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Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry

The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to enter the market. As the development of new antibiotics is lengthy and costly, early monitoring of compound's toxicity is essential in the development of novel agents. Our...

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Detalles Bibliográficos
Autores principales: Pick, Neora, Cameron, Scott, Arad, Dorit, Av-Gay, Yossef
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521344/
https://www.ncbi.nlm.nih.gov/pubmed/15472722
http://dx.doi.org/10.1251/bpo92
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author Pick, Neora
Cameron, Scott
Arad, Dorit
Av-Gay, Yossef
author_facet Pick, Neora
Cameron, Scott
Arad, Dorit
Av-Gay, Yossef
author_sort Pick, Neora
collection PubMed
description The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to enter the market. As the development of new antibiotics is lengthy and costly, early monitoring of compound's toxicity is essential in the development of novel agents. Our interest is in a rapid, simple, high throughput screening method to assess cytotoxicity induced by potential agents. Some intracellular pathogens, such as Mycobacterium tuberculosis primary site of infection is human alveolar macrophages. Thus, evaluation of candidate drugs for macrophage toxicity is crucial. Protocols for high throughput drug toxicity screening of macrophages using flow cytometry are lacking in the literature. For this application we modified a preexisting technique, propidium iodide (PI) exclusion staining and utilized it for rapid toxicity tests. Samples were prepared in 96 well plates and analyzed by flow cytometry, which allowed for rapid, inexpensive and precise assessment of compound's toxicity associated with cell death.
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spelling pubmed-5213442004-10-06 Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry Pick, Neora Cameron, Scott Arad, Dorit Av-Gay, Yossef Biol Proced Online Research Article The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to enter the market. As the development of new antibiotics is lengthy and costly, early monitoring of compound's toxicity is essential in the development of novel agents. Our interest is in a rapid, simple, high throughput screening method to assess cytotoxicity induced by potential agents. Some intracellular pathogens, such as Mycobacterium tuberculosis primary site of infection is human alveolar macrophages. Thus, evaluation of candidate drugs for macrophage toxicity is crucial. Protocols for high throughput drug toxicity screening of macrophages using flow cytometry are lacking in the literature. For this application we modified a preexisting technique, propidium iodide (PI) exclusion staining and utilized it for rapid toxicity tests. Samples were prepared in 96 well plates and analyzed by flow cytometry, which allowed for rapid, inexpensive and precise assessment of compound's toxicity associated with cell death. Biological Procedures Online 2004-10-01 /pmc/articles/PMC521344/ /pubmed/15472722 http://dx.doi.org/10.1251/bpo92 Text en Copyright © October 10, 2004, N Pick et al. This paper is Open Access and is published in Biological Procedures Online under license from the authors. Copying, printing, redistribution and storage permitted.
spellingShingle Research Article
Pick, Neora
Cameron, Scott
Arad, Dorit
Av-Gay, Yossef
Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry
title Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry
title_full Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry
title_fullStr Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry
title_full_unstemmed Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry
title_short Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry
title_sort screening of compounds toxicity against human monocytic cell line-thp-1 by flow cytometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521344/
https://www.ncbi.nlm.nih.gov/pubmed/15472722
http://dx.doi.org/10.1251/bpo92
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