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Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling

Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a neces...

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Detalles Bibliográficos
Autores principales: Shao, Weiping, Zhou, Zhimin, Laroche, Isabelle, Lu, Hong, Zong, Qiuling, Patel, Dhavalkumar D., Kingsmore, Stephen, Piccoli, Steven P.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521503/
https://www.ncbi.nlm.nih.gov/pubmed/14688416
http://dx.doi.org/10.1155/S1110724303209268
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author Shao, Weiping
Zhou, Zhimin
Laroche, Isabelle
Lu, Hong
Zong, Qiuling
Patel, Dhavalkumar D.
Kingsmore, Stephen
Piccoli, Steven P.
author_facet Shao, Weiping
Zhou, Zhimin
Laroche, Isabelle
Lu, Hong
Zong, Qiuling
Patel, Dhavalkumar D.
Kingsmore, Stephen
Piccoli, Steven P.
author_sort Shao, Weiping
collection PubMed
description Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a necessity for the effective advancement of understanding disease processes. In this paper, we describe the generation of highly multiplexed, antibody-based, specific, and sensitive protein microarrays coupled with rolling-circle signal amplification (RCA) technology. A total of 150 cytokines were simultaneously detected in an RCA sandwich immunoassay format. Greater than half of these proteins have detection sensitivities in the pg/mL range. The validation of antibody microarray with human serum indicated that RCA-based protein microarrays are a powerful tool for high-throughput analysis of protein expression and molecular diagnostics.
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spelling pubmed-5215032004-12-06 Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling Shao, Weiping Zhou, Zhimin Laroche, Isabelle Lu, Hong Zong, Qiuling Patel, Dhavalkumar D. Kingsmore, Stephen Piccoli, Steven P. J Biomed Biotechnol Research Article Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a necessity for the effective advancement of understanding disease processes. In this paper, we describe the generation of highly multiplexed, antibody-based, specific, and sensitive protein microarrays coupled with rolling-circle signal amplification (RCA) technology. A total of 150 cytokines were simultaneously detected in an RCA sandwich immunoassay format. Greater than half of these proteins have detection sensitivities in the pg/mL range. The validation of antibody microarray with human serum indicated that RCA-based protein microarrays are a powerful tool for high-throughput analysis of protein expression and molecular diagnostics. Hindawi Publishing Corporation 2003-12-04 /pmc/articles/PMC521503/ /pubmed/14688416 http://dx.doi.org/10.1155/S1110724303209268 Text en Copyright © 2003, Hindawi Publishing Corporation
spellingShingle Research Article
Shao, Weiping
Zhou, Zhimin
Laroche, Isabelle
Lu, Hong
Zong, Qiuling
Patel, Dhavalkumar D.
Kingsmore, Stephen
Piccoli, Steven P.
Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling
title Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling
title_full Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling
title_fullStr Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling
title_full_unstemmed Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling
title_short Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling
title_sort optimization of rolling-circle amplified protein microarrays for multiplexed protein profiling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521503/
https://www.ncbi.nlm.nih.gov/pubmed/14688416
http://dx.doi.org/10.1155/S1110724303209268
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