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Free radical scavenging decreases endothelin‐1 excretion and glomerular albumin permeability during type 1 diabetes

Increased renal endothelin‐1 (ET‐1) production and an ET(A) receptor‐dependent increase in glomerular albumin permeability (P(alb)) accompany type 1 diabetes mellitus (T1D). We hypothesized that T1D‐induced oxidative stress contributes to renal ET‐1 production and glomerular P(alb). Male rats with s...

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Autores principales: Saleh, Mohamed A., De Miguel, Carmen, Stevens, David I., Carmines, Pamela K., Pollock, David M., Pollock, Jennifer S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5210388/
https://www.ncbi.nlm.nih.gov/pubmed/28039404
http://dx.doi.org/10.14814/phy2.13055
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author Saleh, Mohamed A.
De Miguel, Carmen
Stevens, David I.
Carmines, Pamela K.
Pollock, David M.
Pollock, Jennifer S.
author_facet Saleh, Mohamed A.
De Miguel, Carmen
Stevens, David I.
Carmines, Pamela K.
Pollock, David M.
Pollock, Jennifer S.
author_sort Saleh, Mohamed A.
collection PubMed
description Increased renal endothelin‐1 (ET‐1) production and an ET(A) receptor‐dependent increase in glomerular albumin permeability (P(alb)) accompany type 1 diabetes mellitus (T1D). We hypothesized that T1D‐induced oxidative stress contributes to renal ET‐1 production and glomerular P(alb). Male rats with streptozotocin‐induced T1D were provided free access to drinking water without additives (T1D rats) or containing the free radical scavenger tempol (1 mmol/L; T1D+Tempol). After 3 weeks, T1D+Tempol rats displayed lower urinary excretion of thiobarbituric acid reactive substances and glomerular superoxide production (dihydroethidium staining) compared to T1D rats. Urinary ET‐1 excretion and inner medullary (but not cortical or outer medullary) prepro‐ET‐1 mRNA expression were lower in the T1D+Tempol group than in the T1D group. P(alb), measured as the change in volume of isolated glomeruli upon exposure to oncotic gradients of albumin, was significantly lower in the T1D+Tempol group than in the T1D group. Tempol treatment did not alter protein excretion or creatinine clearance. These data support the postulate that oxidative stress contributes to glomerular P(alb) and renal ET‐1 production during the early phase of type 1 diabetes.
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spelling pubmed-52103882017-01-05 Free radical scavenging decreases endothelin‐1 excretion and glomerular albumin permeability during type 1 diabetes Saleh, Mohamed A. De Miguel, Carmen Stevens, David I. Carmines, Pamela K. Pollock, David M. Pollock, Jennifer S. Physiol Rep Original Research Increased renal endothelin‐1 (ET‐1) production and an ET(A) receptor‐dependent increase in glomerular albumin permeability (P(alb)) accompany type 1 diabetes mellitus (T1D). We hypothesized that T1D‐induced oxidative stress contributes to renal ET‐1 production and glomerular P(alb). Male rats with streptozotocin‐induced T1D were provided free access to drinking water without additives (T1D rats) or containing the free radical scavenger tempol (1 mmol/L; T1D+Tempol). After 3 weeks, T1D+Tempol rats displayed lower urinary excretion of thiobarbituric acid reactive substances and glomerular superoxide production (dihydroethidium staining) compared to T1D rats. Urinary ET‐1 excretion and inner medullary (but not cortical or outer medullary) prepro‐ET‐1 mRNA expression were lower in the T1D+Tempol group than in the T1D group. P(alb), measured as the change in volume of isolated glomeruli upon exposure to oncotic gradients of albumin, was significantly lower in the T1D+Tempol group than in the T1D group. Tempol treatment did not alter protein excretion or creatinine clearance. These data support the postulate that oxidative stress contributes to glomerular P(alb) and renal ET‐1 production during the early phase of type 1 diabetes. John Wiley and Sons Inc. 2016-12-30 /pmc/articles/PMC5210388/ /pubmed/28039404 http://dx.doi.org/10.14814/phy2.13055 Text en © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Saleh, Mohamed A.
De Miguel, Carmen
Stevens, David I.
Carmines, Pamela K.
Pollock, David M.
Pollock, Jennifer S.
Free radical scavenging decreases endothelin‐1 excretion and glomerular albumin permeability during type 1 diabetes
title Free radical scavenging decreases endothelin‐1 excretion and glomerular albumin permeability during type 1 diabetes
title_full Free radical scavenging decreases endothelin‐1 excretion and glomerular albumin permeability during type 1 diabetes
title_fullStr Free radical scavenging decreases endothelin‐1 excretion and glomerular albumin permeability during type 1 diabetes
title_full_unstemmed Free radical scavenging decreases endothelin‐1 excretion and glomerular albumin permeability during type 1 diabetes
title_short Free radical scavenging decreases endothelin‐1 excretion and glomerular albumin permeability during type 1 diabetes
title_sort free radical scavenging decreases endothelin‐1 excretion and glomerular albumin permeability during type 1 diabetes
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5210388/
https://www.ncbi.nlm.nih.gov/pubmed/28039404
http://dx.doi.org/10.14814/phy2.13055
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