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Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae)
The tea of aerial parts of Smilax larvata Griseb. (Smilacaceae) has been ethnopharmacologically used in Southern Brazil due to its anti-inflammatory action. In this study, ethanolic and organic extracts from aerial parts of S. larvata were phytochemically and pharmacologically characterized. The phy...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214527/ https://www.ncbi.nlm.nih.gov/pubmed/28101120 http://dx.doi.org/10.1155/2016/9894610 |
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author | Hirota, Beatriz Cristina Konopatzki Paula, Cristiane da Silva de Oliveira, Vinícius Bednarczuk da Cunha, Joice Maria Schreiber, Anne Karoline Ocampos, Fernanda Maria Marins Barison, Anderson Miguel, Obdulio Gomes Miguel, Marilis Dallarmi |
author_facet | Hirota, Beatriz Cristina Konopatzki Paula, Cristiane da Silva de Oliveira, Vinícius Bednarczuk da Cunha, Joice Maria Schreiber, Anne Karoline Ocampos, Fernanda Maria Marins Barison, Anderson Miguel, Obdulio Gomes Miguel, Marilis Dallarmi |
author_sort | Hirota, Beatriz Cristina Konopatzki |
collection | PubMed |
description | The tea of aerial parts of Smilax larvata Griseb. (Smilacaceae) has been ethnopharmacologically used in Southern Brazil due to its anti-inflammatory action. In this study, ethanolic and organic extracts from aerial parts of S. larvata were phytochemically and pharmacologically characterized. The phytochemical analysis of EtOAc extract of S. larvata revealed the presence of three flavonoids, drabanemoroside, kaempferol 3-O-α-L-rhamnopyranosyl(1→2)-α-L-rhamnopyranoside, and kaempferol, the first two being isolated for the first time in this genus, two phenolic compounds p-hydroxybenzoic acid and p-coumaric acid, and alkaloids. In vitro assays demonstrated a potential antioxidant property of SLG. The treatment with SLG induced a significant reduction of the formalin-evoked flinches in rats, an effect reversed by opioid antagonist naloxone. Treatment with SLG also induced a significant increase in the hot plate latency and a decrease of intestinal motility by 45%. No effect was observed over nociceptive responses induced by a TRPA1 agonist mustard oil or over acetic acid-induced writhing in mice. Together, our data suggested that SLG has an in vivo antinociceptive effect, which seems to be associated with the opioid system activation. These findings support previous claims of medical use of Smilax larvata in the treatment of pain conditions. |
format | Online Article Text |
id | pubmed-5214527 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-52145272017-01-18 Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae) Hirota, Beatriz Cristina Konopatzki Paula, Cristiane da Silva de Oliveira, Vinícius Bednarczuk da Cunha, Joice Maria Schreiber, Anne Karoline Ocampos, Fernanda Maria Marins Barison, Anderson Miguel, Obdulio Gomes Miguel, Marilis Dallarmi Evid Based Complement Alternat Med Research Article The tea of aerial parts of Smilax larvata Griseb. (Smilacaceae) has been ethnopharmacologically used in Southern Brazil due to its anti-inflammatory action. In this study, ethanolic and organic extracts from aerial parts of S. larvata were phytochemically and pharmacologically characterized. The phytochemical analysis of EtOAc extract of S. larvata revealed the presence of three flavonoids, drabanemoroside, kaempferol 3-O-α-L-rhamnopyranosyl(1→2)-α-L-rhamnopyranoside, and kaempferol, the first two being isolated for the first time in this genus, two phenolic compounds p-hydroxybenzoic acid and p-coumaric acid, and alkaloids. In vitro assays demonstrated a potential antioxidant property of SLG. The treatment with SLG induced a significant reduction of the formalin-evoked flinches in rats, an effect reversed by opioid antagonist naloxone. Treatment with SLG also induced a significant increase in the hot plate latency and a decrease of intestinal motility by 45%. No effect was observed over nociceptive responses induced by a TRPA1 agonist mustard oil or over acetic acid-induced writhing in mice. Together, our data suggested that SLG has an in vivo antinociceptive effect, which seems to be associated with the opioid system activation. These findings support previous claims of medical use of Smilax larvata in the treatment of pain conditions. Hindawi Publishing Corporation 2016 2016-12-22 /pmc/articles/PMC5214527/ /pubmed/28101120 http://dx.doi.org/10.1155/2016/9894610 Text en Copyright © 2016 Beatriz Cristina Konopatzki Hirota et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hirota, Beatriz Cristina Konopatzki Paula, Cristiane da Silva de Oliveira, Vinícius Bednarczuk da Cunha, Joice Maria Schreiber, Anne Karoline Ocampos, Fernanda Maria Marins Barison, Anderson Miguel, Obdulio Gomes Miguel, Marilis Dallarmi Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae) |
title | Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae) |
title_full | Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae) |
title_fullStr | Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae) |
title_full_unstemmed | Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae) |
title_short | Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae) |
title_sort | phytochemical and antinociceptive, anti-inflammatory, and antioxidant studies of smilax larvata (smilacaceae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214527/ https://www.ncbi.nlm.nih.gov/pubmed/28101120 http://dx.doi.org/10.1155/2016/9894610 |
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