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Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling
Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a neces...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521503/ https://www.ncbi.nlm.nih.gov/pubmed/14688416 http://dx.doi.org/10.1155/S1110724303209268 |
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author | Shao, Weiping Zhou, Zhimin Laroche, Isabelle Lu, Hong Zong, Qiuling Patel, Dhavalkumar D. Kingsmore, Stephen Piccoli, Steven P. |
author_facet | Shao, Weiping Zhou, Zhimin Laroche, Isabelle Lu, Hong Zong, Qiuling Patel, Dhavalkumar D. Kingsmore, Stephen Piccoli, Steven P. |
author_sort | Shao, Weiping |
collection | PubMed |
description | Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a necessity for the effective advancement of understanding disease processes. In this paper, we describe the generation of highly multiplexed, antibody-based, specific, and sensitive protein microarrays coupled with rolling-circle signal amplification (RCA) technology. A total of 150 cytokines were simultaneously detected in an RCA sandwich immunoassay format. Greater than half of these proteins have detection sensitivities in the pg/mL range. The validation of antibody microarray with human serum indicated that RCA-based protein microarrays are a powerful tool for high-throughput analysis of protein expression and molecular diagnostics. |
format | Text |
id | pubmed-521503 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-5215032004-12-06 Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling Shao, Weiping Zhou, Zhimin Laroche, Isabelle Lu, Hong Zong, Qiuling Patel, Dhavalkumar D. Kingsmore, Stephen Piccoli, Steven P. J Biomed Biotechnol Research Article Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a necessity for the effective advancement of understanding disease processes. In this paper, we describe the generation of highly multiplexed, antibody-based, specific, and sensitive protein microarrays coupled with rolling-circle signal amplification (RCA) technology. A total of 150 cytokines were simultaneously detected in an RCA sandwich immunoassay format. Greater than half of these proteins have detection sensitivities in the pg/mL range. The validation of antibody microarray with human serum indicated that RCA-based protein microarrays are a powerful tool for high-throughput analysis of protein expression and molecular diagnostics. Hindawi Publishing Corporation 2003-12-04 /pmc/articles/PMC521503/ /pubmed/14688416 http://dx.doi.org/10.1155/S1110724303209268 Text en Copyright © 2003, Hindawi Publishing Corporation |
spellingShingle | Research Article Shao, Weiping Zhou, Zhimin Laroche, Isabelle Lu, Hong Zong, Qiuling Patel, Dhavalkumar D. Kingsmore, Stephen Piccoli, Steven P. Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling |
title | Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling |
title_full | Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling |
title_fullStr | Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling |
title_full_unstemmed | Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling |
title_short | Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling |
title_sort | optimization of rolling-circle amplified protein microarrays for multiplexed protein profiling |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521503/ https://www.ncbi.nlm.nih.gov/pubmed/14688416 http://dx.doi.org/10.1155/S1110724303209268 |
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