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Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster
Optomotor behavior represents a stereotyped locomotor response to visual motion that is found in both vertebrate and invertebrate models. The Fly Stampede assay was developed to study an optomotor response in freely walking populations of Drosophila. Here we share optimized assay designs and softwar...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214522/ https://www.ncbi.nlm.nih.gov/pubmed/28105003 http://dx.doi.org/10.3389/fnmol.2016.00148 |
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author | Kim, Soomin Tellez, Kelly Buchan, Graham Lebestky, Tim |
author_facet | Kim, Soomin Tellez, Kelly Buchan, Graham Lebestky, Tim |
author_sort | Kim, Soomin |
collection | PubMed |
description | Optomotor behavior represents a stereotyped locomotor response to visual motion that is found in both vertebrate and invertebrate models. The Fly Stampede assay was developed to study an optomotor response in freely walking populations of Drosophila. Here we share optimized assay designs and software for production of a modified stampede assay that can be used for genetic screens, and improved tracking outputs for understanding behavioral parameters of visual-motion responses and arousal state of individual animals. Arousal state influences behavioral performance in the stampede assay. As proof of principle experiments we show parametric modulation of visual stimuli and startle stimuli in both wildtype and mutant flies for the type I family dopamine receptor Dop1R1 (DopR). DopR mutants are hyperactive and perform poorly in the stampede assay, suggesting a potential role in visual perception and/or arousal. The stampede assay creates an efficient platform for rapid screening of mutant animals or circuit manipulations for investigating attentional processes in Drosophila. |
format | Online Article Text |
id | pubmed-5214522 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52145222017-01-19 Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster Kim, Soomin Tellez, Kelly Buchan, Graham Lebestky, Tim Front Mol Neurosci Neuroscience Optomotor behavior represents a stereotyped locomotor response to visual motion that is found in both vertebrate and invertebrate models. The Fly Stampede assay was developed to study an optomotor response in freely walking populations of Drosophila. Here we share optimized assay designs and software for production of a modified stampede assay that can be used for genetic screens, and improved tracking outputs for understanding behavioral parameters of visual-motion responses and arousal state of individual animals. Arousal state influences behavioral performance in the stampede assay. As proof of principle experiments we show parametric modulation of visual stimuli and startle stimuli in both wildtype and mutant flies for the type I family dopamine receptor Dop1R1 (DopR). DopR mutants are hyperactive and perform poorly in the stampede assay, suggesting a potential role in visual perception and/or arousal. The stampede assay creates an efficient platform for rapid screening of mutant animals or circuit manipulations for investigating attentional processes in Drosophila. Frontiers Media S.A. 2016-12-27 /pmc/articles/PMC5214522/ /pubmed/28105003 http://dx.doi.org/10.3389/fnmol.2016.00148 Text en Copyright © 2016 Kim, Tellez, Buchan and Lebestky. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Kim, Soomin Tellez, Kelly Buchan, Graham Lebestky, Tim Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster |
title | Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster |
title_full | Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster |
title_fullStr | Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster |
title_full_unstemmed | Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster |
title_short | Fly Stampede 2.0: A Next Generation Optomotor Assay for Walking Behavior in Drosophila Melanogaster |
title_sort | fly stampede 2.0: a next generation optomotor assay for walking behavior in drosophila melanogaster |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214522/ https://www.ncbi.nlm.nih.gov/pubmed/28105003 http://dx.doi.org/10.3389/fnmol.2016.00148 |
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