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Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation

Conventional bone tissue engineering approaches require isolation and in vitro propagation of autologous cells, followed by seeding on a variety of scaffolds. Those protracted procedures impede the clinical applications. Here we report the transdifferentiation of human fat tissue fragments retrieved...

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Autores principales: Bondarava, Maryna, Cattaneo, Chiara, Ren, Bin, Thasler, Wolfgang E., Jansson, Volkmar, Müller, Peter E., Betz, Oliver B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5213995/
https://www.ncbi.nlm.nih.gov/pubmed/28054585
http://dx.doi.org/10.1038/srep39712
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author Bondarava, Maryna
Cattaneo, Chiara
Ren, Bin
Thasler, Wolfgang E.
Jansson, Volkmar
Müller, Peter E.
Betz, Oliver B.
author_facet Bondarava, Maryna
Cattaneo, Chiara
Ren, Bin
Thasler, Wolfgang E.
Jansson, Volkmar
Müller, Peter E.
Betz, Oliver B.
author_sort Bondarava, Maryna
collection PubMed
description Conventional bone tissue engineering approaches require isolation and in vitro propagation of autologous cells, followed by seeding on a variety of scaffolds. Those protracted procedures impede the clinical applications. Here we report the transdifferentiation of human fat tissue fragments retrieved from subcutaneous fat into tissue with bone characteristics in vitro without prior cell isolation and propagation. 3D collagen-I cultures of human fat tissue were cultivated either in growth medium or in osteogenic medium (OM) with or without addition of Bone Morphogenetic Proteins (BMPs) BMP-2, BMP-7 or BMP-9. Ca(2+) depositions were observed after two weeks of osteogenic induction which visibly increased when either type of BMP was added. mRNA levels of alkaline phosphatase (ALP) and osteocalcin (OCN) increased when cultured in OM alone but addition of BMP-2, BMP-7 or BMP-9 caused significantly higher expression levels of ALP and OCN. Immunofluorescent staining for OCN, osteopontin and sclerostin supported the observed real-time-PCR data. BMP-9 was the most effective osteogenic inducer in this system. Our findings reveal that tissue regeneration can be remarkably simplified by omitting prior cell isolation and propagation, therefore removing significant obstacles on the way to clinical applications of much needed regeneration treatments.
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spelling pubmed-52139952017-01-09 Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation Bondarava, Maryna Cattaneo, Chiara Ren, Bin Thasler, Wolfgang E. Jansson, Volkmar Müller, Peter E. Betz, Oliver B. Sci Rep Article Conventional bone tissue engineering approaches require isolation and in vitro propagation of autologous cells, followed by seeding on a variety of scaffolds. Those protracted procedures impede the clinical applications. Here we report the transdifferentiation of human fat tissue fragments retrieved from subcutaneous fat into tissue with bone characteristics in vitro without prior cell isolation and propagation. 3D collagen-I cultures of human fat tissue were cultivated either in growth medium or in osteogenic medium (OM) with or without addition of Bone Morphogenetic Proteins (BMPs) BMP-2, BMP-7 or BMP-9. Ca(2+) depositions were observed after two weeks of osteogenic induction which visibly increased when either type of BMP was added. mRNA levels of alkaline phosphatase (ALP) and osteocalcin (OCN) increased when cultured in OM alone but addition of BMP-2, BMP-7 or BMP-9 caused significantly higher expression levels of ALP and OCN. Immunofluorescent staining for OCN, osteopontin and sclerostin supported the observed real-time-PCR data. BMP-9 was the most effective osteogenic inducer in this system. Our findings reveal that tissue regeneration can be remarkably simplified by omitting prior cell isolation and propagation, therefore removing significant obstacles on the way to clinical applications of much needed regeneration treatments. Nature Publishing Group 2017-01-05 /pmc/articles/PMC5213995/ /pubmed/28054585 http://dx.doi.org/10.1038/srep39712 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Bondarava, Maryna
Cattaneo, Chiara
Ren, Bin
Thasler, Wolfgang E.
Jansson, Volkmar
Müller, Peter E.
Betz, Oliver B.
Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation
title Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation
title_full Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation
title_fullStr Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation
title_full_unstemmed Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation
title_short Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation
title_sort osseous differentiation of human fat tissue grafts: from tissue engineering to tissue differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5213995/
https://www.ncbi.nlm.nih.gov/pubmed/28054585
http://dx.doi.org/10.1038/srep39712
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