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Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster

Optomotor behavior represents a stereotyped locomotor response to visual motion that is found in both vertebrate and invertebrate models. The Fly Stampede assay was developed to study an optomotor response in freely walking populations of Drosophila. Here we share optimized assay designs and softwar...

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Autores principales: Kim, Soomin, Tellez, Kelly, Buchan, Graham, Lebestky, Tim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214522/
https://www.ncbi.nlm.nih.gov/pubmed/28105003
http://dx.doi.org/10.3389/fnmol.2016.00148
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author Kim, Soomin
Tellez, Kelly
Buchan, Graham
Lebestky, Tim
author_facet Kim, Soomin
Tellez, Kelly
Buchan, Graham
Lebestky, Tim
author_sort Kim, Soomin
collection PubMed
description Optomotor behavior represents a stereotyped locomotor response to visual motion that is found in both vertebrate and invertebrate models. The Fly Stampede assay was developed to study an optomotor response in freely walking populations of Drosophila. Here we share optimized assay designs and software for production of a modified stampede assay that can be used for genetic screens, and improved tracking outputs for understanding behavioral parameters of visual-motion responses and arousal state of individual animals. Arousal state influences behavioral performance in the stampede assay. As proof of principle experiments we show parametric modulation of visual stimuli and startle stimuli in both wildtype and mutant flies for the type I family dopamine receptor Dop1R1 (DopR). DopR mutants are hyperactive and perform poorly in the stampede assay, suggesting a potential role in visual perception and/or arousal. The stampede assay creates an efficient platform for rapid screening of mutant animals or circuit manipulations for investigating attentional processes in Drosophila.
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spelling pubmed-52145222017-01-19 Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster Kim, Soomin Tellez, Kelly Buchan, Graham Lebestky, Tim Front Mol Neurosci Neuroscience Optomotor behavior represents a stereotyped locomotor response to visual motion that is found in both vertebrate and invertebrate models. The Fly Stampede assay was developed to study an optomotor response in freely walking populations of Drosophila. Here we share optimized assay designs and software for production of a modified stampede assay that can be used for genetic screens, and improved tracking outputs for understanding behavioral parameters of visual-motion responses and arousal state of individual animals. Arousal state influences behavioral performance in the stampede assay. As proof of principle experiments we show parametric modulation of visual stimuli and startle stimuli in both wildtype and mutant flies for the type I family dopamine receptor Dop1R1 (DopR). DopR mutants are hyperactive and perform poorly in the stampede assay, suggesting a potential role in visual perception and/or arousal. The stampede assay creates an efficient platform for rapid screening of mutant animals or circuit manipulations for investigating attentional processes in Drosophila. Frontiers Media S.A. 2016-12-27 /pmc/articles/PMC5214522/ /pubmed/28105003 http://dx.doi.org/10.3389/fnmol.2016.00148 Text en Copyright © 2016 Kim, Tellez, Buchan and Lebestky. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Kim, Soomin
Tellez, Kelly
Buchan, Graham
Lebestky, Tim
Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster
title Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster
title_full Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster
title_fullStr Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster
title_full_unstemmed Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster
title_short Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster
title_sort fly stampede 2.0: a next generation optomotor assay for walking behavior in drosophila melanogaster
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214522/
https://www.ncbi.nlm.nih.gov/pubmed/28105003
http://dx.doi.org/10.3389/fnmol.2016.00148
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