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Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry
The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to enter the market. As the development of new antibiotics is lengthy and costly, early monitoring of compound's toxicity is essential in the development of novel agents. Our...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Biological Procedures Online
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521344/ https://www.ncbi.nlm.nih.gov/pubmed/15472722 http://dx.doi.org/10.1251/bpo92 |
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author | Pick, Neora Cameron, Scott Arad, Dorit Av-Gay, Yossef |
author_facet | Pick, Neora Cameron, Scott Arad, Dorit Av-Gay, Yossef |
author_sort | Pick, Neora |
collection | PubMed |
description | The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to enter the market. As the development of new antibiotics is lengthy and costly, early monitoring of compound's toxicity is essential in the development of novel agents. Our interest is in a rapid, simple, high throughput screening method to assess cytotoxicity induced by potential agents. Some intracellular pathogens, such as Mycobacterium tuberculosis primary site of infection is human alveolar macrophages. Thus, evaluation of candidate drugs for macrophage toxicity is crucial. Protocols for high throughput drug toxicity screening of macrophages using flow cytometry are lacking in the literature. For this application we modified a preexisting technique, propidium iodide (PI) exclusion staining and utilized it for rapid toxicity tests. Samples were prepared in 96 well plates and analyzed by flow cytometry, which allowed for rapid, inexpensive and precise assessment of compound's toxicity associated with cell death. |
format | Text |
id | pubmed-521344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | Biological Procedures Online |
record_format | MEDLINE/PubMed |
spelling | pubmed-5213442004-10-06 Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry Pick, Neora Cameron, Scott Arad, Dorit Av-Gay, Yossef Biol Proced Online Research Article The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to enter the market. As the development of new antibiotics is lengthy and costly, early monitoring of compound's toxicity is essential in the development of novel agents. Our interest is in a rapid, simple, high throughput screening method to assess cytotoxicity induced by potential agents. Some intracellular pathogens, such as Mycobacterium tuberculosis primary site of infection is human alveolar macrophages. Thus, evaluation of candidate drugs for macrophage toxicity is crucial. Protocols for high throughput drug toxicity screening of macrophages using flow cytometry are lacking in the literature. For this application we modified a preexisting technique, propidium iodide (PI) exclusion staining and utilized it for rapid toxicity tests. Samples were prepared in 96 well plates and analyzed by flow cytometry, which allowed for rapid, inexpensive and precise assessment of compound's toxicity associated with cell death. Biological Procedures Online 2004-10-01 /pmc/articles/PMC521344/ /pubmed/15472722 http://dx.doi.org/10.1251/bpo92 Text en Copyright © October 10, 2004, N Pick et al. This paper is Open Access and is published in Biological Procedures Online under license from the authors. Copying, printing, redistribution and storage permitted. |
spellingShingle | Research Article Pick, Neora Cameron, Scott Arad, Dorit Av-Gay, Yossef Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry |
title | Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry |
title_full | Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry |
title_fullStr | Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry |
title_full_unstemmed | Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry |
title_short | Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry |
title_sort | screening of compounds toxicity against human monocytic cell line-thp-1 by flow cytometry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521344/ https://www.ncbi.nlm.nih.gov/pubmed/15472722 http://dx.doi.org/10.1251/bpo92 |
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