-like virus (MMTV) is present in human breast tissue before development of virally associated breast cancer
title Mouse mammary tumor-like virus (MMTV) is present in human breast tissue before development of virally associated breast cancer
title_full Mouse mammary tumor-like virus (MMTV) is present in human breast tissue before development of virally associated breast cancer
title_fullStr Mouse mammary tumor-like virus (MMTV) is present in human breast tissue before development of virally associated breast cancer
title_full_unstemmed Mouse mammary tumor-like virus (MMTV) is present in human breast tissue before development of virally associated breast cancer
title_short Mouse mammary tumor-like virus (MMTV) is present in human breast tissue before development of virally associated breast cancer
title_sort mouse mammary tumor-like virus (mmtv) is present in human breast tissue before development of virally associated breast cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209856/
https://www.ncbi.nlm.nih.gov/pubmed/28053656
http://dx.doi.org/10.1186/s13027-016-0113-6
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